Dnaja3 DnaJ heat shock protein family (Hsp40) member A3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dnaja3provided by MGI
Official Full Name: DnaJ heat shock protein family (Hsp40) member A3provided by MGI
Primary source: MGI:MGI:1933786
See related: Ensembl:ENSMUSG00000004069 AllianceGenome:MGI:1933786
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tid-1; Tid1l; 1200003J13Rik; 1810053A11Rik
Summary: Enables GTPase regulator activity; receptor tyrosine kinase binding activity; and unfolded protein binding activity. Involved in skeletal muscle acetylcholine-gated channel clustering. Acts upstream of or within several processes, including activation-induced cell death of T cells; mitochondrial DNA replication; and positive regulation of T cell proliferation. Located in several cellular components, including mitochondrion; neuromuscular junction; and postsynaptic membrane. Is extrinsic component of plasma membrane. Orthologous to human DNAJA3 (DnaJ heat shock protein family (Hsp40) member A3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in heart adult (RPKM 29.3), kidney adult (RPKM 24.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 16 A1; 16 2.46 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (4501934..4525559)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (4684070..4707695)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Targeting the MYC interaction network in B-cell lymphoma via histone deacetylase 6 inhibition.
Title: Targeting the MYC interaction network in B-cell lymphoma via histone deacetylase 6 inhibition.
Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.
Title: Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.
Tid1 deficiency reduces ATP production.
Title: Mitochondrial co-chaperone protein Tid1 is required for energy homeostasis during skeletal myogenesis.
Dnaja3 is crucial for mitochondrial biogenesis through its chaperone activity on Polga and has a role in preventing dilated cardiomyopathy
Title: A crucial role of mitochondrial Hsp40 in preventing dilated cardiomyopathy.
Overexpression of the N-terminal half of Tid1 induced agrin- and MuSK-independent phosphorylation and clustering of AChRs. These results demonstrate that Tid1 is an essential component of the agrin signaling pathway, crucial for synaptic development.
Title: A mammalian homolog of Drosophila tumorous imaginal discs, Tid1, mediates agrin signaling at the neuromuscular junction.
It is proposed that Tid1 contributes to cell aging by acting as a repressor of NF-kappa B signaling.
Title: Functional genetic screen for genes involved in senescence: role of Tid1, a homologue of the Drosophila tumor suppressor l(2)tid, in senescence and cell survival.
Tid1 is critical for early mammalian development, most likely for its function in sustaining embryonic-cell survival, which requires its association with Hsp70.
Title: Tid1, a cochaperone of the heat shock 70 protein and the mammalian counterpart of the Drosophila tumor suppressor l(2)tid, is critical for early embryonic development and cell survival.
Tid-1S in Th2 cells following activation has a role in induction of apoptosis resistance during the activation of Th2 cells
Title: TID1, a mammalian homologue of the drosophila tumor suppressor lethal(2) tumorous imaginal discs, regulates activation-induced cell death in Th2 cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C81173 (e-PCR)
- UniSTS:209154

RH127020 (e-PCR)
- UniSTS:209158

NoName (e-PCR)
- UniSTS:237416

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables GTPase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables Hsp70 protein binding	ISO Inferred from Sequence Orthology more info
enables IkappaB kinase complex binding	ISO Inferred from Sequence Orthology more info
enables NF-kappaB binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables heat shock protein binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables receptor tyrosine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables type II interferon receptor binding	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within T cell differentiation in thymus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in activation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within activation-induced cell death of T cells	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular senescence	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within embryo development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitochondrial DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of type II interferon-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in neuromuscular junction development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuromuscular junction development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein folding	ISO Inferred from Sequence Orthology more info
involved_in protein stabilization	ISO Inferred from Sequence Orthology more info
involved_in response to heat	IEA Inferred from Electronic Annotation more info
involved_in response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in skeletal muscle acetylcholine-gated channel clustering	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within small GTPase-mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with actin filament	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
located_in mitochondrial nucleoid	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: dnaJ homolog subfamily A member 3, mitochondrial

Names: DnaJ (Hsp40) homolog, subfamily A, member 3; dnaJ protein Tid-1; mTid-1; tumorous imaginal discs protein Tid56 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001135112.1 → NP_001128584.1 dnaJ homolog subfamily A member 3, mitochondrial isoform 2

See identical proteins and their annotated locations for NP_001128584.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an exon in the 3' region, as compared to variant 1. The resulting isoform (2) has a shorter and different C-terminus, as compared to isoform 1.

Source sequence(s)

AK167511, BP757709, CJ148764

Consensus CDS

CCDS49748.1

UniProtKB/TrEMBL

Q3TJA9

Related

ENSMUSP00000111520.4, ENSMUST00000115854.4

Conserved Domains (4) summary

cd10719
Location:236 → 296

DnaJ_zf; Zinc finger domain of DnaJ and HSP40

cd10747
Location:207 → 416

DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40

COG0484
Location:90 → 435

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:93 → 155

DnaJ; DnaJ domain
NM_023646.4 → NP_076135.3 dnaJ homolog subfamily A member 3, mitochondrial isoform 1

See identical proteins and their annotated locations for NP_076135.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK004910, AK011535, BP757709, CJ148764

Consensus CDS

CCDS27922.1

UniProtKB/Swiss-Prot

Q8BSM0, Q99L09, Q99M87, Q99P71, Q99P76, Q9CT11, Q9DBJ7, Q9DC44

Related

ENSMUSP00000053842.6, ENSMUST00000060067.12

Conserved Domains (1) summary

COG0484
Location:90 → 480

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]