Green fluorescent protein as a reporter of transcription and protein localization in fungi

B Cormack

doi:10.1016/s1369-5274(98)80057-x

Green fluorescent protein as a reporter of transcription and protein localization in fungi

Curr Opin Microbiol. 1998 Aug;1(4):406-10. doi: 10.1016/s1369-5274(98)80057-x.

Author

B Cormack¹

Affiliation

¹ Department of Molecular Biology, Johns Hopkins Medical School, Preclinical Teaching Building 522, 725 North Wolfe Street, Baltimore MD 21205-2185, USA. bcormack@bs.jhmi.edu

PMID: 10066516
DOI: 10.1016/s1369-5274(98)80057-x

Abstract

Green fluorescent protein (GFP) is a versatile and powerful tool for analysis of diverse biological processes. The recent development of GFP variants with altered spectral properties and altered codon composition has allowed efficient expression of GFP in a number of fungal species. GFP has been successfully used to analyze transcription regulation as well as protein and organelle localization, and promises to give an unprecedented view into the dynamic subcellular processes that shape the fungal cell.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell Compartmentation*
Fungal Proteins / genetics
Fungal Proteins / isolation & purification
Fungi / genetics*
Genes, Reporter*
Green Fluorescent Proteins
Indicators and Reagents
Luminescent Proteins / genetics*
Luminescent Proteins / isolation & purification
Saccharomyces cerevisiae / genetics
Schizosaccharomyces / genetics
Transcription, Genetic*

Substances

Fungal Proteins
Indicators and Reagents
Luminescent Proteins
Green Fluorescent Proteins