Evolutionarily conserved, alternative splicing of reelin during brain development

Exp Neurol. 1999 Apr;156(2):229-38. doi: 10.1006/exnr.1999.7019.

Abstract

Reelin is the protein defective in reeler mutant mice and plays a pivotal role in brain development. However, some uncertainties remain about the relationship between reelin and the reeler phenotype. It is generally believed that reelin, secreted by specific neuronal types such as Cajal-Retzius cells, acts at short distance via the extracellular matrix on target neurons, the response of which requires the Dab1 gene product. However, the pattern of reelin expression in some structures such as olfactory bulb, retina, and spinal cord suggests that the protein might be endowed with different functions. In the present study, we identify two uncommon, evolutionarily conserved splicing events in the 3' part of the transcript that result in different forms of the protein. First, a 6-nucleotide, brain-specific microexon is skipped in about 10% of reelin RNA. In addition, an alternative polyadenylation event involving 10-25% of reelin mRNA results in secretion of a truncated protein lacking the terminal, highly basic stretch. This alternative reelin is generally expressed in the same cells as the major form, but is almost undetectable in retina and spinal cord. Both alternative splicing events are present in mouse, rat, and man, suggesting that the corresponding reelin forms are functionally important.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brain / cytology
  • Brain / embryology*
  • Brain / metabolism
  • COS Cells
  • Cell Adhesion Molecules, Neuronal / biosynthesis
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Cloning, Molecular
  • Exons / genetics
  • Extracellular Matrix Proteins / biosynthesis
  • Extracellular Matrix Proteins / genetics*
  • Eye Proteins / biosynthesis
  • Eye Proteins / genetics
  • Fetal Proteins / biosynthesis
  • Fetal Proteins / genetics
  • Gene Expression Regulation, Developmental*
  • Genes
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Neurons / metabolism
  • RNA Splicing*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Reelin Protein
  • Retina / cytology
  • Retina / embryology
  • Retina / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serine Endopeptidases
  • Spinal Cord / cytology
  • Spinal Cord / embryology
  • Spinal Cord / metabolism

Substances

  • Cell Adhesion Molecules, Neuronal
  • Extracellular Matrix Proteins
  • Eye Proteins
  • Fetal Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Reelin Protein
  • Reln protein, rat
  • RELN protein, human
  • Reln protein, mouse
  • Serine Endopeptidases