LOCUS NP_862821 118 aa linear PRI 22-OCT-2008
DEFINITION ubiquitin-conjugating enzyme E2D 2 isoform 2 [Homo sapiens].
ACCESSION NP_862821
VERSION NP_862821.1 GI:33188456
DBSOURCE REFSEQ: accession NM_181838.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 118)
AUTHORS Guinn,B.A., Bland,E.A., Lodi,U., Liggins,A.P., Tobal,K.,
Petters,S., Wells,J.W., Banham,A.H. and Mufti,G.J.
TITLE Humoral detection of leukaemia-associated antigens in presentation
acute myeloid leukaemia
JOURNAL Biochem. Biophys. Res. Commun. 335 (4), 1293-1304 (2005)
PUBMED 16112646
REFERENCE 2 (residues 1 to 118)
AUTHORS Saville,M.K., Sparks,A., Xirodimas,D.P., Wardrop,J.,
Stevenson,L.F., Bourdon,J.C., Woods,Y.L. and Lane,D.P.
TITLE Regulation of p53 by the ubiquitin-conjugating enzymes UbcH5B/C in
vivo
JOURNAL J. Biol. Chem. 279 (40), 42169-42181 (2004)
PUBMED 15280377
REMARK GeneRIF: UbcH5B/C are E2s for Mdm2, which contribute to the
maintenance of low levels of p53 and Mdm2 in unstressed cells;
inhibition of p53 ubiquitination and degradation by targeting
UbcH5B/C is not sufficient to up-regulate p53 transcriptional
activity.
REFERENCE 3 (residues 1 to 118)
AUTHORS Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
TITLE Functional proteomics mapping of a human signaling pathway
JOURNAL Genome Res. 14 (7), 1324-1332 (2004)
PUBMED 15231748
REFERENCE 4 (residues 1 to 118)
AUTHORS Winkler,G.S., Albert,T.K., Dominguez,C., Legtenberg,Y.I.,
Boelens,R. and Timmers,H.T.
TITLE An altered-specificity ubiquitin-conjugating
enzyme/ubiquitin-protein ligase pair
JOURNAL J. Mol. Biol. 337 (1), 157-165 (2004)
PUBMED 15001359
REMARK GeneRIF: Data show that binding of the CNOT4 RING finger to the
ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.
REFERENCE 5 (residues 1 to 118)
AUTHORS Lehner,B., Semple,J.I., Brown,S.E., Counsell,D., Campbell,R.D. and
Sanderson,C.M.
TITLE Analysis of a high-throughput yeast two-hybrid system and its use
to predict the function of intracellular proteins encoded within
the human MHC class III region
JOURNAL Genomics 83 (1), 153-167 (2004)
PUBMED 14667819
REFERENCE 6 (residues 1 to 118)
AUTHORS Anan,T., Nagata,Y., Koga,H., Honda,Y., Yabuki,N., Miyamoto,C.,
Kuwano,A., Matsuda,I., Endo,F., Saya,H. and Nakao,M.
TITLE Human ubiquitin-protein ligase Nedd4: expression, subcellular
localization and selective interaction with ubiquitin-conjugating
enzymes
JOURNAL Genes Cells 3 (11), 751-763 (1998)
PUBMED 9990509
REFERENCE 7 (residues 1 to 118)
AUTHORS Hatakeyama,S., Jensen,J.P. and Weissman,A.M.
TITLE Subcellular localization and ubiquitin-conjugating enzyme (E2)
interactions of mammalian HECT family ubiquitin protein ligases
JOURNAL J. Biol. Chem. 272 (24), 15085-15092 (1997)
PUBMED 9182527
REFERENCE 8 (residues 1 to 118)
AUTHORS Jensen,J.P., Bates,P.W., Yang,M., Vierstra,R.D. and Weissman,A.M.
TITLE Identification of a family of closely related human ubiquitin
conjugating enzymes
JOURNAL J. Biol. Chem. 270 (51), 30408-30414 (1995)
PUBMED 8530467
REFERENCE 9 (residues 1 to 118)
AUTHORS Rolfe,M., Beer-Romero,P., Glass,S., Eckstein,J., Berdo,I.,
Theodoras,A., Pagano,M. and Draetta,G.
TITLE Reconstitution of p53-ubiquitinylation reactions from purified
components: the role of human ubiquitin-conjugating enzyme UBC4 and
E6-associated protein (E6AP)
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 92 (8), 3264-3268 (1995)
PUBMED 7724550
REFERENCE 10 (residues 1 to 118)
AUTHORS Scheffner,M., Huibregtse,J.M. and Howley,P.M.
TITLE Identification of a human ubiquitin-conjugating enzyme that
mediates the E6-AP-dependent ubiquitination of p53
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 91 (19), 8797-8801 (1994)
PUBMED 8090726
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from U39317.1, AF317220.1 and
BC048426.1.
Summary: The modification of proteins with ubiquitin is an
important cellular mechanism for targeting abnormal or short-lived
proteins for degradation. Ubiquitination involves at least three
classes of enzymes: ubiquitin-activating enzymes, or E1s,
ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
ligases, or E3s. This gene encodes a member of the E2
ubiquitin-conjugating enzyme family. This enzyme functions in the
ubiquitination of the tumor-suppressor protein p53, which is
induced by an E3 ubiquitin-protein ligase. Two alternatively
spliced transcript variants have been found for this gene and they
encode distinct isoforms. [provided by RefSeq].
Transcript Variant: This variant (2) has an additional exon in the
5' region, and uses a downstream in-frame start codon, as compared
to variant 1. Isoform 2 thus has a shorter N-terminus, as compared
to isoform 1.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
FEATURES Location/Qualifiers
source 1..118
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="5"
/map="5q31.2"
Protein 1..118
/product="ubiquitin-conjugating enzyme E2D 2 isoform 2"
/EC_number="6.3.2.19"
/note="ubiquitin-conjugating enzyme E2D 2 (homologous to
yeast UBC4/5); ubiquitin carrier protein;
ubiquitin-conjugating enzyme E2-17 kDa 2;
ubiquitin-conjugating enzyme E2 D2"
/calculated_mol_wt=13504
CDS 1..118
/gene="UBE2D2"
/gene_synonym="UBC4"
/gene_synonym="PUBC1"
/gene_synonym="UBC4/5"
/gene_synonym="UBCH5B"
/gene_synonym="E2(17)KB2"
/coded_by="NM_181838.1:878..1234"
/note="isoform 2 is encoded by transcript variant 2"
/db_xref="GeneID:7322"
/db_xref="HGNC:12475"
/db_xref="MIM:602962"
ORIGIN
1 mfhwqatimg pndspyqggv ffltihfptd ypfkppkvaf ttriyhpnin sngsicldil
61 rsqwspalti skvllsicsl lcdpnpddpl vpeiariykt drekynriar ewtqkyam
//