Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3

N Kelkar; S Gupta; M Dickens; R J Davis

doi:10.1128/MCB.20.3.1030-1043.2000

Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3

Mol Cell Biol. 2000 Feb;20(3):1030-43. doi: 10.1128/MCB.20.3.1030-1043.2000.

Authors

N Kelkar¹, S Gupta, M Dickens, R J Davis

Affiliation

¹ Howard Hughes Medical Institute, Program in Molecular Medicine, Department of Biochemistry, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Abstract

The c-Jun NH(2)-terminal kinase (JNK) group of mitogen-activated protein kinases (MAPKs) is activated in response to the treatment of cells with inflammatory cytokines and by exposure to environmental stress. JNK activation is mediated by a protein kinase cascade composed of a MAPK kinase and a MAPK kinase kinase. Here we describe the molecular cloning of a putative molecular scaffold protein, JIP3, that binds the protein kinase components of a JNK signaling module and facilitates JNK activation in cultured cells. JIP3 is expressed in the brain and at lower levels in the heart and other tissues. Immunofluorescence analysis demonstrated that JIP3 was present in the cytoplasm and accumulated in the growth cones of developing neurites. JIP3 is a member of a novel class of putative MAPK scaffold proteins that may regulate signal transduction by the JNK pathway.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adaptor Proteins, Signal Transducing*
Aging
Amino Acid Sequence
Animals
Brain / metabolism*
Cells, Cultured
Cloning, Molecular
Embryonic and Fetal Development
Enzyme Activation
Female
Gene Expression Regulation, Developmental*
Gene Library
JNK Mitogen-Activated Protein Kinases
MAP Kinase Kinase Kinases / metabolism
Male
Mice
Mitogen-Activated Protein Kinases / chemistry
Mitogen-Activated Protein Kinases / metabolism*
Molecular Sequence Data
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Neurons / metabolism
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Signal Transduction

Substances

Adaptor Proteins, Signal Transducing
Mapk8ip3 protein, mouse
Nerve Tissue Proteins
Protein Isoforms
Recombinant Proteins
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
MAP Kinase Kinase Kinases