LOCUS NP_999460 1205 aa linear MAM 22-OCT-2008
DEFINITION nitric oxide synthase 3 (endothelial cell) [Sus scrofa].
ACCESSION NP_999460
VERSION NP_999460.1 GI:47523660
DBSOURCE REFSEQ: accession NM_214295.1
KEYWORDS .
SOURCE Sus scrofa (pig)
ORGANISM Sus scrofa
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
Sus.
REFERENCE 1 (residues 1 to 1205)
AUTHORS Musicki,B., Liu,T., Strong,T., Jin,L., Laughlin,M.H., Turk,J.R. and
Burnett,A.L.
TITLE Low-fat diet and exercise preserve eNOS regulation and endothelial
function in the penis of early atherosclerotic pigs: a molecular
analysis
JOURNAL J Sex Med 5 (3), 552-561 (2008)
PUBMED 18194184
REMARK GeneRIF: Low-fat diet and chronic exercise preserve endothelial
function in the pig penis by sustaining active eNOS in its dimeric
form and by limiting eNOS interaction with its negative regulator
caveolin-1.
REFERENCE 2 (residues 1 to 1205)
AUTHORS Shontz,K.M., Zhou,B., Yu,C.Y. and Su,B.Y.
TITLE Cloning and functional analysis of the swine eNOS promoter
JOURNAL DNA Seq. 19 (1), 62-67 (2008)
PUBMED 17852356
REMARK GeneRIF: Analysis of the 2.1 kb 5' flanking region reveals that the
swine eNOS promoter is, like its counterparts in human and other
species, a TATA-less promoter; the proximal region from -227 to -82
is necessary for eNOS basal promoter activity.
REFERENCE 3 (residues 1 to 1205)
AUTHORS Newcomer,S.C., Taylor,J.C., McAllister,R.M. and Laughlin,M.H.
TITLE Effects of chronic nitric oxide synthase inhibition on
endothelium-dependent and -independent relaxation in arteries that
perfuse skeletal muscle of swine
JOURNAL Endothelium 15 (1), 17-31 (2008)
PUBMED 18568942
REMARK GeneRIF: Effects of chronic NOS3 inhibition on
endothelium-dependent and -independent relaxation in arteries that
perfuse skeletal muscle of swine are reported.
REFERENCE 4 (residues 1 to 1205)
AUTHORS Lee,M.Y., Tse,H.F., Siu,C.W., Zhu,S.G., Man,R.Y. and Vanhoutte,P.M.
TITLE Genomic changes in regenerated porcine coronary arterial
endothelial cells
JOURNAL Arterioscler. Thromb. Vasc. Biol. 27 (11), 2443-2449 (2007)
PUBMED 17942849
REMARK GeneRIF: regenerated coronary endothelial cells exhibit
downregulation of eNOS
Erratum:[Arterioscler Thromb Vasc Biol. 2008 Feb;28(2):e13]
REFERENCE 5 (residues 1 to 1205)
AUTHORS Andronowska,A. and Chrusciel,M.
TITLE Expression and cellular distribution of NADPH-diaphorase and nitric
oxide synthases in the porcine uterus during early pregnancy
JOURNAL Folia Histochem. Cytobiol. 45 (4), 375-380 (2007)
PUBMED 18165177
REMARK GeneRIF: Immunoreactivity of eNOS was similar to NADPH-d staining.
Clear iNOS immunoreactivity was detected in the luminal epithelium,
endometrial stroma and individual endometrial glands.
REFERENCE 6 (residues 1 to 1205)
AUTHORS Fu,W., Chai,H., Yao,Q. and Chen,C.
TITLE Effects of HIV protease inhibitor ritonavir on vasomotor function
and endothelial nitric oxide synthase expression
JOURNAL J. Acquir. Immune Defic. Syndr. 39 (2), 152-158 (2005)
PUBMED 15905730
REMARK GeneRIF: Ritonavir significantly impairs vasomotor function and
reduces eNOS expression in porcine coronary artery endothelial
cells.
REFERENCE 7 (residues 1 to 1205)
AUTHORS Anter,E., Thomas,S.R., Schulz,E., Shapira,O.M., Vita,J.A. and
Keaney,J.F. Jr.
TITLE Activation of endothelial nitric-oxide synthase by the p38 MAPK in
response to black tea polyphenols
JOURNAL J. Biol. Chem. 279 (45), 46637-46643 (2004)
PUBMED 15333638
REMARK GeneRIF: the p38 MAPK is an upstream component of Akt-mediated eNOS
activation
REFERENCE 8 (residues 1 to 1205)
AUTHORS Barkoudah,E., Jaggar,J.H. and Leffler,C.W.
