F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons

Y Feinstein; V Borrell; C Garcia; T Burstyn-Cohen; V Tzarfaty; A Frumkin; A Nose; H Okamoto; S Higashijima; E Soriano; A Klar

doi:10.1242/dev.126.16.3637

F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons

Development. 1999 Aug;126(16):3637-48. doi: 10.1242/dev.126.16.3637.

Authors

Y Feinstein¹, V Borrell, C Garcia, T Burstyn-Cohen, V Tzarfaty, A Frumkin, A Nose, H Okamoto, S Higashijima, E Soriano, A Klar

Affiliation

¹ Department of Anatomy and Cell Biology, The Hebrew University-Hadassah Medical School, Jerusalem, PO Box 12272, 91120 Israel.

PMID: 10409509
DOI: 10.1242/dev.126.16.3637

Abstract

Extracellular matrix (ECM) proteins play an important role in early cortical development, specifically in the formation of neural connections and in controlling the cyto-architecture of the central nervous system. F-spondin and Mindin are a family of matrix-attached adhesion molecules that share structural similarities and overlapping domains of expression. Genes for both proteins contain a thrombospondin type I repeat(s) at the C terminus and an FS1-FS2 (spondin) domain. Both the vertebrate F-spondin and the zebrafish mindins are expressed on the embryonic floor plate. In the current study we have cloned the rat homologue of mindin and studied its expression and activity together with F-spondin in the developing rodent brain. The two genes are abundantly expressed in the developing hippocampus. In vitro studies indicate that both F-spondin and Mindin promote adhesion and outgrowth of hippocampal embryonic neurons. We have also demonstrated that the two proteins bind to a putative receptor(s) expressed on both hippocampal and sensory neurons.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Cloning, Molecular
Drosophila
Drosophila Proteins
Extracellular Matrix Proteins / chemistry
Extracellular Matrix Proteins / genetics*
Extracellular Matrix Proteins / physiology
Gene Expression Regulation, Developmental*
Growth Substances*
Hippocampus / embryology*
Intercellular Signaling Peptides and Proteins
Membrane Proteins
Molecular Sequence Data
Neural Cell Adhesion Molecules / chemistry
Neural Cell Adhesion Molecules / genetics*
Neural Cell Adhesion Molecules / physiology
Neurons / physiology*
Organ Specificity
Peptides*
RNA, Messenger / analysis
RNA, Messenger / genetics
Rats
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Repetitive Sequences, Amino Acid
Sequence Alignment
Sequence Homology, Amino Acid
Transcription, Genetic
Zebrafish
Zebrafish Proteins*

Substances

Drosophila Proteins
Extracellular Matrix Proteins
Growth Substances
Intercellular Signaling Peptides and Proteins
Membrane Proteins
Neural Cell Adhesion Molecules
Peptides
RNA, Messenger
Recombinant Proteins
Spon1 protein, rat
Zebrafish Proteins
mindin
mspo protein, Drosophila
spon1a protein, zebrafish
spon2a protein, zebrafish
spon2b protein, zebrafish

Associated data

GENBANK/AF155196