Activation of somatostatin receptor II expression by transcription factors MIBP1 and SEF-2 in the murine brain

Mol Cell Biol. 1999 May;19(5):3736-47. doi: 10.1128/MCB.19.5.3736.

Abstract

Somatostatin receptor type II expression in the mammalian brain displays a spatially and temporally very restricted pattern. In an investigation of the molecular mechanisms controlling these patterns, we have recently shown that binding of the transcription factor SEF-2 to a novel initiator element in the SSTR-2 promoter is essential for SSTR-2 gene expression. Further characterization of the promoter identified a species-conserved TC-rich enhancer element. By screening a mouse brain cDNA expression library, we cloned a cDNA encoding the transcription factor MIBP1. MIBP1 interacts specifically with both the TC box in the SSTR-2 promoter and with the SEF-2 initiator-binding protein to enhance transcription from the basal SSTR-2 promoter. We then investigated SSTR-2, SEF-2, and MIBP1 mRNA expression patterns in the developing and adult murine brain by Northern blotting and in situ hybridization. While SEF-2 is widely expressed in many neuronal and nonneuronal tissues, MIBP1 expression overlapped precisely with expression of SSTR-2 in the frontal cortex and hippocampus. In summary, our data for the first time define a regulatory role for the transcription factor MIBP1 in mediating spatially and temporally regulated SSTR-2 expression in the brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Brain / embryology
  • Brain / metabolism*
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Embryonic and Fetal Development
  • Gene Expression Regulation, Developmental / genetics
  • Gene Library
  • Genes, Reporter / genetics
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / metabolism
  • Receptors, Somatostatin / genetics*
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • TCF Transcription Factors
  • Transcription Factor 4
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcriptional Activation / genetics*

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Hivep2 protein, mouse
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, Somatostatin
  • Recombinant Proteins
  • TCF Transcription Factors
  • Tcf4 protein, mouse
  • Tcf4 protein, rat
  • Transcription Factor 4
  • Transcription Factors
  • HIVEP2 protein, human
  • somatostatin receptor 2

Associated data

  • GENBANK/Y15907