LOCUS NP_777071 145 aa linear MAM 22-OCT-2008
DEFINITION phospholipase A2, group IB [Bos taurus].
ACCESSION NP_777071
VERSION NP_777071.1 GI:27807165
DBSOURCE REFSEQ: accession NM_174646.2
KEYWORDS .
SOURCE Bos taurus (cattle)
ORGANISM Bos taurus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
Pecora; Bovidae; Bovinae; Bos.
REFERENCE 1 (residues 1 to 145)
AUTHORS Sekar,K., Yogavel,M., Kanaujia,S.P., Sharma,A., Velmurugan,D.,
Poi,M.J., Dauter,Z. and Tsai,M.D.
TITLE Suggestive evidence for the involvement of the second calcium and
surface loop in interfacial binding: monoclinic and trigonal
crystal structures of a quadruple mutant of phospholipase A2
JOURNAL Acta Crystallogr. D Biol. Crystallogr. 62 (PT 7), 717-724 (2006)
PUBMED 16790927
REMARK GeneRIF: The quadruple mutant of phospholipase A2 has monoclinic or
trigonal crystal structures and results suggested evidence for the
involvement of the second calcium and surface loop in interfacial
binding.
REFERENCE 2 (residues 1 to 145)
AUTHORS Sekar,K., Gayathri,D., Velmurugan,D., Jeyakanthan,J., Yamane,T.,
Poi,M.J. and Tsai,M.D.
TITLE Third calcium ion found in an inhibitor-bound phospholipase A2
JOURNAL Acta Crystallogr. D Biol. Crystallogr. 62 (PT 4), 392-397 (2006)
PUBMED 16552140
REMARK GeneRIF: The crystal structure of the triple mutant (K53,56,121M)
of bovine pancreatic phospholipase A2 complexed with an organic
molecule, p-methoxybenzoic acid (anisic acid), is reported.
REFERENCE 3 (residues 1 to 145)
AUTHORS Sekar,K., Rajakannan,V., Gayathri,D., Velmurugan,D., Poi,M.J.,
Dauter,M., Dauter,Z. and Tsai,M.D.
TITLE Atomic resolution (0.97 A) structure of the triple mutant
(K53,56,121M) of bovine pancreatic phospholipase A2
JOURNAL Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 (PT 1),
3-7 (2005)
PUBMED 16508077
REMARK GeneRIF: The final protein model consists of 123 amino-acid
residues, two calcium ions, one chloride ion, 243 water molecules
and six 2-methyl-2,4-pentanediol molecules.
REFERENCE 4 (residues 1 to 145)
AUTHORS Poi,M.J., Tomaszewski,J.W., Yuan,C., Dunlap,C.A., Andersen,N.H.,
Gelb,M.H. and Tsai,M.D.
TITLE A low-barrier hydrogen bond between histidine of secreted
phospholipase A2 and a transition state analog inhibitor
JOURNAL J. Mol. Biol. 329 (5), 997-1009 (2003)
PUBMED 12798689
REMARK GeneRIF: NMR characterization of a unique low-barrier hydrogen bond
between an active site residue from the enzyme and a bound
inhibitor: the complex between secreted phospholipase A(2)
(sPLA(2), from bovine pancreas) and a transition-state analog
inhibitor HK32
REFERENCE 5 (residues 1 to 145)
AUTHORS Rajakannan,V., Yogavel,M., Poi,M.J., Jeyaprakash,A.A.,
Jeyakanthan,J., Velmurugan,D., Tsai,M.D. and Sekar,K.
TITLE Observation of additional calcium ion in the crystal structure of
the triple mutant K56,120,121M of bovine pancreatic phospholipase
A2
JOURNAL J. Mol. Biol. 324 (4), 755-762 (2002)
PUBMED 12460575
REMARK GeneRIF: crystal structure of the triple mutant pancreatic
phospholipase A2 with calcium.
REFERENCE 6 (residues 1 to 145)
AUTHORS Tanaka,T., Kimura,S. and Ota,Y.
TITLE Sequence of a cDNA coding for bovine pancreatic phospholipase A2
JOURNAL Nucleic Acids Res. 15 (7), 3178 (1987)
PUBMED 3562249
REMARK Erratum:[Nucleic Acids Res 1987 Jun 11;15(11):4704]
REFERENCE 7 (residues 1 to 145)
AUTHORS Dijkstra,B.W., Kalk,K.H., Hol,W.G. and Drenth,J.
TITLE Structure of bovine pancreatic phospholipase A2 at 1.7A resolution
JOURNAL J. Mol. Biol. 147 (1), 97-123 (1981)
PUBMED 7265241
REFERENCE 8 (residues 1 to 145)
AUTHORS Dijkstra,B.W., Drenth,J. and Kalk,K.H.
TITLE Active site and catalytic mechanism of phospholipase A2
JOURNAL Nature 289 (5798), 604-606 (1981)
PUBMED 7464926
REFERENCE 9 (residues 1 to 145)
AUTHORS Dijkstra,B.W., Drenth,J., Kalk,K.H. and Vandermaelen,P.J.
TITLE Three-dimensional structure and disulfide bond connections in
bovine pancreatic phospholipase A2
JOURNAL J. Mol. Biol. 124 (1), 53-60 (1978)
PUBMED 712836
REFERENCE 10 (residues 1 to 145)
AUTHORS Fleer,E.A., Verheij,H.M. and De Haas,G.H.
TITLE The primary structure of bovine pancreatic phospholipase A2
JOURNAL Eur. J. Biochem. 82 (1), 261-269 (1978)
PUBMED 620674
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from Y00120.1.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
FEATURES Location/Qualifiers
source 1..145
/organism="Bos taurus"
/db_xref="taxon:9913"
/chromosome="17"
/map="17"
Protein 1..145
/product="phospholipase A2, group IB"
/EC_number="3.1.1.4"
sig_peptide 1..15
/note="put. signal peptide (AA -15 to -1)"
/calculated_mol_wt=1498
mat_peptide 23..145
/product="mature phospholipase A2 (AA 8-130)"
/calculated_mol_wt=13810
CDS 1..145
/gene="PLA2G1B"
/coded_by="NM_174646.2:18..455"
/db_xref="GeneID:282457"
ORIGIN
1 mrllvlaall tvgagqagln sralwqfngm ikckipssep lldfnnygcy cglggsgtpv
61 ddldrccqth dncykqakkl dsckvlvdnp ytnnysyscs nneitcssen naceaficnc
121 drnaaicfsk vpynkehknl dkkkc
//