Meiob meiosis specific with OB domains [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Meiobprovided by MGI
Official Full Name: meiosis specific with OB domainsprovided by MGI
Primary source: MGI:MGI:1922428
See related: Ensembl:ENSMUSG00000024155 AllianceGenome:MGI:1922428
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MLZ-675; 4930528F23Rik
Summary: Enables chromatin binding activity; single-stranded DNA 3'-5' exodeoxyribonuclease activity; and single-stranded DNA binding activity. Involved in double-strand break repair via homologous recombination; fertilization; and meiosis I. Acts upstream of or within male meiotic nuclear division. Located in cytoplasm. Is expressed in genitourinary system. Human ortholog(s) of this gene implicated in spermatogenic failure 22. Orthologous to human MEIOB (meiosis specific with OB-fold). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 1.7), liver E14 (RPKM 0.5) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.53 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (25023275..25058762)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (24804301..24839788)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The ssDNA-binding protein MEIOB acts as a dosage-sensitive regulator of meiotic recombination.
Title: The ssDNA-binding protein MEIOB acts as a dosage-sensitive regulator of meiotic recombination.
Two mutations (N64I and S67 deletion) in MEIOB cause self-aggregation of MEIOB and sharply reduced protein half-life. In addition to a role in early meiotic recombination, MEIOB/SPATA22 plays a role in late meiotic recombination, i.e., in crossover formation, in a dosage-dependent manner.
Title: The ssDNA-binding protein MEIOB acts as a dosage-sensitive regulator of meiotic recombination.
Aspartic acid 383 (D383) in MEIOB is essential for meiosis in mice. Isocotoin, a small molecule, inhibits the MEIOB-SPATA22 interaction and promotes their degradation in HEK293 cells in a dose-dependent manner.
Title: A cell-based high-content screen identifies isocotoin as a small molecule inhibitor of the meiosis-specific MEIOB-SPATA22 complex†.
We find that MEIOB and SPATA22 form an obligate complex and contain defined interaction domains
Title: Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein A complex and DNA double-strand breaks.
MEIOB and SPATA22 form an obligate complex and contain defined interaction domains. A single residue D383 in MEIOB is indispensable for the MEIOB-SPATA22 interaction. The MEIOB/SPATA22 complex interacts with the RPA heterotrimeric complex in a collaborative manner.
Title: Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein A complex and DNA double-strand breaks.
MEIOB is required for meiotic recombination and chromosomal synapsis.
Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.
MEIOB is a meiosis-specific paralogue of RPA1. MEIOB colocalizes with RPA in foci on meiotic chromosomes. MEIOB binds to single-stranded DNA and exhibits 3'-5' exonuclease activity. MEIOB forms a complex with RPA and with SPATA22. Chromatin localization and stability of MEIOB depends on SPATA22 and vice versa. MEIOB is essential for meiotic recombination in both sexes.
Title: MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (5) 1 citation

General gene information

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Markers

D6Got142 (e-PCR)
- UniSTS:115442

NoName (e-PCR)
- UniSTS:492789

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA 3'-5' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in homologous chromosome pairing at meiosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiotic nuclear division	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
involved_in resolution of meiotic recombination intermediates	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: meiosis-specific with OB domain-containing protein

Names: protein expressed in male leptotene and zygotene spermatocytes 675

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_029197.1 → NP_083473.1 meiosis-specific with OB domain-containing protein

See identical proteins and their annotated locations for NP_083473.1

Status: VALIDATED

Source sequence(s)

AC166102

Consensus CDS

CCDS50020.1

UniProtKB/Swiss-Prot

E9QLG1, Q9D513

UniProtKB/TrEMBL

A0A3Q4EGA1

Related

ENSMUSP00000024972.6, ENSMUST00000024972.7

Conserved Domains (2) summary

cd04475
Location:167 → 269

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:165 → 408

rpa1; replication factor-a protein 1 (rpa1)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

25023275..25058762

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006525045.4 → XP_006525108.1 meiosis-specific with OB domain-containing protein isoform X1

See identical proteins and their annotated locations for XP_006525108.1

UniProtKB/Swiss-Prot

E9QLG1, Q9D513

UniProtKB/TrEMBL

A0A3Q4EGA1

Conserved Domains (2) summary

cd04475
Location:167 → 269

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:165 → 408

rpa1; replication factor-a protein 1 (rpa1)
XM_017317702.2 → XP_017173191.1 meiosis-specific with OB domain-containing protein isoform X2

UniProtKB/TrEMBL

A0A3Q4EGA1

Conserved Domains (2) summary

cd04475
Location:167 → 269

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:165 → 405

rpa1; replication factor-a protein 1 (rpa1)
XM_006525047.4 → XP_006525110.1 meiosis-specific with OB domain-containing protein isoform X1

See identical proteins and their annotated locations for XP_006525110.1

UniProtKB/Swiss-Prot

E9QLG1, Q9D513

UniProtKB/TrEMBL

A0A3Q4EGA1

Conserved Domains (2) summary

cd04475
Location:167 → 269

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:165 → 408

rpa1; replication factor-a protein 1 (rpa1)
XM_006525048.5 → XP_006525111.1 meiosis-specific with OB domain-containing protein isoform X1

See identical proteins and their annotated locations for XP_006525111.1

UniProtKB/Swiss-Prot

E9QLG1, Q9D513

UniProtKB/TrEMBL

A0A3Q4EGA1

Conserved Domains (2) summary

cd04475
Location:167 → 269

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:165 → 408

rpa1; replication factor-a protein 1 (rpa1)
XM_006525052.4 → XP_006525115.1 meiosis-specific with OB domain-containing protein isoform X3

UniProtKB/TrEMBL

A0A3Q4EGA1

Conserved Domains (2) summary

cd04475
Location:45 → 147

RPA1_DBD_B; A subfamily of OB folds corresponding to the third OB fold, the ssDNA-binding domain (DBD)-B, of human RPA1 (also called RPA70). RPA1 is the large subunit of Replication protein A (RPA). RPA is a nuclear ssDNA-binding protein (SSB) which appears to be ...

TIGR00617
Location:43 → 286

rpa1; replication factor-a protein 1 (rpa1)