LOCUS NP_000928 1970 aa linear PRI 12-OCT-2008
DEFINITION DNA directed RNA polymerase II polypeptide A [Homo sapiens].
ACCESSION NP_000928
VERSION NP_000928.1 GI:4505939
DBSOURCE REFSEQ: accession NM_000937.3
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 1970)
AUTHORS Chen,Y.S., Huang,W.H., Hong,S.Y., Tsay,Y.G. and Chen,P.J.
TITLE ERK1/2-mediated phosphorylation of small hepatitis delta antigen at
serine 177 enhances hepatitis delta virus antigenomic RNA
replication
JOURNAL J. Virol. 82 (19), 9345-9358 (2008)
PUBMED 18632853
REMARK GeneRIF: ERK1/2-mediated Ser-177 phosphorylation has a role in
modulating HDV antigenomic RNA replication, possibly through RNAPII
regulation
REFERENCE 2 (residues 1 to 1970)
AUTHORS Alm-Kristiansen,A.H., Saether,T., Matre,V., Gilfillan,S., Dahle,O.
and Gabrielsen,O.S.
TITLE FLASH acts as a co-activator of the transcription factor c-Myb and
localizes to active RNA polymerase II foci
JOURNAL Oncogene 27 (34), 4644-4656 (2008)
PUBMED 18408764
REMARK GeneRIF: c-Myb cooperates with FLASH in foci associated with active
RNA polymerase II, leading to enhancement of Myb-dependent gene
activation.
REFERENCE 3 (residues 1 to 1970)
AUTHORS Hirose,Y., Iwamoto,Y., Sakuraba,K., Yunokuchi,I., Harada,F. and
Ohkuma,Y.
TITLE Human phosphorylated CTD-interacting protein, PCIF1, negatively
modulates gene expression by RNA polymerase II
JOURNAL Biochem. Biophys. Res. Commun. 369 (2), 449-455 (2008)
PUBMED 18294453
REMARK GeneRIF: These data suggest that icariin exerts its potent
osteogenic effect through induction of Runx2 expression, production
of BMP-4 and activation of BMP signaling.
REFERENCE 4 (residues 1 to 1970)
AUTHORS West,S., Proudfoot,N.J. and Dye,M.J.
TITLE Molecular dissection of mammalian RNA polymerase II transcriptional
termination
JOURNAL Mol. Cell 29 (5), 600-610 (2008)
PUBMED 18342606
REMARK GeneRIF: Results describe transcript and template release in the
context of both natural and artificial RNA polymerase II terminator
sequences, including the timing and relationship between these
events.
REFERENCE 5 (residues 1 to 1970)
AUTHORS Chapman,R.D., Heidemann,M., Albert,T.K., Mailhammer,R., Flatley,A.,
Meisterernst,M., Kremmer,E. and Eick,D.
TITLE Transcribing RNA polymerase II is phosphorylated at CTD residue
serine-7
JOURNAL Science 318 (5857), 1780-1782 (2007)
PUBMED 18079404
REMARK GeneRIF: results indicate that restriction of serine-7 epitopes to
the Linker-proximal region limits carboxyl-terminal repeat domain
phosphorylation patterns and is a requirement for optimal gene
expression
REFERENCE 6 (sites)
AUTHORS Ficarro,S.B., Salomon,A.R., Brill,L.M., Mason,D.E.,
Stettler-Gill,M., Brock,A. and Peters,E.C.
TITLE Automated immobilized metal affinity chromatography/nano-liquid
chromatography/electrospray ionization mass spectrometry platform
for profiling protein phosphorylation sites
JOURNAL Rapid Commun. Mass Spectrom. 19 (1), 57-71 (2005)
PUBMED 15570572
REFERENCE 7 (sites)
AUTHORS Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
TITLE Large-scale characterization of HeLa cell nuclear phosphoproteins
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)
PUBMED 15302935
REFERENCE 8 (sites)
AUTHORS Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
Stettler-Gill,M. and Peters,E.C.
