Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
SPR assay from Article 10.1021/jm101625x: "Adenosine-derived inhibitors of 78 kDa glucose regulated protein (Grp78) ATPase: insights into isoform selectivity."
Assay data:13 Active, 8 Activity ≤ 1 µM, 14 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Binding affinity to 78 kDa glucose-regulated protein in human A549 cells by LC-MS/MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to N-terminal His-tagged GRP78 (unknown origin) assessed as change in melting temperature at 500 uM by differential scanning fluorimetry
Assay data:5 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to human GRP78 at 1 uM measured after 30 mins by UF-ASMS analysis relative to control
Assay data:14 Tested
Inhibition of recombinant GRP78 substrate binding domain ( 419 to 633 residues) (unknown origin) expressed in Escherichia coli BL21 DE3 cells pre-incubated for 10 mins before FITC-NRLLLTG fluorescent peptide addition in presence of 20 uM ADP followed by further incubation for 2 hrs by fluorescence polarization assay
Inhibition of GRP78 in human HCT116 cells assessed as increase in unfolded protein response marker CHOP level measured after 16 hrs by Western blot analysis
Assay data:1 Tested
Inhibition of GRP78 in human HCT116 cells assessed as increase in unfolded protein response marker ATF4 level measured after 16 hrs by Western blot analysis
Inhibition of GRP78 in human HCT116 cells assessed as increase in unfolded protein response marker XBP1 level measured after 16 hrs by Western blot analysis
Inhibition of GRP78 (26 to 636 residues) (unknown origin) expressed in Escherichia coli BL21 DE3 cells pre-incubated for 10 mins before FITC-NRLLLTG fluorescent peptide addition in presence of 100 uM ADP followed by further incubation for 2 hrs by fluorescence polarization assay
Assay data:1 Active, 2 Tested
Inhibition of GRP78 (26 to 636 residues) (unknown origin) expressed in Escherichia coli BL21 DE3 cells pre-incubated for 10 mins before FITC-NRLLLTG fluorescent peptide addition in presence of 10 uM ADP followed by further incubation for 2 hrs by fluorescence polarization assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of GRP78 (26 to 636 residues) (unknown origin) expressed in Escherichia coli BL21 DE3 cells pre-incubated for 10 mins before FITC-NRLLLTG fluorescent peptide addition in presence of 20 uM ADP followed by further incubation for 2 hrs by fluorescence polarization assay
Assay data:4 Active, 10 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to 78 kDa glucose-regulated protein in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Induction of glucose-regulated protein 78 in HEK293 cells at 200 uM after 6 hrs by Western blotting method
Assay data:2 Active, 2 Tested
Inhibition of 2-deoxyglucose-induced GRP78 (unknown origin) expression transfected in human HT1080 cells by luciferase reporter gene assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Secondary Screen
Assay data:340 Active, 500 Tested
SummaryRelated BioAssays by Depositor
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on