Isolation and mapping of novel candidate genes for retinal disorders using suppression subtractive hybridization

Genomics. 1999 Jun 15;58(3):240-9. doi: 10.1006/geno.1999.5823.

Abstract

We have constructed human cDNA libraries enriched for retina- and retinal pigment epithelium (RPE)/choroid-specific cDNAs through suppression subtractive hybridization. The sequence of 314 cDNAs from the retina enriched library and 126 cDNAs from the RPE/choroid enriched library was analyzed. Based on the absence of a database match, 25% of the retina cDNA clones and 16% of the RPE/choroid cDNA clones are novel cDNAs. The expression profiles of 86 retina and 21 RPE/choroid cDNAs were determined by a semiquantitative reverse transcription polymerase chain reaction technique. Thirty-three cDNAs were expressed exclusively or most prominently in retina or RPE/choroid. These cDNAs were mapped in the human genome by radiation hybrid mapping. Eleven cDNAs colocalized with loci involved in retinal disorders. One cDNA mapped in a 1.5-megabase critical region for autosomal recessive retinitis pigmentosa (RP12). Another cDNA was assigned to the 7.7-cM RP17 linkage interval. Seven cDNAs colocalized with four loci involved in Bardet-Biedl syndrome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Choroid / metabolism
  • Chromosome Mapping
  • Cricetinae
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification*
  • Female
  • Gene Expression Regulation
  • Gene Library
  • Genes / genetics
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Nucleic Acid Hybridization / methods
  • Pigment Epithelium of Eye / metabolism
  • Retinal Diseases / genetics*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary

Associated data

  • GENBANK/AI444803
  • GENBANK/AI444804
  • GENBANK/AI444805
  • GENBANK/AI444806
  • GENBANK/AI444807
  • GENBANK/AI444808
  • GENBANK/AI444809
  • GENBANK/AI444810
  • GENBANK/AI444811
  • GENBANK/AI444812
  • GENBANK/AI444813
  • GENBANK/AI444814
  • GENBANK/AI444815
  • GENBANK/AI444816
  • GENBANK/AI444817
  • GENBANK/AI444818
  • GENBANK/AI444819
  • GENBANK/AI444820
  • GENBANK/AI444821
  • GENBANK/AI444822
  • GENBANK/AI444823
  • GENBANK/AI444824
  • GENBANK/AI444825
  • GENBANK/AI444826
  • GENBANK/AI444827
  • GENBANK/AI444828
  • GENBANK/AI444829
  • GENBANK/AI444830
  • GENBANK/AI444831
  • GENBANK/AI444832