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Binding affinity to EEF2 in human NCI-H338 cell lysate assessed as trypsin-mediated biotinylated cysteine residues digestion by measuring reduction in protein level at 4 uM pretreated for 2 hrs followed by labelled with iodoacetamide-desthiobiotin by MS analysis relative to control
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to elongation factor 2 in human DOHH-2 cells by MS analysis
Binding affinity to elongation factor 2 in human MDA-MB-453 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to elongation factor 2 in human DOHH-2 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Binding affinity to elongation factor 2 in human DOHH-2 cells measured after 6 hrs by pull-down assay
Binding affinity to elongation factor 2 in human MDA-MB-453 cells measured after 6 hrs by pull-down assay
Effect on EF2 phosphorylation in human MDA-MB-231 cells at 10 uM measured up to 24 hrs by Western blot analysis
Effect on EF2 phosphorylation in human MCF7 cells at 10 uM measured up to 24 hrs by Western blot analysis
Downregulation of EF2 phosphorylation in human MDA-MB-231 cells at 3 uM measured up to 36 hrs by Western blot analysis
Assay data:1 Active, 2 Tested
Downregulation of EF2 phosphorylation in human MCF7 cells at 1.5 uM measured up to 36 hrs by Western blot analysis
Binding affinity to eukaryotic translation initiation factor in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Binding affinity to elongation factor 2 in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Experimentally measured binding affinity data (Kd) for protein-ligand complexes derived from PDB
Assay data:3916 Active, 206 Activity ≤ 1 nM, 1830 Activity ≤ 1 µM, 3916 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of EFK2 assessed as residual enzyme activity at 10 uM
Inhibition of eEF2-mediated tRNA translocation assessed as induction of dipeptide accumulation by TLC
Assay data:2 Active, 2 Tested
Inhibition of eEF2-mediated tRNA translocation assessed as decrease in tripeptide (Met-Phe-Phe) formation by TLC
Inhibition of eEF2-mediated tRNA translocation assessed as inhibition of phenylalanyl puromycin formation
Inhibition of eEF2-mediated tRNA translocation assessed as inhibition of polyphenylalanine synthesis at 200 uM after 10 mins by scintillation counting
Experimentally measured binding affinity data derived from PDB
Assay data:3073 Active, 3073 Tested
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