CT10: a new cancer-testis (CT) antigen homologous to CT7 and the MAGE family, identified by representational-difference analysis

Int J Cancer. 2000 Mar 1;85(5):726-32. doi: 10.1002/(sici)1097-0215(20000301)85:5<726::aid-ijc21>3.0.co;2-f.

Abstract

Assays relying on humoral or T-cell-based recognition of tumor antigens to identify potential targets for immunotherapy have led to the discovery of a significant number of immunogenic gene products, including cancer-testis (CT) antigens predominantly expressed in cancer cells and male germ cells. The search for cancer-specific antigens has been extended via the technique of representational-difference analysis and SK-MEL-37, a melanoma cell line expressing a broad range of CT antigens. Using this approach, we have isolated CT antigen genes, genes over-expressed in cancer, e. g., PRAME and KOC, and genes encoding neuro-ectodermal markers. The identified CT antigen genes include the previously defined MAGE-A6, MAGE-A4a, MAGE-A10, CT7/MAGE-C1, as well as a novel gene designated CT10, which shows strong homology to CT7/MAGE-C1 both at cDNA and at genomic levels. Chromosome mapping localized CT10 to Xq27, in close proximity to CT7/MAGE-C1 and MAGE-A genes. CT10 mRNA is expressed in testis and in 20 to 30% of various human cancers. A serological survey identified 2 melanoma patients with anti-CT10 antibody, demonstrating the immunogenicity of CT10 in humans.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Neoplasm / analysis
  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Exons
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Introns
  • Male
  • Melanoma / genetics*
  • Molecular Sequence Data
  • Neoplasm Proteins / analysis
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / genetics*
  • Organ Specificity
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Testis / metabolism
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • Antigens, Neoplasm
  • MAGEC2 protein, human
  • Neoplasm Proteins
  • RNA, Messenger