Pegylation of magnetically oriented lipid bilayers

J Magn Reson. 2000 Jan;142(1):177-82. doi: 10.1006/jmre.1999.1928.

Abstract

We report NMR data for magnetically oriented phospholipid bilayers which have been doped with a lipid derivatized with a polyethylene glycol polymer headgroup to stabilize samples against aggregation. (13)C, (31)P, and (2)H NMR data indicate that the incorporation of PEG2000-PE (1% molar to DMPC) does not interfere with the orientation properties of bicelles prepared at 25% w/v with or without the presence of lanthanide. Bicelles prepared at 10% w/v are also shown to orient when PEG2000-PE is added. The addition of PEG2000-PE to cholesterol-containing, lanthanide-flipped bicelles is shown to inhibit sample phase separation and improve spectral quality. Furthermore, the addition of PEG2000-PE to high w/v bicelles (40% w/v) is demonstrated to lead to an increase in overall sample order.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cholesterol
  • Lipid Bilayers / chemistry*
  • Magnetic Resonance Spectroscopy
  • Metals, Rare Earth
  • Phosphatidylethanolamines
  • Phospholipid Ethers
  • Polyethylene Glycols

Substances

  • 1,2-dihexadecyl-sn-glycero-3-phosphocholine
  • Lipid Bilayers
  • Metals, Rare Earth
  • Phosphatidylethanolamines
  • Phospholipid Ethers
  • dioleoyl-N-(monomethoxypolyethylene glycol succinyl)phosphatidylethanolamine
  • Polyethylene Glycols
  • Cholesterol