Prg4 proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prg4provided by MGI
Official Full Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)provided by MGI
Primary source: MGI:MGI:1891344
See related: Ensembl:ENSMUSG00000006014 AllianceGenome:MGI:1891344
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MSF; SZP; CACP; JCAP; DOL54; lubricin
Summary: Predicted to enable polysaccharide binding activity and scavenger receptor activity. Acts upstream of or within hematopoietic stem cell proliferation; negative regulation of interleukin-6 production; and regulation of cell population proliferation. Located in extracellular space. Is expressed in brain; larynx; musculoskeletal system; and spinal component of peripheral nervous system. Human ortholog(s) of this gene implicated in camptodactyly-arthropathy-coxa vara-pericarditis syndrome and pericarditis. Orthologous to human PRG4 (proteoglycan 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver adult (RPKM 3.0), heart adult (RPKM 1.6) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 G1; 1 63.84 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (150325163..150341916, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (150449412..150466221, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Proteoglycan 4 is present within the dura mater and produced by mesenchymal progenitor cells.
Title: Proteoglycan 4 is present within the dura mater and produced by mesenchymal progenitor cells.
Regulatory Mechanisms of Prg4 and Gdf5 Expression in Articular Cartilage and Functions in Osteoarthritis.
Title: Regulatory Mechanisms of Prg4 and Gdf5 Expression in Articular Cartilage and Functions in Osteoarthritis.
Systematic expression analysis of plasticity-related genes in mouse brain development brings PRG4 into play.
Title: Systematic expression analysis of plasticity-related genes in mouse brain development brings PRG4 into play.
Proteoglycan-4 is an essential regulator of synovial macrophage polarization and inflammatory macrophage joint infiltration.
Title: Proteoglycan-4 is an essential regulator of synovial macrophage polarization and inflammatory macrophage joint infiltration.
Wnt/beta-catenin signaling contributes to articular cartilage homeostasis through lubricin induction in the superficial zone.
Title: Wnt/β-catenin signaling contributes to articular cartilage homeostasis through lubricin induction in the superficial zone.
PRG4 plays a role in the maturation of the subchondral bone
Title: Absence of Proteoglycan 4 (Prg4) Leads to Increased Subchondral Bone Porosity Which Can Be Mitigated Through Intra-Articular Injection of PRG4.
Combinatorial Prg4 and Il-1ra Gene Therapy Protects Against Hyperalgesia and Cartilage Degeneration in Post-Traumatic Osteoarthritis.
Title: Combinatorial Prg4 and Il-1ra Gene Therapy Protects Against Hyperalgesia and Cartilage Degeneration in Post-Traumatic Osteoarthritis.
Prg4 is present in macrophages in atherosclerotic lesions and critically influences macrophage function, but deletion of Prg4 in bone marrow-derived cells does not affect atherosclerotic lesion development.
Title: Proteoglycan 4 regulates macrophage function without altering atherosclerotic lesion formation in a murine bone marrow-specific deletion model.
Prg4 deficiency increases atherosclerosis susceptibility in apolipoprotein E knockout and low-density lipoprotein receptor knockout mice
Title: Total body proteoglycan 4 (Prg4) deficiency increases atherosclerosis susceptibility in apolipoprotein E knockout and low-density lipoprotein receptor knockout mice.
These results indicate that maintenance of Prg4 expression prevents osteoarthritis progression associated with reduced TGF-beta signaling in mice.
Title: Prg4 prevents osteoarthritis induced by dominant-negative interference of TGF-ß signaling in mice.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables polysaccharide binding	IEA Inferred from Electronic Annotation more info
enables scavenger receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within hematopoietic stem cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of interleukin-6 production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of interleukin-6 production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	HDA more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteoglycan 4

Names: megakaryocyte-stimulating factor; proteoglycan 3; superficial zone proteoglycan

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110146.1 → NP_001103616.1 proteoglycan 4 isoform 2 precursor

See identical proteins and their annotated locations for NP_001103616.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks internal coding exons and segments, as compared to variant 1. The resulting isoform (2) is much shorter but has identical N- and C-termini, compared to isoform 1.

