LOCUS NP_009117 2442 aa linear PRI 10-FEB-2008
DEFINITION centrosomal protein 2 isoform 1 [Homo sapiens].
ACCESSION NP_009117
VERSION NP_009117.2 GI:21735548
DBSOURCE REFSEQ: accession NM_007186.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 2442)
AUTHORS Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
TITLE Proteomic, functional, and domain-based analysis of in vivo 14-3-3
binding proteins involved in cytoskeletal regulation and cellular
organization
JOURNAL Curr. Biol. 14 (16), 1436-1450 (2004)
PUBMED 15324660
REFERENCE 2 (residues 1 to 2442)
AUTHORS Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
TITLE Functional proteomics mapping of a human signaling pathway
JOURNAL Genome Res. 14 (7), 1324-1332 (2004)
PUBMED 15231748
REFERENCE 3 (residues 1 to 2442)
AUTHORS Faragher,A.J. and Fry,A.M.
TITLE Nek2A kinase stimulates centrosome disjunction and is required for
formation of bipolar mitotic spindles
JOURNAL Mol. Biol. Cell 14 (7), 2876-2889 (2003)
PUBMED 12857871
REFERENCE 4 (residues 1 to 2442)
AUTHORS Mayor,T., Hacker,U., Stierhof,Y.D. and Nigg,E.A.
TITLE The mechanism regulating the dissociation of the centrosomal
protein C-Nap1 from mitotic spindle poles
JOURNAL J. Cell. Sci. 115 (PT 16), 3275-3284 (2002)
PUBMED 12140259
REMARK GeneRIF: Data show that the dissociation of C-Nap1 from mitotic
centrosomes is regulated by localized phosphorylation rather than
generalized proteolysis.
REFERENCE 5 (residues 1 to 2442)
AUTHORS Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
Rogers,J.
TITLE The DNA sequence and comparative analysis of human chromosome 20
JOURNAL Nature 414 (6866), 865-871 (2001)
PUBMED 11780052
REFERENCE 6 (residues 1 to 2442)
AUTHORS Hirsch,D.S., Pirone,D.M. and Burbelo,P.D.
TITLE A new family of Cdc42 effector proteins, CEPs, function in
fibroblast and epithelial cell shape changes
JOURNAL J. Biol. Chem. 276 (2), 875-883 (2001)
PUBMED 11035016
REFERENCE 7 (residues 1 to 2442)
AUTHORS Mayor,T., Stierhof,Y.D., Tanaka,K., Fry,A.M. and Nigg,E.A.
TITLE The centrosomal protein C-Nap1 is required for cell cycle-regulated
centrosome cohesion
JOURNAL J. Cell Biol. 151 (4), 837-846 (2000)
PUBMED 11076968
REFERENCE 8 (residues 1 to 2442)
AUTHORS Helps,N.R., Luo,X., Barker,H.M. and Cohen,P.T.
TITLE NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase
localized to centrosomes, is complexed to protein phosphatase 1
JOURNAL Biochem. J. 349 (PT 2), 509-518 (2000)
PUBMED 10880350
REFERENCE 9 (residues 1 to 2442)
AUTHORS Fry,A.M., Mayor,T., Meraldi,P., Stierhof,Y.D., Tanaka,K. and
Nigg,E.A.
TITLE C-Nap1, a novel centrosomal coiled-coil protein and candidate
substrate of the cell cycle-regulated protein kinase Nek2
JOURNAL J. Cell Biol. 141 (7), 1563-1574 (1998)
PUBMED 9647649
REFERENCE 10 (residues 1 to 2442)
AUTHORS Mack,G.J., Rees,J., Sandblom,O., Balczon,R., Fritzler,M.J. and
Rattner,J.B.
TITLE Autoantibodies to a group of centrosomal proteins in human
autoimmune sera reactive with the centrosome
JOURNAL Arthritis Rheum. 41 (3), 551-558 (1998)
PUBMED 9506584
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AF049105.1, AF022655.1 and
AL037087.2.
On Jul 12, 2002 this sequence version replaced gi:6005729.
Summary: This gene encodes a core centrosomal protein required for
centriole-centriole cohesion during interphase of the cell cycle.
The encoded protein dissociates from the centrosomes when parental
centrioles separate at the beginning of mitosis. The protein
associates with and is phosphorylated by NIMA-related kinase 2,
which is also associated with the centrosome. Alternately spliced
transcript variants encoding different isoforms have been
described.
Transcript Variant: This variant (1) represents the longer
transcript and encodes the longer isoform (1).
