Abstract
Bcl-2 is the best characterized inhibitor of apoptosis, although the molecular basis of this action is not fully understood. Using a protein interaction cloning procedure, we identified a human gene designated as bis (mapped to chromosome 10q25) that encoded a novel Bcl-2-interacting protein. Bis protein showed no significant homology with Bcl-2 family proteins and had no prominent functional motif. Co-immunoprecipitation analysis confirmed that Bis interacted with Bcl-2 in vivo. DNA transfection experiments indicated that Bis itself exerted only weak anti-apoptotic activity, but was synergistic with Bcl-2 in preventing Bax-induced and Fas-mediated apoptosis. These results suggest that Bis is a novel modulator of cellular anti-apoptotic activity that functions through its interaction with Bcl-2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Animals
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Apoptosis / physiology*
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Apoptosis Regulatory Proteins
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B-Lymphocytes / chemistry
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Binding Sites
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Carrier Proteins / genetics
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Carrier Proteins / isolation & purification*
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Carrier Proteins / metabolism
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Chromosome Mapping
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Chromosomes, Human, Pair 10 / genetics*
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Cloning, Molecular
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DNA, Complementary / genetics
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Genes*
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Humans
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Mice
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Molecular Sequence Data
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Organ Specificity
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Protein Structure, Tertiary
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Proto-Oncogene Proteins c-bcl-2 / physiology*
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Sequence Alignment
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Sequence Homology, Amino Acid
Substances
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Adaptor Proteins, Signal Transducing
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Apoptosis Regulatory Proteins
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BAG3 protein, human
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Bag3 protein, mouse
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Carrier Proteins
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DNA, Complementary
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Proto-Oncogene Proteins c-bcl-2
Associated data
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GENBANK/AF127139
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GENBANK/AF130471