Cloning and characterization of the human novel gene encoding 6.2 kDa protein highly expressed upon ultraviolet irradiation in DNA-PK-deleted human glioma M059J cells

DNA Seq. 1999;10(3):195-8. doi: 10.3109/10425179909033947.

Abstract

The full length of a gene isolated using a methylated DNA binding column as previously reported (Cross et al., 1994) has been cloned. The gene encodes 55 amino acids (6.2 kDa), and has a transcript of approximately 0.45 kb. It has 2 exons in the genome. After searching for sequence homology (BLAST), a homology to hypothetically 6.3 kDa protein located at cosmid ZK652 (protein P34660; EMBL Accession No. L14429) of C. elegans chromosome 3 was noted (51.9% amino acid homology). It has 1 site (-SQ-) for DNA-dependent protein kinase (DNA-PK) phosphorylation and 2 sites of myristilation. This novel gene is very strongly expressed (about 25.6 times) in DNA-PK-deleted human glioma cell line (M059J) 2 hrs after ultraviolet (UV) irradiation compared to the DNA-PK +/+ cell line (M059K) which expresses the gene at a relatively low level (about 7.5 times). Therefore, this result suggests that DNA-PK may negatively regulate the transcriptional level of this gene in M059K cells upon UV damage (50 J/m2), associated with cell toxicity.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Survival / radiation effects
  • Central Nervous System Neoplasms / genetics*
  • Central Nervous System Neoplasms / radiotherapy*
  • Cloning, Molecular
  • DNA Damage / radiation effects
  • DNA Methylation
  • DNA-Activated Protein Kinase
  • DNA-Binding Proteins*
  • Dose-Response Relationship, Radiation
  • Gene Expression Regulation, Neoplastic / radiation effects
  • Glioma / enzymology
  • Glioma / genetics*
  • Glioma / radiotherapy*
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism
  • Humans
  • Membrane Proteins
  • Mitochondrial Precursor Protein Import Complex Proteins
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Nuclear Proteins
  • Phosphorylation
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism
  • Protein Serine-Threonine Kinases / radiation effects
  • Proteins / genetics*
  • Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured
  • Ultraviolet Rays

Substances

  • DNA-Binding Proteins
  • Helminth Proteins
  • Membrane Proteins
  • Mitochondrial Precursor Protein Import Complex Proteins
  • Mitochondrial Proteins
  • Nuclear Proteins
  • Proteins
  • TOMM7 protein, human
  • DNA-Activated Protein Kinase
  • PRKDC protein, human
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/AJ011007