Abstract
The isolation and expression of a human liver cDNA encoding a 40-kDa protein with glyoxylate and hydroxypyruvate reductase activities is described. The cDNA (GLXR) is 1235 bp and consists of a predicted open reading frame of 987 bp with a 225-bp 3'-untranslated region. The 328-amino acid protein has partial sequence similarity to hydroxypyruvate and glyoxylate reductases from a variety of plant and microbial species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / genetics*
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Alcohol Oxidoreductases / metabolism
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Amino Acid Sequence
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Base Sequence
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Carbohydrate Dehydrogenases / genetics
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Cell Line
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Cytosol / metabolism
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DNA, Complementary / chemistry*
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Gene Expression
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Humans
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Hydroxypyruvate Reductase
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Hyperoxaluria, Primary / enzymology
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Hyperoxaluria, Primary / genetics
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Liver / enzymology*
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Molecular Sequence Data
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Peroxisomes / metabolism
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Polymerase Chain Reaction
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Recombinant Fusion Proteins / genetics*
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Sequence Alignment
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Transaminases / metabolism
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Transfection
Substances
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DNA, Complementary
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Recombinant Fusion Proteins
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Alcohol Oxidoreductases
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Carbohydrate Dehydrogenases
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glyoxylate reductase
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Glycerate dehydrogenase
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Hydroxypyruvate Reductase
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glycollate oxidase
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Transaminases
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Alanine-glyoxylate transaminase