Cutting edge: identification of GL50, a novel B7-like protein that functionally binds to ICOS receptor

V Ling; P W Wu; H F Finnerty; K M Bean; V Spaulding; L A Fouser; J P Leonard; S E Hunter; R Zollner; J L Thomas; J S Miyashiro; K A Jacobs; M Collins

doi:10.4049/jimmunol.164.4.1653

Cutting edge: identification of GL50, a novel B7-like protein that functionally binds to ICOS receptor

J Immunol. 2000 Feb 15;164(4):1653-7. doi: 10.4049/jimmunol.164.4.1653.

Authors

V Ling¹, P W Wu, H F Finnerty, K M Bean, V Spaulding, L A Fouser, J P Leonard, S E Hunter, R Zollner, J L Thomas, J S Miyashiro, K A Jacobs, M Collins

Affiliation

¹ Department of Immunology, Genetics Institute, Cambridge, MA 02081, USA. VLing@genetics.com

PMID: 10657606
DOI: 10.4049/jimmunol.164.4.1653

Abstract

By the genetic selection of mouse cDNAs encoding secreted proteins, a B7-like cDNA clone termed mouse GL50 (mGL50) was isolated encoding a 322-aa polypeptide identical with B7h. Isolation of the human ortholog of this cDNA (hGL50) revealed a coding sequence of 309 aa residues with 42% sequence identity with mGL50. Northern analysis indicated GL50 to be present in many tissues including lymphoid, embryonic yolk sac, and fetal liver samples. Of the CD28, CTLA4, and ICOS fusion constructs tested, flow cytometric analysis demonstrated only mouse ICOS-IgG binding to mGL50 cell transfectants. Subsequent phenotyping demonstrated high levels of ICOS ligand staining on splenic CD19+ B cells and low levels on CD3+ T cells. These results indicate that GL50 is a specific ligand for the ICOS receptor and suggest that the GL50-ICOS interaction functions in lymphocyte costimulation.

MeSH terms

Amino Acid Sequence
Animals
Antigens, CD / chemistry
Antigens, CD / genetics
Antigens, CD / isolation & purification*
Antigens, CD / metabolism
Antigens, Differentiation, T-Lymphocyte / metabolism*
B7-1 Antigen / chemistry
B7-1 Antigen / genetics
B7-1 Antigen / isolation & purification*
B7-1 Antigen / metabolism
B7-2 Antigen
Blotting, Northern
Cloning, Molecular
DNA, Complementary / isolation & purification
Humans
Inducible T-Cell Co-Stimulator Ligand
Inducible T-Cell Co-Stimulator Protein
Ligands
Lymph Nodes / chemistry
Lymph Nodes / metabolism
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / genetics
Membrane Glycoproteins / isolation & purification*
Membrane Glycoproteins / metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Proteins / chemistry
Sequence Alignment
Transcription, Genetic / immunology
Tumor Cells, Cultured

Substances

Antigens, CD
Antigens, Differentiation, T-Lymphocyte
B7-1 Antigen
B7-2 Antigen
CD86 protein, human
Cd86 protein, mouse
DNA, Complementary
ICOS protein, human
ICOSLG protein, human
Icos protein, mouse
Icosl protein, mouse
Inducible T-Cell Co-Stimulator Ligand
Inducible T-Cell Co-Stimulator Protein
Ligands
Membrane Glycoproteins
Proteins