Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum

FEBS Lett. 1999 Sep 3;457(3):316-22. doi: 10.1016/s0014-5793(99)01075-3.

Abstract

Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and immunoblotting. Among the proteins characterised are the Sec61p complex, the oligosaccharyl transferase (OST) complex, the translocon-associated protein (TRAP) complex, the TRAM and RAMP4 proteins, the signal recognition particle (SRP) and the SRP receptor (SR). Interestingly, the RAMP4 protein, SR and OST complex display more than one oligomeric form.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Calcium-Binding Proteins / metabolism
  • Calreticulin
  • Dogs
  • Electrophoresis, Polyacrylamide Gel / methods
  • Endoplasmic Reticulum, Rough / metabolism*
  • Immunoblotting
  • Intracellular Membranes / metabolism*
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins / immunology
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism
  • Protein Disulfide-Isomerases / metabolism
  • Proteins / immunology
  • Proteins / metabolism*
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Receptors, Peptide / chemistry
  • Receptors, Peptide / metabolism*
  • Ribonucleoproteins / metabolism
  • Ribosomes / metabolism
  • SEC Translocation Channels

Substances

  • Calcium-Binding Proteins
  • Calreticulin
  • Membrane Glycoproteins
  • Membrane Proteins
  • Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Peptide
  • Ribonucleoproteins
  • SEC Translocation Channels
  • endoplasmic reticulum glycoprotein p72
  • ribophorin
  • signal peptide receptor
  • signal sequence receptor
  • Protein Disulfide-Isomerases