Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase

Biochim Biophys Acta. 1999 Sep 3;1446(3):383-8. doi: 10.1016/s0167-4781(99)00105-0.

Abstract

The isolation and expression of a human liver cDNA encoding a 40-kDa protein with glyoxylate and hydroxypyruvate reductase activities is described. The cDNA (GLXR) is 1235 bp and consists of a predicted open reading frame of 987 bp with a 225-bp 3'-untranslated region. The 328-amino acid protein has partial sequence similarity to hydroxypyruvate and glyoxylate reductases from a variety of plant and microbial species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Carbohydrate Dehydrogenases / genetics
  • Cell Line
  • Cytosol / metabolism
  • DNA, Complementary / chemistry*
  • Gene Expression
  • Humans
  • Hydroxypyruvate Reductase
  • Hyperoxaluria, Primary / enzymology
  • Hyperoxaluria, Primary / genetics
  • Liver / enzymology*
  • Molecular Sequence Data
  • Peroxisomes / metabolism
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins / genetics*
  • Sequence Alignment
  • Transaminases / metabolism
  • Transfection

Substances

  • DNA, Complementary
  • Recombinant Fusion Proteins
  • Alcohol Oxidoreductases
  • Carbohydrate Dehydrogenases
  • glyoxylate reductase
  • Glycerate dehydrogenase
  • Hydroxypyruvate Reductase
  • glycollate oxidase
  • Transaminases
  • Alanine-glyoxylate transaminase

Associated data

  • GENBANK/AF134895