Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits

Mol Cell. 1999 Feb;3(2):247-53. doi: 10.1016/s1097-2765(00)80315-9.

Abstract

Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / chemistry*
  • Actins / physiology
  • Adenosine Triphosphate / physiology
  • Cell-Free System
  • Chromatin / metabolism
  • Chromatin / ultrastructure*
  • Chromosomal Proteins, Non-Histone*
  • DNA Helicases
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / physiology
  • Drosophila Proteins*
  • Evolution, Molecular
  • Humans
  • Macromolecular Substances
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / physiology
  • Nucleosomes / metabolism
  • Nucleosomes / ultrastructure*
  • RNA-Binding Proteins*
  • Recombinant Fusion Proteins / chemistry
  • Ribonucleoprotein, U1 Small Nuclear / chemistry*
  • Ribonucleoprotein, U1 Small Nuclear / physiology
  • Transcription Factors / chemistry*
  • Transcription Factors / physiology

Substances

  • ACTL6A protein, human
  • Actins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Macromolecular Substances
  • Nuclear Proteins
  • Nucleosomes
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Ribonucleoprotein, U1 Small Nuclear
  • SMARCA2 protein, human
  • SMARCC1 protein, human
  • SMARCC2 protein, human
  • SMARCD1 protein, human
  • Transcription Factors
  • snf protein, Drosophila
  • Adenosine Triphosphate
  • SMARCA4 protein, human
  • DNA Helicases