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Inhibition of NPP3 (unknown origin) using p-Nph-5'-TMP as substrate
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human NPP3 expressed in COS-7 cells using p-Nph-5'-TMP as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by malachite green reagent based spectrophotometric method
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human NPP3 expressed in COS-7 cells using p-Nph-5'-TMP as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by spectrophotometric method
Inhibition of human NPP3 expressed in COS-7 cells using p-Nph-5'-TMP as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins
Inhibition of recombinant human NPP3 expressed in CHO cells using ATP as substrate measured after 20 mins by mini-capillary electrophoresis
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of recombinant human NPP3 expressed in HEK293 cells using p-Nph-5'-TMP as substrate measured after 60 mins by mini-capillary electrophoresis
Ratio of Ki for recombinant human soluble NPP3 expressed in Sf9 insect cells using ATP as substrate to Ki for recombinant human soluble NPP3 expressed in Sf9 insect cells using p-Nph-5'-TMP as substrate
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant human soluble NPP3 expressed in Sf9 insect cells using ATP as substrate measured after 4 hrs by capillary electrophoresis
Assay data:10 Active, 2 Activity ≤ 1 µM, 36 Tested
Inhibition of recombinant human soluble NPP3 expressed in Sf9 insect cells using p-Nph-5'-TMP as substrate measured after 30 mins
Inhibition of recombinant human soluble NPP3 expressed in Sf9 insect cells at 10 uM using p-Nph-5'-TMP as substrate measured after 30 mins relative to control
Competitive inhibition of human NPP3 transfected in COS7 cells at 0.1 to 1 uM using pNP-TMP as substrate incubated for 35 mins by Lineweaver-Burk plot analysis
Inhibition of human NPP3 transfected in COS7 cells at 100 uM using pNP-TMP as substrate incubated for 35 mins relative to control
Assay data:3 Tested
Inhibition of human NPP3 transfected in COS7 cells using pNP-TMP as substrate incubated for 35 mins
Assay data:9 Active, 4 Activity ≤ 1 µM, 11 Tested
Inhibition of NPP3 (unknown origin) transfected in COS7 cells using pNP-TMP as substrate incubated for 35 mins by spectrophotometric method
Potency index, ratio of IC50 for suramin to IC50 for test compound for human recombinant NPP3 expressed in COS-7 cell membrane using p-nitrophenyl thymidine monophosphate substrate incubated for 35 mins by spectrophotometric analysis
Inhibition of human recombinant NPP3 expressed in COS-7 cell membrane at 100 uM using p-nitrophenyl thymidine monophosphate substrate incubated for 35 mins by spectrophotometric analysis
Assay data:9 Active, 3 Activity ≤ 1 µM, 10 Tested
Inhibition of human recombinant NPP3 at 10 uM using p-nitrophenyl thymidine monophosphate substrate incubated for 30 mins by colorimetry
Assay data:4 Tested
Potency index, ratio of IC50 for suramin to IC50 for test compound for inhibition of human NPP3 expressed in African green monkey COS7 cell membranes pre-incubated for 10 mins before pNP-TMP substrate addition and further incubated for 15 mins by colorimetric method
Inhibition of human NPP3 expressed in African green monkey COS7 cell membranes pre-incubated for 10 mins before pNP-TMP substrate addition and further incubated for 15 mins by colorimetric method relative to untreated control
Assay data:10 Tested
Inhibition of human NPP3 expressed in African green monkey COS7 cell membranes pre-incubated for 10 mins before pNP-TMP substrate addition and further incubated for 15 mins by colorimetric method
Assay data:25 Active, 6 Activity ≤ 1 µM, 28 Tested
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