FEATURES Location/Qualifiers
source 1..2442
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="20"
/map="20q11.22-q12"
Protein 1..2442
/product="centrosomal protein 2 isoform 1"
/note="centrosome associated protein; centrosomal
Nek2-associated protein 1; centrosomal protein, 250-KD"
/calculated_mol_wt=281007
CDS 1..2442
/gene="CEP250"
/gene_synonym="CEP2"
/gene_synonym="C-NAP1"
/gene_synonym="MGC88542"
/coded_by="NM_007186.2:672..8000"
/note="isoform 1 is encoded by transcript variant 1"
/db_xref="CCDS:CCDS13255.1"
/db_xref="GeneID:11190"
/db_xref="HGNC:1859"
/db_xref="HPRD:09877"
/db_xref="MIM:609689"
ORIGIN
1 metrspglnn mkpqslqlvl eeqvlalqqq maenqaaswr klknsqeaqq rqatlvrklq
61 akvlqyrswc qelekrleat ggpipqrwen veepnldell vrleeeqqrc eslaevntql
121 rlhmekadvv nkalredvek ltvdwsrard elmrkesqwq meqeffkgyl kgehgrllsl
181 wrevvtfrrh flemksatdr dlmelkaehv rlsgslltcc lrltvgaqsr epngsgrmdg
241 repaqlllll aktqelekea hersqeliql ksqgdlekae lqdrvtelsa lltqsqkqne
301 dyekmikalr etveiletnh telmeheasl srnaqeekls lqqvikditq vmveegdnia
361 qgsghensle ldssifsqfd yqdadkaltl vrsvltrrrq avqdlrqqla gcqeavnllq
421 qqhdqweeeg kalrqrlqkl tgerdtlagq tvdlqgevds lskerellqk areelrqqle
481 vleqeawrlr rvnvelqlqg dsaqgqkeeq qeelhlavre rerlqemlmg leakqsesls
541 elitlreale sshlegellr qeqtevtaal araeqsiael sssentlkte vadlraaavk
601 lsalnealal dkvglnqqll qleeenqsvc srmeaaeqar nalqvdlaea ekrrealwek
661 nthleaqlqk aeeagaelqa dlrdiqeeke eiqkklsesr hqqeaattql eqlhqeakrq
721 eevlaravqe kealvrekaa levrlqaver drqdlaeqlq glssakelle sslfeaqqqn
781 svievtkgql evqiqtvtqa keviqgevrc lkleldters qaeqerdaaa rqlaqaeqeg
841 ktaleqqkaa hekevnqlre kwekerswhq qelakalesl erekmelemr lkeqqtemea
901 iqaqreeert qaesalcqmq letekervsl letllqtqke ladasqqler lrqdmkvqkl
961 keqettgilq tqlqeaqrel keaarqhrdd laalqeesss llqdkmdlqk qvedlksqlv
1021 aqddsqrlve qevqeklret qeynriqkel erekasltls lmekeqrllv lqeadsirqq
1081 elsalrqdmq eaqgeqkels aqmellrqev kekeadflaq eaqlleelea shiteqqlra
1141 slwaqeakaa qlqlrlrste sqlealaaeq qpgnqaqaqa qlaslysalq qalgsvcesr
1201 pelsgggdsa psvwglepdq ngarslfkrg plltalsaea vasalhklhq dlwktqqtrd
1261 vlrdqvqkle erltdteaek sqvhtelqdl qrqlsqnqee kskwegkqns leselmelhe
1321 tmaslqsrlr raelqrmeaq gerellqaak enltaqvehl qaavvearaq asaagileed
1381 lrtarsalkl kneevesere raqalqeqge lkvaqgkalq enlalltqtl aereeevetl
1441 rgqiqelekq remqkaalel lsldlkkrnq evdlqqeqiq elekcrsvle hlpmavqere
1501 qkltvqreqi relekdretq rnvlehqlle lekkdqmies qrgqvqdlkk qlvtleclal
1561 eleenhhkme cqqklikele gqretqrval thltldleer sqelqaqssq ihdleshstv
1621 larelqerdq evksqreqie elqrqkehlt qdlerrdqel mlqkeriqvl edqrtrqtki
1681 leedleqikl slrergrelt tqrqlmqera eegkgpskaq rgslehmkli lrdkekevec
1741 qqehihelqe lkdqleqqlq glhrkvgets lllsqreqei vvlqqqlqea reqgelkeqs
1801 lqsqldeaqr alaqrdqele alqqeqqqaq gqeervkeka dalqgaleqa hmtlkerhge
1861 lqdhkeqarr leeelavegr rvqaleevlg dlraesreqe kallalqqqc aeqaqeheve
1921 tralqdswlq aqavlkerdq elealraesq ssrhqeeaar araealqeal gkahaalqgk
1981 eqhlleqael srsleastat lqasldacqa hsrqleealr iqegeiqdqd lryqedvqql
2041 qqalaqrdee lrhqqereql lekslaqrvq enmiqekqnl gqereeeeir glhqsvrelq
2101 ltlaqkeqei lelretqqrn nlealphshk tspmeeqslk ldsleprlqr elerlqaalr
2161 qteareiewr ekaqdlalsl aqtkasvssl qevamflqas vlerdseqqr lqdeleltrr
2221 alekerlhsp gatstaelgs rgeqgvqlge vsgveaepsp dgmekqswrq rlehlqqava
2281 rleidrsrlq rhnvqlrstl eqvererrkl kreamraaqa gsleiskata ssptqqdgrg
2341 qknsdakcva elqkevvllq aqltlerkqk qdyitrsaqt srelaglhhs lshsllavaq
2401 apeatvleae trrldesltq sltspgpvll hpspsttqaa sr
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