Source sequence(s)

AK149469, BE457731

Consensus CDS

CCDS48391.1

UniProtKB/TrEMBL

A0A0R4J207, A1L3C5

Related

ENSMUSP00000125551.2, ENSMUST00000162367.8

Conserved Domains (2) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

cd00094
Location:161 → 422

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
NM_021400.3 → NP_067375.3 proteoglycan 4 isoform 1 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC161432

Consensus CDS

CCDS48392.1

UniProtKB/TrEMBL

E0CZ58, E9QQ17

Related

ENSMUSP00000125677.2, ENSMUST00000161611.8

Conserved Domains (4) summary

PTZ00449
Location:634 → 938

PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:446 → 761

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:959 → 1220

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

150325163..150341916 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154551.1 → XP_036010444.1 proteoglycan 4 isoform X11

UniProtKB/TrEMBL

E9QQ18

Conserved Domains (4) summary

PTZ00449
Location:505 → 809

PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional

smart00201
Location:25 → 65

SO; Somatomedin B -like domains

PHA03247
Location:317 → 632

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:830 → 1091

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154540.1 → XP_036010433.1 proteoglycan 4 isoform X4

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

PTZ00449
Location:593 → 897

PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional

smart00201
Location:25 → 67

SO; Somatomedin B -like domains

PHA03247
Location:405 → 720

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:918 → 1179

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154548.1 → XP_036010441.1 proteoglycan 4 isoform X8

UniProtKB/TrEMBL

E9QQ18

Conserved Domains (5) summary

PTZ00449
Location:546 → 850

PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:358 → 673

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:871 → 1132

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

pfam01033
Location:69 → 106

Somatomedin_B; Somatomedin B domain
XM_036154543.1 → XP_036010436.1 proteoglycan 4 isoform X5

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:402 → 722

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:912 → 1173

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

NF033201
Location:667 → 784

Vip_LPXTG_Lm; cell invasion LPXTG protein Vip
XM_036154555.1 → XP_036010448.1 proteoglycan 4 isoform X13

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (2) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

cd00094
Location:672 → 933

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154534.1 → XP_036010427.1 proteoglycan 4 isoform X2

UniProtKB/Swiss-Prot

Q3UEL1, Q3V198, Q9JM99

Conserved Domains (3) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:428 → 795

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:935 → 1196

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154553.1 → XP_036010446.1 proteoglycan 4 isoform X12

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:452 → 819

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:823 → 1084

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

pfam02389
Location:517 → 633

Cornifin; Cornifin (SPRR) family
XM_036154547.1 → XP_036010440.1 proteoglycan 4 isoform X7

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:452 → 875

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:879 → 1140

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

pfam02389
Location:573 → 689

Cornifin; Cornifin (SPRR) family
XM_036154549.1 → XP_036010442.1 proteoglycan 4 isoform X9

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:403 → 859

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:863 → 1124

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

pfam02389
Location:557 → 673

Cornifin; Cornifin (SPRR) family
XM_036154550.1 → XP_036010443.1 proteoglycan 4 isoform X10

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (4) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:452 → 724

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:855 → 1116

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...

pfam02389
Location:501 → 617

Cornifin; Cornifin (SPRR) family
XM_036154546.1 → XP_036010439.1 proteoglycan 4 isoform X6

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (3) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:452 → 748

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:879 → 1140

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154538.1 → XP_036010431.1 proteoglycan 4 isoform X3

UniProtKB/TrEMBL

E9QQ17

Conserved Domains (3) summary

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:403 → 779

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:919 → 1180

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...
XM_036154525.1 → XP_036010418.1 proteoglycan 4 isoform X1

UniProtKB/TrEMBL

E0CZ58, E9QQ17

Related

ENSMUSP00000128943.2, ENSMUST00000164600.8

Conserved Domains (4) summary

PTZ00449
Location:634 → 938

PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional

smart00201
Location:26 → 68

SO; Somatomedin B -like domains

PHA03247
Location:446 → 761

PHA03247; large tegument protein UL36; Provisional

cd00094
Location:959 → 1220

HX; Hemopexin-like repeats.; Hemopexin is a heme-binding protein that transports heme to the liver. Hemopexin-like repeats occur in vitronectin and some matrix metalloproteinases family (matrixins). The HX repeats of some matrixins bind tissue inhibitor of ...