Proteinases in developing dental enamel

Crit Rev Oral Biol Med. 1999;10(4):425-41. doi: 10.1177/10454411990100040101.

Abstract

For almost three decades, proteinases have been known to reside within developing dental enamel. However, identification and characterization of these proteinases have been slow and difficult, because they are present in very small quantities and they are difficult to purify directly from the mineralizing enamel. Enamel matrix proteins such as amelogenin, ameloblastin, and enamelin are cleaved by proteinases soon after they are secreted, and their cleavage products accumulate in the deeper, more mature enamel layers, while the full-length proteins are observed only at the surface. These results suggest that proteinases are necessary for "activating" enamel proteins so the parent proteins and their cleavage products may perform different functions. A novel matrix metalloproteinase named enamelysin (MMP-20) was recently cloned from tooth tissues and was later shown to localize primarily within the most recently formed enamel. Furthermore, recombinant porcine enamelysin was demonstrated to cleave recombinant porcine amelogenin at virtually all of the sites that have previously been described in vivo. Therefore, enamelysin is at least one enzyme that may be important during early enamel development. As enamel development progresses to the later stages, a profound decrease in the enamel protein content is observed. Proteinases have traditionally been assumed to degrade the organic matrix prior to its removal from the enamel. Recently, a novel serine proteinase named enamel matrix serine proteinase-1 (EMSP1) was cloned from enamel organ epithelia. EMSP1 localizes primarily to the early maturation stage enamel and may, therefore, be involved in the degradation of proteins prior to their removal from the maturing enamel. Other, as yet unidentified, proteinases and proteinase inhibitors are almost certainly present within the forming enamel and await discovery.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Amelogenesis / physiology*
  • Amelogenin
  • Animals
  • Dental Enamel / enzymology*
  • Dental Enamel Proteins / isolation & purification
  • Dental Enamel Proteins / metabolism
  • Enamel Organ / enzymology
  • Endopeptidases / analysis
  • Endopeptidases / isolation & purification*
  • Humans
  • Kallikreins*
  • Matrix Metalloproteinase 20
  • Matrix Metalloproteinases / isolation & purification
  • Matrix Metalloproteinases / metabolism
  • Serine Endopeptidases / isolation & purification
  • Serine Endopeptidases / metabolism
  • Swine
  • Tooth Germ / metabolism

Substances

  • AMBN protein, human
  • Amelogenin
  • Dental Enamel Proteins
  • ameloblastin protein, Sus scrofa
  • tuftelin
  • Endopeptidases
  • Kallikreins
  • Serine Endopeptidases
  • kallikrein 4
  • MMP20 protein, human
  • Matrix Metalloproteinase 20
  • Matrix Metalloproteinases