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Inhibition of S-COMT (unknown origin)
Assay data:2 Tested
SummaryRelated BioAssays by Target
Inhibition of S-COMT (unknown origin) assessed as scopoletin formation by measuring fluorescence using 6,7-dihydroxycoumarin as substrate at 100 uM and measured for 60 mins by fluorescence based microplate reader assay
Assay data:6 Active, 11 Tested
Inhibition of S-COMT (unknown origin) assessed as scopoletin formation by measuring fluorescence using 6,7-dihydroxycoumarin as substrate at 10 uM and measured for 60 mins by fluorescence based microplate reader assay
Assay data:1 Active, 11 Tested
Inhibition of rat brain COMT using pyrocatechol as substrate by radiometric assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Induction of GNMT in human H7GPL cells assessed as fold increase in promoter-driven luciferase reporter expression at 2.5 uM measured after 24 hrs by luminescence assay relative to control
Assay data:26 Tested
Induction of GNMT in human H7GPL cells assessed as fold increase in promoter-driven luciferase reporter expression measured after 24 hrs by luminescence assay relative to control
Assay data:1 Tested
Induction of GNMT in human H7GPL cells assessed as fold increase in promoter-driven luciferase reporter expression at 10 uM measured after 24 hrs by luminescence assay relative to control
Assay data:29 Tested
Inhibition of full-length human wild-type NNMT assessed as formation of MNA at 0.5 uM using nicotinamide as substrate and SAM as cofactor preincubated for 10 mins followed by substrate addition and measured after 40 mins by by HILIC-MS analysis
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of NNMT (unknown origin) assessed as formation of MNA at 0.5 uM using nicotinamide as substrate and SAM as cofactor by fluorescence based assay
Inhibition of NNMT (unknown origin) assessed as formation of MNA using nicotinamide as substrate and SAM as cofactor by fluorescence based assay
Assay data:1 Active, 1 Tested
Inhibition of recombinant human NNMT assessed as formation of MNA using nicotinamide and AdoMet as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by UHP-HILIC-MS analysis
Inhibition of recombinant human NNMT expressed in Escherichia coli BL21 (DE3) assessed as Kobs/[I] ratio and measured after 20 mins using nicotinamide as substrate and SAM as cofactor by MTase-Glo reagent based assay
Inhibition of endogenous mouse NNMT overexpressed in murine 3T3-L1 cells assessed incubated for 24 hrs by LC-MS/MS analysis
Inhibition of endogenous human NNMT overexpressed in human U2OS cells assessed incubated for 24 hrs by LC-MS/MS analysis
Inhibition of human NNMT at 30 uM preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of mouse NNMT preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of human NNMT preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of recombinant human NNMT (1 to 270 residues) expressed in Escherichia coli BL21 (DE3) and measured upto 60 mins by fluorescence polarization assay
Binding affinity to recombinant human NNMT expressed in Escherichia coli BL21 (DE3) assessed as Km preincubated for 10 mins followed by NAM addition and measured after 10 mins by Michaelis-Menten analysis
Binding affinity to recombinant human NNMT SAM binding site expressed in Escherichia coli BL21 (DE3) using nicotinamide as substrate and AdoMet as cofactor preincubated for 10 mins followed by substrate addition and measured after 10 mins by UHP-HILIC-MS analysis
Assay data:1 Active, 2 Tested
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