Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1alpha and -2alpha of bovine arterial endothelial cells

Biochim Biophys Acta. 1999 May 14;1445(2):237-43. doi: 10.1016/s0167-4781(99)00048-2.

Abstract

Hypoxia-inducible factor (HIF)-1alpha and -2alpha are two basic helix-loop-helix/PAS domain transcriptional factors that mediate hypoxia-induced gene expression. We found that bovine arterial endothelial cells (BAEC) expressed both HIF-1alpha and -2alpha by RT-PCR and then isolated cDNAs encoding these two transcriptional factors of BAEC. The deduced amino acid sequences of both HIF-1alpha and -2alpha showed high homologies among mammalian species. Northern blot analysis indicated that the mRNAs for HIF-1alpha and -2alpha from BAEC showed a size of approx. 5.5 and 6.2 kilobases, respectively and that both mRNAs were constitutively expressed and not induced by hypoxia in BAEC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cattle
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / chemistry*
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins*
  • Endothelium, Vascular / metabolism*
  • Gene Expression Regulation
  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Sequence Alignment
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism*
  • Transcription Factors*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA, Complementary
  • DNA-Binding Proteins
  • Hypoxia-Inducible Factor 1
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • Nuclear Proteins
  • Trans-Activators
  • Transcription Factors
  • endothelial PAS domain-containing protein 1

Associated data

  • GENBANK/AB018398
  • GENBANK/AB018399