LOCUS       NP_000937                420 aa            linear   PRI 11-MAY-2008
DEFINITION  DNA primase polypeptide 1 [Homo sapiens].
ACCESSION   NP_000937
VERSION     NP_000937.1  GI:4506051
DBSOURCE    REFSEQ: accession NM_000946.2
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 420)
  AUTHORS   Melle,C. and Nasheuer,H.P.
  TITLE     Physical and functional interactions of the tumor suppressor
            protein p53 and DNA polymerase alpha-primase
  JOURNAL   Nucleic Acids Res. 30 (7), 1493-1499 (2002)
   PUBMED   11917009
REFERENCE   2  (residues 1 to 420)
  AUTHORS   Bae,S.H., Bae,K.H., Kim,J.A. and Seo,Y.S.
  TITLE     RPA governs endonuclease switching during processing of Okazaki
            fragments in eukaryotes
  JOURNAL   Nature 412 (6845), 456-461 (2001)
   PUBMED   11473323
REFERENCE   3  (residues 1 to 420)
  AUTHORS   Arezi,B., Kirk,B.W., Copeland,W.C. and Kuchta,R.D.
  TITLE     Interactions of DNA with human DNA primase monitored with
            photoactivatable cross-linking agents: implications for the role of
            the p58 subunit
  JOURNAL   Biochemistry 38 (39), 12899-12907 (1999)
   PUBMED   10504261
REFERENCE   4  (residues 1 to 420)
  AUTHORS   Yotov,W.V., Hamel,H., Rivard,G.E., Champagne,M.A., Russo,P.A.,
            Leclerc,J.M., Bernstein,M.L. and Levy,E.
  TITLE     Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma
  JOURNAL   Genes Chromosomes Cancer 26 (1), 62-69 (1999)
   PUBMED   10441007
REFERENCE   5  (residues 1 to 420)
  AUTHORS   Podust,V.N., Tiwari,N., Stephan,S. and Fanning,E.
  TITLE     Replication factor C disengages from proliferating cell nuclear
            antigen (PCNA) upon sliding clamp formation, and PCNA itself
            tethers DNA polymerase delta to DNA
  JOURNAL   J. Biol. Chem. 273 (48), 31992-31999 (1998)
   PUBMED   9822671
REFERENCE   6  (residues 1 to 420)
  AUTHORS   Schneider,A., Smith,R.W., Kautz,A.R., Weisshart,K., Grosse,F. and
            Nasheuer,H.P.
  TITLE     Primase activity of human DNA polymerase alpha-primase. Divalent
            cations stabilize the enzyme activity of the p48 subunit
  JOURNAL   J. Biol. Chem. 273 (34), 21608-21615 (1998)
   PUBMED   9705292
REFERENCE   7  (residues 1 to 420)
  AUTHORS   Cloutier,S., Hamel,H., Champagne,M. and Yotov,W.V.
  TITLE     Mapping of the human DNA primase 1 (PRIM1) to chromosome 12q13
  JOURNAL   Genomics 43 (3), 398-401 (1997)
   PUBMED   9268648
REFERENCE   8  (residues 1 to 420)
  AUTHORS   Shiratori,A., Okumura,K., Nogami,M., Taguchi,H., Onozaki,T.,
            Inoue,T., Ando,T., Shibata,T., Izumi,M., Miyazawa,H. et al.
  TITLE     Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B)
            subunit genes of the human DNA primase to chromosome bands 1q44 and
            6p11.1-p12
  JOURNAL   Genomics 28 (2), 350-353 (1995)
   PUBMED   8530050
REFERENCE   9  (residues 1 to 420)
  AUTHORS   Stadlbauer,F., Brueckner,A., Rehfuess,C., Eckerskorn,C.,
            Lottspeich,F., Forster,V., Tseng,B.Y. and Nasheuer,H.P.
  TITLE     DNA replication in vitro by recombinant
            DNA-polymerase-alpha-primase
  JOURNAL   Eur. J. Biochem. 222 (3), 781-793 (1994)
   PUBMED   8026492
REFERENCE   10 (residues 1 to 420)
  AUTHORS   Wang,T.S., Hu,S.Z. and Korn,D.
  TITLE     DNA primase from KB cells. Characterization of a primase activity
            tightly associated with immunoaffinity-purified DNA
            polymerase-alpha
  JOURNAL   J. Biol. Chem. 259 (3), 1854-1865 (1984)
   PUBMED   6693436
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU167590.1, CD686660.1,
            BC005266.1 and AA761682.1.
            
            Summary: The replication of DNA in eukaryotic cells is carried out
            by a complex chromosomal replication apparatus, in which DNA
            polymerase alpha and primase are two key enzymatic components.
            Primase, which is a heterodimer of a small subunit and a large
            subunit, synthesizes small RNA primers for the Okazaki fragments
            made during discontinuous DNA replication. The protein encoded by
            this gene is the small, 49 kDa primase subunit.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13"
     Protein         1..420
                     /product="DNA primase polypeptide 1"
                     /EC_number="2.7.7.-"
                     /note="primase p49 subunit; DNA primase small subunit; DNA
                     primase subunit 48; DNA primase 1; primase polypeptide 1,
                     49kDa"
                     /calculated_mol_wt=49771
     CDS             1..420
                     /gene="PRIM1"
                     /gene_synonym="p49"
                     /gene_synonym="MGC12308"
                     /coded_by="NM_000946.2:65..1327"
                     /db_xref="GeneID:5557"
                     /db_xref="HGNC:9369"
                     /db_xref="HPRD:08899"
                     /db_xref="MIM:176635"
ORIGIN      
        1 metfdptelp ellklyyrrl fpysqyyrwl nyggviknyf qhrefsftlk ddiyiryqsf
       61 nnqsdlekem qkmnpykidi gavyshrpnq hntvklgafq aqekelvfdi dmtdyddvrr
      121 ccssadicpk cwtlmtmair iidralkedf gfkhrlwvys grrgvhcwvc desvrklssa
      181 vrsgiveyls lvkggqdvkk kvhlsekihp firksiniik kyfeeyalvn qdilenkesw
      241 dkilalvpet ihdelqqsfq kshnslqrwe hlkkvasryq nnikndkygp wleweimlqy
      301 cfprldinvs kginhllksp fsvhpktgri svpidlqkvd qfdpftvpti sficreldai
      361 stneeekeen eaesdvkhrt rdykktslap yvkvfehfle nldksrkgel lkksdlqkdf
//