TITLE Robust phosphoproteomic profiling of tyrosine phosphorylation sites
from human T cells using immobilized metal affinity chromatography
and tandem mass spectrometry
JOURNAL Anal. Chem. 76 (10), 2763-2772 (2004)
PUBMED 15144186
REFERENCE 9 (residues 1 to 1970)
AUTHORS Barron-Casella,E. and Corden,J.L.
TITLE Conservation of the mammalian RNA polymerase II largest-subunit
C-terminal domain
JOURNAL J. Mol. Evol. 35 (5), 405-410 (1992)
PUBMED 1487824
REFERENCE 10 (residues 1 to 1970)
AUTHORS Kato,H., Sumimoto,H., Pognonec,P., Chen,C.H., Rosen,C.A. and
Roeder,R.G.
TITLE HIV-1 Tat acts as a processivity factor in vitro in conjunction
with cellular elongation factors
JOURNAL Genes Dev. 6 (4), 655-666 (1992)
PUBMED 1559613
REFERENCE 11 (residues 1 to 1970)
AUTHORS Wintzerith,M., Acker,J., Vicaire,S., Vigneron,M. and Kedinger,C.
TITLE Complete sequence of the human RNA polymerase II largest subunit
JOURNAL Nucleic Acids Res. 20 (4), 910 (1992)
PUBMED 1542581
REFERENCE 12 (residues 1 to 1970)
AUTHORS Jacob,G.A., Luse,S.W. and Luse,D.S.
TITLE Abortive initiation is increased only for the weakest members of a
set of down mutants of the adenovirus 2 major late promoter
JOURNAL J. Biol. Chem. 266 (33), 22537-22544 (1991)
PUBMED 1939271
REFERENCE 13 (residues 1 to 1970)
AUTHORS Smith,S.S., Kelly,K.H. and Jockusch,B.M.
TITLE Actin co-purifies with RNA polymerase II
JOURNAL Biochem. Biophys. Res. Commun. 86 (1), 161-166 (1979)
PUBMED 435297
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from BU739765.1,
X63564.1, AC113189.11, BQ891728.1, DA923649.1 and CV355924.1.
Summary: This gene encodes the largest subunit of RNA polymerase
II, the polymerase responsible for synthesizing messenger RNA in
eukaryotes. The product of this gene contains a carboxy terminal
domain composed of heptapeptide repeats that are essential for
polymerase activity. These repeats contain serine and threonine
residues that are phosphorylated in actively transcribing RNA
polymerase. In addition, this subunit, in combination with several
other polymerase subunits, forms the DNA binding domain of the
polymerase, a groove in which the DNA template is transcribed into
RNA. [provided by RefSeq].
Sequence Note: This RefSeq record was created from transcript and
genomic sequence data because no single transcript was available
for the full length of the gene. The extent of this transcript is
supported by transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
FEATURES Location/Qualifiers
source 1..1970
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="17"
/map="17p13.1"
Protein 1..1970
/product="DNA directed RNA polymerase II polypeptide A"
/EC_number="2.7.7.6"
/note="polymerase (RNA) II (DNA directed) polypeptide A
(220kD); DNA-directed RNA polymerase II largest subunit,
RNA polymerase II 220 kd subunit"
/calculated_mol_wt=217076
Site 1836
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[7]
Site 1878
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[6]
Site 1882
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[7]
Site 1896
