Alternative titles; symbols
HGNC Approved Gene Symbol: JPT1
Cytogenetic location: 17q25.1 Genomic coordinates (GRCh38): 17:75,135,243-75,154,512 (from NCBI)
JPT1 is a microtubule-binding protein (Karpova et al., 2006).
Tang et al. (1997) cloned mouse Jpt1, which they called Hn1. The deduced protein contains 154 amino acids. Northern blot analysis detected Hn1 expression in all mouse tissues examined, with highest expression in hemopoietic and brain tissues.
Zhou et al. (2004) cloned human HN1 from a testis and brain cDNA library. The deduced 154-amino acid human protein shares 92% amino acid identity with mouse Hn1. Northern blot analysis detected HN1 transcripts of 1.0 and 1.6 kb in a wide range of human tissues, with abundant expression in testis, low expression in skeletal muscle, thymus, prostate, colon, and peripheral blood cells, and slight expression in brain and placenta. Western blot analysis showed that HN1 had an apparent molecular mass of about 16.5 kD in transfected human cells. Fluorescence-tagged HN1 localized to nuclei of transfected HeLa cells. HN1 is conserved from mammals to amphibians, with HN1 from human, chimpanzee, and macaca sharing the same protein sequence.
Karpova et al. (2006) identified the Drosophila ortholog of HN1, which they termed Jupiter. Jupiter is a soluble, unfolded molecule, rich in glycine, and with 2 repeats around the sequence PPGG separated by a serine-rich region. Confocal microscopic analysis showed that fluorescence-tagged Jupiter localized to the microtubule network throughout the cell cycle at different stages of Drosophila development, with accumulation in brain and adult ovary.
Zhou et al. (2004) determined that the JPT1 gene contains 5 exons and spans 19.4 kb.
By database analysis, Zhou et al. (2004) mapped the JPT1 gene to chromosome 17q25.2. A pseudogene, JPT1P1, is located on chromosome 2.
Tang et al. (1997) mapped the mouse Jpt1 gene to chromosome 11.
Using an in vitro binding assay, Karpova et al. (2006) showed that Drosophila Jupiter bound microtubules. Binding affinity was low for microtubules but high for taxol-stabilized microtubules. Distribution of fluorescence-tagged Jupiter reproduced microtubule behavior in transfected Drosophila cells.
Karpova, N., Bobinnec, Y., Fouix, S., Huitorel, P., Debec, A. Jupiter, a new Drosophila protein associated with microtubules. Cell Motil. Cytoskeleton 63: 301-312, 2006. [PubMed: 16518797] [Full Text: https://doi.org/10.1002/cm.20124]
Tang, W., Lai, Y.-H., Han, X.-D., Wong, P. M. C., Peters, L. L., Cui, D. H. K. Murine Hn1 on chromosome 11 is expressed in hematopoietic and brain tissues. Mammalian Genome 8: 695-696, 1997. [PubMed: 9271675] [Full Text: https://doi.org/10.1007/s003359900540]
Zhou, G., Wang, J., Zhang, Y., Zhong, C., Ni, J., Wang, L., Guo, J., Zhang, K., Yu, L., Zhao, S. Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family. Gene 331: 115-123, 2004. [PubMed: 15094197] [Full Text: https://doi.org/10.1016/j.gene.2004.02.025]