Cutting edge: KAP10, a novel transmembrane adapter protein genetically linked to DAP12 but with unique signaling properties

J Immunol. 1999 Nov 1;163(9):4651-4.

Abstract

Transmembrane adapter proteins are a class of molecules that mediate signals from an extracellular receptor to the cytoplasm of the cell. We have cloned a novel transmembrane adapter protein called KAP10, a approximately 10-kDa protein that is encoded within 100 bp of the DAP12 locus on human chromosome 19. KAP10 is predominantly expressed in immune cells, including NK cells, T cells, and monocytes. We show that KAP10, unlike other transmembrane adapter proteins, binds phosphatidylinositol-3 kinase following phosphorylation of a cytoplasmic YINM motif, which results in activation of Akt. In addition, we identify KAP10 as being able to bind the adapter protein Grb2. Based on our data, we suggest that this molecule is involved in stimulation and costimulation in cells of both myeloid and lymphoid origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CD4 Antigens / immunology
  • Cell Line
  • Cell Lineage / genetics
  • Cell Lineage / immunology
  • Enzyme Activation
  • Genetic Linkage / immunology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Immune Sera / metabolism
  • Jurkat Cells
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / metabolism
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics*
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Phosphatidylinositol 3-Kinases / metabolism
  • Protein Binding / immunology
  • Protein Serine-Threonine Kinases / metabolism
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins*
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Signal Transduction / genetics
  • Signal Transduction / immunology*
  • U937 Cells

Substances

  • Adaptor Proteins, Signal Transducing
  • CD4 Antigens
  • HCST protein, human
  • Hcst protein, mouse
  • Immune Sera
  • Membrane Proteins
  • Proto-Oncogene Proteins
  • Receptors, Immunologic
  • TYROBP protein, human
  • AKT1 protein, human
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt

Associated data

  • GENBANK/AF172929
  • GENBANK/AF172930