TITLE The permissive role of endothelial NO in CO-induced cerebrovascular
dilation
JOURNAL Am. J. Physiol. Heart Circ. Physiol. 287 (4), H1459-H1465 (2004)
PUBMED 15191891
REMARK GeneRIF: endothelium-derived NO stimulates guanylyl cyclase in
vascular smooth muscle cells and, thereby, permits CO to cause
dilation by activating K(Ca) channels
REFERENCE 9 (residues 1 to 1205)
AUTHORS Hu,H., Xin,M., Belayev,L.L., Zhang,J., Block,E.R. and Patel,J.M.
TITLE Autoinhibitory domain fragment of endothelial NOS enhances
pulmonary artery vasorelaxation by the NO-cGMP pathway
JOURNAL Am. J. Physiol. Lung Cell Mol. Physiol. 286 (5), L1066-L1074 (2004)
PUBMED 14729513
REMARK GeneRIF: The inhibitory domain of eNOs stimulates vasodilation of
the pulmonary artery.
REFERENCE 10 (residues 1 to 1205)
AUTHORS Zhang,J., Patel,J.M. and Block,E.R.
TITLE Molecular cloning, characterization and expression of a nitric
oxide synthase from porcine pulmonary artery endothelial cells
JOURNAL Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 116 (4), 485-491
(1997)
PUBMED 9149402
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from U59924.1.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
FEATURES Location/Qualifiers
source 1..1205
/organism="Sus scrofa"
/db_xref="taxon:9823"
Protein 1..1205
/product="nitric oxide synthase 3 (endothelial cell)"
/EC_number="1.14.13.39"
/function="this enzyme produces nitric oxide (NO) which
accounts for the biologic activity of endothelium-derived
relaxing factor (EDRF); important in regulation of
vasomotor tone and blood flow by inhibiting smooth muscle
contraction and platelet"
/calculated_mol_wt=133275
CDS 1..1205
/gene="NOS3"
/gene_synonym="eNOS"
/coded_by="NM_214295.1:7..3624"
/db_xref="GeneID:397557"
ORIGIN
1 mgnlksvgqe pgppcglglg lglglcgkqg patpapepsr apapatphap ehspapnspt
61 ltrppegpkf prvknwevgs itydtlcaqs qqdgpctprr clgslvlprk lqsrpspgpp
121 paeqllsqar dfinqyyssi krsgsqahee rlqeveaeva ttgtyhlges elvfgakqaw
181 rnaprcvgri qwgklqvfda rdcssaqemf tyicnhikya tnrgnlrsai tvfpqrtpgr
241 gdfriwnsql vryagyrqqd gsvrgdpanv eitelciqhg wtpgngrfdv lplllqapde
301 ppelfalppe lvlevplehp tlewfaalgl rwyalpavsn mlleigglef paapfsgwym
361 steigtrnlc dphryniled vavcmdldtr ttsslwkdka aveinlavlh syqlakvtiv
421 dhhaatasfm khleneqkar ggcpadwawi vppisgsltp vfhqemvnyv lspafryqpd
481 pwkgsaakgt giarkktfke vanavkisas lmatvmpkrv kasilyaset vraqsyaqql
541 grlfrkafdp rvlcmdeydv vslehetlvl vvtstfgngd ppengesfaa almemsgpyn
601 gsprpeqhrs ykirfnsvsc sdplvsswrr krkessntds agalgtlrfc vfglgsrayp
661 hfcafaravd trleelgger llqlgqgdel cgqeeafrgw aqaafqasce tfcvgedaka
721 aardifspkr swkrqryrls aqveglqllp glvhvhrrkm fqatvlsven lqsskstrat
781 ilvrldtegq eglqyqpgdh igicppnrtg lveallsrve dptpptesvg veqlekgspg
841 gpppswvrdp rlppytlrqa ltfflditsp psprllrvls tlaeepseqq eletlsqdpr
901 ryeewkwfrc ptllevleqf psvalptpll ltqlallqpr yysvssapst ypgeihptva
961 vlayrtqdgl gplhygvcst wlgqlkpgdp vpcfiraaps frlppdpslp cilvgpgtgi
1021 apfrgfwqer lhdieskglq papmtlvfgc rcsqldhlyr devqdaqqrg vfgrvltafs
1081 repdspktyv qdilrtelaa evhrvlcler ghmfvcgdvt matsvlqtvq rilategnme
1141 ldeagdvigv lrdqqryhed ifgltlrtqe vtsrirtqsf slqerhlrga vpwtfdppgp
1201 dtpgp
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