Abstract
We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H(+)-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging / genetics*
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Amino Acid Sequence
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Animals
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Base Sequence
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Biomarkers*
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Blotting, Northern
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Cell Survival / drug effects
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary / analysis
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Gene Expression Regulation*
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In Vitro Techniques
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Male
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Molecular Sequence Data
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Neurons / cytology
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Neurons / drug effects
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Oligonucleotides, Antisense / pharmacology
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Prefrontal Cortex / metabolism*
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Protein Biosynthesis
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Proteins / genetics*
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Proton-Translocating ATPases / chemistry
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Proton-Translocating ATPases / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Sequence Homology, Amino Acid
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Tissue Distribution
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Vacuolar Proton-Translocating ATPases*
Substances
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Atp6ap1 protein, rat
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Biomarkers
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DNA, Complementary
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Oligonucleotides, Antisense
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Proteins
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RNA, Messenger
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Vacuolar Proton-Translocating ATPases
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Proton-Translocating ATPases