Spaca3 sperm acrosome associated 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spaca3provided by MGI
Official Full Name: sperm acrosome associated 3provided by MGI
Primary source: MGI:MGI:1922872
See related: Ensembl:ENSMUSG00000053184 AllianceGenome:MGI:1922872
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Lyc3; SLLP1; ALLP17; mSLLP1; 1700025M08Rik
Summary: Predicted to enable lysozyme activity. Involved in fusion of sperm to egg plasma membrane involved in single fertilization. Acts upstream of or within sperm-egg recognition. Located in acrosomal vesicle. Orthologous to human SPACA3 (sperm acrosome associated 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward testis adult (RPKM 156.7) See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 48.4 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (80749189..80758640)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (80858363..80867814)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

distinct from c-lysozyme by substituting two essential catalytic residues (E35T/D52N), and by forming helical filaments approximately 75 A in diameter with a 25 A central pore comprised of six monomers per helix turn repeating every 33 A
Title: Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.
Female mice immunized with SPRASA had profound infertility following timed matings and those mice that did become pregnant had reduced fetal viability.
Title: The role of SPRASA in female fertility.
Data show that SAS1B/SAS1R (Sperm Acrosomal SLLP1 Binding/Sperm Acrosomal SLLP1 receptor) bound to sperm acrosomal protein SlLP1 with high affinity.
Title: Oocyte specific oolemmal SAS1B involved in sperm binding through intra-acrosomal SLLP1 during fertilization.
This study demonstrates the retention of mouse sperm lysozyme-like protein (mSLLP1) in the equatorial segment of spermatozoa following the acrosome reaction and a role for mSLLP1 in sperm-egg binding and fertilization.
Title: Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

NoName (e-PCR)
- UniSTS:487980

NoName (e-PCR)
- UniSTS:488927

D11Bhm128 (e-PCR), detects polymorphism
- UniSTS:141565

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables lysozyme activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IBA Inferred from Biological aspect of Ancestor more info
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sperm-egg recognition	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in acrosomal matrix	ISO Inferred from Sequence Orthology more info
located_in acrosomal membrane	ISO Inferred from Sequence Orthology more info
is_active_in acrosomal vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in acrosomal vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info
is_active_in sperm flagellum	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: sperm acrosome membrane-associated protein 3

Names: acrosomal sperm protein; lysozyme-like protein 3; sperm c-type lysozyme-like protein; sperm lysozyme-like protein 1 precusor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359183.1 → NP_001346112.1 sperm acrosome membrane-associated protein 3 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AL645842

Consensus CDS

CCDS25136.1

UniProtKB/TrEMBL

A0A077S9M6

Related

ENSMUSP00000099512.2, ENSMUST00000103223.8

Conserved Domains (1) summary

cd00119
Location:36 → 159

LYZ1; C-type lysozyme (1, 4-beta-N-acetylmuramidase, LYZ) and alpha-lactalbumin (lactose synthase B protein, LA). They have a close evolutionary relationship and similar tertiary structure, however, functionally they are quite different. Lysozymes have ...
NM_029367.1 → NP_083643.1 sperm acrosome membrane-associated protein 3 precursor

See identical proteins and their annotated locations for NP_083643.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

BC100503

Consensus CDS

CCDS25136.1

UniProtKB/TrEMBL

A0A077S9M6

Related

ENSMUSP00000099511.4, ENSMUST00000103222.4

Conserved Domains (1) summary

cd00119
Location:36 → 159

LYZ1; C-type lysozyme (1, 4-beta-N-acetylmuramidase, LYZ) and alpha-lactalbumin (lactose synthase B protein, LA). They have a close evolutionary relationship and similar tertiary structure, however, functionally they are quite different. Lysozymes have ...