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Binding affinity to EEF2 in human NCI-H338 cell lysate assessed as trypsin-mediated biotinylated cysteine residues digestion by measuring reduction in protein level at 4 uM pretreated for 2 hrs followed by labelled with iodoacetamide-desthiobiotin by MS analysis relative to control
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to elongation factor 2 in human DOHH-2 cells by MS analysis
Binding affinity to elongation factor 2 in human MDA-MB-453 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to elongation factor 2 in human DOHH-2 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Binding affinity to elongation factor 2 in human DOHH-2 cells measured after 6 hrs by pull-down assay
Binding affinity to elongation factor 2 in human MDA-MB-453 cells measured after 6 hrs by pull-down assay
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Effect on EF2 phosphorylation in human MDA-MB-231 cells at 10 uM measured up to 24 hrs by Western blot analysis
Effect on EF2 phosphorylation in human MCF7 cells at 10 uM measured up to 24 hrs by Western blot analysis
Downregulation of EF2 phosphorylation in human MDA-MB-231 cells at 3 uM measured up to 36 hrs by Western blot analysis
Assay data:1 Active, 2 Tested
Downregulation of EF2 phosphorylation in human MCF7 cells at 1.5 uM measured up to 36 hrs by Western blot analysis
Binding affinity to eukaryotic translation initiation factor in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Binding affinity to elongation factor 2 in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
Assay data:555 Active, 64752 Tested
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
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