Development of a new DNA sequencing method: 3'-ester cleavage catalyzed by Taq DNA polymerase

Nucleosides Nucleotides. 1999 Apr-May;18(4-5):1021-2. doi: 10.1080/15257779908041636.

Abstract

A new 3'-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.

MeSH terms

  • Catalysis
  • Esters
  • Hydrolysis
  • Sequence Analysis, DNA / methods*
  • Taq Polymerase / metabolism*

Substances

  • Esters
  • Taq Polymerase