Neem oil-water emulsion was used in mosquito breeding habitats to find out its larvicidal effect on immatures of different mosquito species. Application of 5% neem oil-water emulsion @ 50 ml/sq m in pools and @ 100 ml/sq m in tanks resulted in 100% reduction of III and IV instar larvae of An. stephensi after 24 h while, against Cx. quinquefasciatus it was 51.6 and 91.2% reduction in the larval density after Day 1 and 14 respectively. Similarly, application of 10% emulsion in desert coolers against Aedes aegypti @ 40 to 80 ml per cooler resulted in complete inhibition of pupal production.