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[7]
Site 1927
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[8]
Site 1929
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[8]
Site 1930
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[8]
Site 1934
/site_type="phosphorylation"
/experiment="experimental evidence, no additional details
recorded"
/citation=[7]
CDS 1..1970
/gene="POLR2A"
/gene_synonym="RPB1"
/gene_synonym="RPO2"
/gene_synonym="POLR2"
/gene_synonym="POLRA"
/gene_synonym="RPBh1"
/gene_synonym="RPOL2"
/gene_synonym="RpIILS"
/gene_synonym="hsRPB1"
/gene_synonym="hRPB220"
/gene_synonym="MGC75453"
/coded_by="NM_000937.3:235..6147"
/db_xref="CCDS:CCDS32548.1"
/db_xref="GeneID:5430"
/db_xref="HGNC:9187"
/db_xref="HPRD:08916"
/db_xref="MIM:180660"
ORIGIN
1 mhgggppsgd sacplrtikr vqfgvlspde lkrmsvtegg ikypettegg rpklgglmdp
61 rqgviertgr cqtcagnmte cpghfghiel akpvfhvgfl vktmkvlrcv cffcskllvd
121 snnpkikdil akskgqpkkr lthvydlckg kniceggeem dnkfgveqpe gdedltkekg
181 hggcgryqpr irrsglelya ewkhvnedsq ekkillsper vheifkrisd eecfvlgmep
241 ryarpewmiv tvlpvpplsv rpavvmqgsa rnqddlthkl adivkinnql rrneqngaaa
301 hviaedvkll qfhvatmvdn elpglpramq ksgrplkslk qrlkgkegrv rgnlmgkrvd
361 fsartvitpd pnlsidqvgv prsiaanmtf aeivtpfnid rlqelvrrgn sqypgakyii
421 rdngdridlr fhpkpsdlhl qtgykverhm cdgdivifnr qptlhkmsmm ghrvrilpws
481 tfrlnlsvtt pynadfdgde mnlhlpqsle traeiqelam vprmivtpqs nrpvmgivqd
541 tltavrkftk rdvflergev mnllmflstw dgkvpqpail kprplwtgkq ifsliipghi
601 ncirthsthp ddedsgpykh ispgdtkvvv engelimgil ckkslgtsag slvhisylem
661 ghditrlfys niqtvinnwl lieghtigig dsiadsktyq diqntikkak qdvievieka
721 hnneleptpg ntlrqtfenq vnrilndard ktgssaqksl seynnfksmv vsgakgskin
781 isqviavvgq qnvegkripf gfkhrtlphf ikddygpesr gfvensylag ltptefffha
841 mggreglidt avktaetgyi qrrliksmes vmvkydatvr nsinqvvqlr ygedglages
901 vefqnlatlk psnkafekkf rfdytneral rrtlqedlvk dvlsnahiqn elerefermr
961 edrevlrvif ptgdskvvlp cnllrmiwna qkifhinprl psdlhpikvv egvkelskkl
1021 vivngddpls rqaqenatll fnihlrstlc srrmaeefrl sgeafdwllg eieskfnqai
1081 ahpgemvgal aaqslgepat qmtlntfhya gvsaknvtlg vprlkelini skkpktpslt
1141 vfllgqsard aerakdilcr lehttlrkvt antaiyydpn pqstvvaedq ewvnvyyemp
1201 dfdvarispw llrveldrkh mtdrkltmeq iaekinagfg ddlncifndd naeklvlrir
1261 imnsdenkmq eeeevvdkmd ddvflrcies nmltdmtlqg ieqiskvymh lpqtdnkkki
1321 iitedgefka lqewiletdg vslmrvlsek dvdpvrttsn diveiftvlg ieavrkaler
1381 elyhvisfdg syvnyrhlal lcdtmtcrgh lmaitrhgvn rqdtgplmkc sfeetvdvlm
1441 eaaahgesdp mkgvseniml gqlapagtgc fdllldaekc kygmeiptni pglgaagptg
1501 mffgsapspm ggispamtpw nqgatpayga wspsvgsgmt pgaagfspsa asdasgfspg
1561 yspawsptpg spgspgpssp yipspggams psysptspay eprspggytp qspsysptsp
1621 sysptspsys ptspnyspts psysptspsy sptspsyspt spsysptsps ysptspsysp
1681 tspsysptsp sysptspsys ptspsyspts psysptspsy sptspsyspt spsysptsps
1741 ysptspnysp tspnytptsp sysptspsys ptspnytpts pnysptspsy sptspsyspt
1801 spsyspsspr ytpqsptytp sspsyspssp sysptspkyt ptspsyspss peytptspky
1861 sptspkyspt spkysptspt yspttpkysp tsptysptsp vytptspkys ptsptyspts
1921 pkysptspty sptspkgsty sptspgyspt sptysltspa ispddsdeen
//