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Spaca6 sperm acrosome associated 6 [ Mus musculus (house mouse) ]

Gene ID: 75202, updated on 5-Mar-2024

Summary

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Official Symbol
Spaca6provided by MGI
Official Full Name
sperm acrosome associated 6provided by MGI
Primary source
MGI:MGI:1922452
See related
Ensembl:ENSMUSG00000080316 AllianceGenome:MGI:1922452
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
let-7e; SPACA6A; mir-99b; mir-125a; Ncrna00085; 4930546H06Rik; 9530079D04Rik; B230206P06Rik
Summary
Involved in fusion of sperm to egg plasma membrane involved in single fertilization. Located in acrosomal vesicle. Orthologous to human SPACA6 (sperm acrosome associated 6). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in CNS E11.5 (RPKM 6.5), limb E14.5 (RPKM 5.9) and 24 other tissues See more
Orthologs
human all
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Genomic context

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Location:
17 A3.2; 17 10.5 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (18045955..18063274)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (17826085..17843010)

Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene farnesyl diphosphate synthetase pseudogene Neighboring gene vomeronasal 2, receptor 90 Neighboring gene STARR-seq mESC enhancer starr_41964 Neighboring gene STARR-seq mESC enhancer starr_41965 Neighboring gene STARR-seq mESC enhancer starr_41966 Neighboring gene STARR-seq mESC enhancer starr_41967 Neighboring gene STARR-seq mESC enhancer starr_41968 Neighboring gene microRNA let7e Neighboring gene microRNA 125a Neighboring gene microRNA 99b Neighboring gene predicted gene, 36907 Neighboring gene hyaluronan synthase 1 Neighboring gene formyl peptide receptor 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function. Bhatlekar S, et al. Blood Cells Mol Dis, 2021 Dec. PMID 34775219, Free PMC Article
  2. Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion. Barbaux S, et al. Sci Rep, 2020 Mar 24. PMID 32210282, Free PMC Article
  3. Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments. Grossman H, et al. Sci Rep, 2017 May 22. PMID 28533542, Free PMC Article
  4. miR-125a-5p impairs endothelial cell angiogenesis in aging mice via RTEF-1 downregulation. Che P, et al. Aging Cell, 2014 Oct. PMID 25059272, Free PMC Article
  5. MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells. Xu Y, et al. J Agric Food Chem, 2021 Mar 3. PMID 33599498

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function.
    Title: Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster lowers platelet count without altering platelet function.
  2. MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells.
    Title: MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells.
  3. Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.
    Title: Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.
  4. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
    Title: Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
  5. Our findings suggest a mechanism, by which a decrease in miR-125a-3p during oocyte maturation facilitates GVBD by allowing Fyn up-regulation and the resulting stabilization of the interaction between actin and A-type lamins.
    Title: Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase through modulation of actin filaments.
  6. Dual-luciferase reporter gene analysis showed that RTEF-1 is a direct target of mir-125a-5p, which regulates angiogenesis by repressing RTEF-1 expression and modulating eNOS and VEGF expression.
    Title: miR-125a-5p impairs endothelial cell angiogenesis in aging mice via RTEF-1 downregulation.
  7. The Spaca6 gene product may, together with Izumo1, mediate sperm fusion by binding an as yet unidentified egg membrane receptor.
    Title: A transgenic insertion on mouse chromosome 17 inactivates a novel immunoglobulin superfamily gene potentially involved in sperm-egg fusion.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within fusion of sperm to egg plasma membrane involved in single fertilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within single fertilization IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in acrosomal vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
sperm acrosome membrane-associated protein 6
Names
immunoglobulin domain-containing protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001162909.1 → NP_001156381.1  sperm acrosome membrane-associated protein 6 precursor

    Status: PROVISIONAL

    Source sequence(s)
    AC165361
    Consensus CDS
    CCDS49967.1
    UniProtKB/Swiss-Prot
    B7ZWC7, D3Z1S3, E9Q8Q8, J3QMI7
    Related
    ENSMUSP00000128732.3, ENSMUST00000172097.9
    Conserved Domains (2) summary
    smart00410
    Location:172 → 242
    IG_like; Immunoglobulin like
    cl11960
    Location:175 → 245
    Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000083.7 Reference GRCm39 C57BL/6J

    Range
    18045955..18063274
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011246692.4 → XP_011244994.1  sperm acrosome membrane-associated protein 6 isoform X7

  2. XM_017317705.3 → XP_017173194.1  sperm acrosome membrane-associated protein 6 isoform X9

    Conserved Domains (1) summary
    pfam12101
    Location:58 → 78
    DUF3577; Protein of unknown function (DUF3577)

  3. XM_006525053.4 → XP_006525116.1  sperm acrosome membrane-associated protein 6 isoform X1

  4. XM_036160818.1 → XP_036016711.1  sperm acrosome membrane-associated protein 6 isoform X2

  5. XM_006525055.5 → XP_006525118.1  sperm acrosome membrane-associated protein 6 isoform X3

    Conserved Domains (1) summary
    cl09930
    Location:12 → 70
    RPA_2b-aaRSs_OBF_like; Replication protein A, class 2b aminoacyl-tRNA synthetases, and related proteins with oligonucleotide/oligosaccharide (OB) fold.

  6. XM_036160819.1 → XP_036016712.1  sperm acrosome membrane-associated protein 6 isoform X5

  7. XM_006525056.5 → XP_006525119.1  sperm acrosome membrane-associated protein 6 isoform X4

  8. XM_030250121.2 → XP_030105981.1  sperm acrosome membrane-associated protein 6 isoform X6

  9. XM_011246695.4 → XP_011244997.1  sperm acrosome membrane-associated protein 6 isoform X8

    See identical proteins and their annotated locations for XP_011244997.1

    Conserved Domains (1) summary
    pfam12101
    Location:58 → 78
    DUF3577; Protein of unknown function (DUF3577)

RNA

  1. XR_385379.5 RNA Sequence

  2. XR_876567.4 RNA Sequence

  3. XR_876568.4 RNA Sequence

  4. XR_876572.4 RNA Sequence

  5. XR_003952208.2 RNA Sequence

  6. XR_385377.4 RNA Sequence

  7. XR_003952205.2 RNA Sequence

  8. XR_876569.4 RNA Sequence

  9. XR_385378.5 RNA Sequence

  10. XR_004939594.1 RNA Sequence

  11. XR_003952206.2 RNA Sequence

  12. XR_004939595.1 RNA Sequence

  13. XR_004939596.1 RNA Sequence

  14. XR_004939593.1 RNA Sequence

  15. XR_003952207.2 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_029205.2: Suppressed sequence

    Description
    NM_029205.2: This RefSeq was permanently suppressed because currently there is support for the transcript but not for the protein.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
E9Q8Q8.1 GenPept UniProtKB/Swiss-Prot:E9Q8Q8

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