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Brf1 BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit [ Mus musculus (house mouse) ]

Gene ID: 72308, updated on 21-Apr-2024

Summary

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Official Symbol
Brf1provided by MGI
Official Full Name
BRF1, RNA polymerase III transcription initiation factor 90 kDa subunitprovided by MGI
Primary source
MGI:MGI:1919558
See related
Ensembl:ENSMUSG00000011158 AllianceGenome:MGI:1919558
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
GTF3B; TAF3C; TAFIII90; TFIIIB90; mTFIIIB90; 2510002F24Rik
Summary
Predicted to enable RNA polymerase III general transcription initiation factor activity and RNA polymerase III type 3 promoter sequence-specific DNA binding activity. Acts upstream of or within regulation of mRNA stability. Predicted to be part of transcription factor TFIIIB complex and transcription preinitiation complex. Predicted to be active in nucleus. Human ortholog(s) of this gene implicated in cerebellofaciodental syndrome. Orthologous to human BRF1 (BRF1 RNA polymerase III transcription initiation factor subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in thymus adult (RPKM 20.5), ovary adult (RPKM 14.2) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
12 F1; 12 61.47 cM
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (112919251..112964327, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (112955631..113000706, complement)

Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene jagged 2 Neighboring gene microRNA 6941 Neighboring gene STARR-positive B cell enhancer ABC_E11526 Neighboring gene STARR-seq mESC enhancer starr_33372 Neighboring gene nudix hydrolase 14 Neighboring gene BTB domain containing 6 Neighboring gene RIKEN cDNA 9230104M06 gene Neighboring gene STARR-positive B cell enhancer ABC_E1866 Neighboring gene phosphofurin acidic cluster sorting protein 2 Neighboring gene CapStarr-seq enhancer MGSCv37_chr12:114285178-114285287 Neighboring gene CapStarr-seq enhancer MGSCv37_chr12:114285299-114285486

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas. Liko D, et al. Cell Death Differ, 2019 Dec. PMID 30858608, Free PMC Article
  2. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. Zhong S, et al. J Biol Chem, 2011 Jan 28. PMID 21106530, Free PMC Article
  3. Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. Raineri I, et al. Nucleic Acids Res, 2004. PMID 14976220, Free PMC Article
  4. BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites. Benjamin D, et al. Mol Cell Biol, 2006 Dec. PMID 17030608, Free PMC Article
  5. Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase. Tan FE, et al. Proc Natl Acad Sci U S A, 2014 Apr 29. PMID 24733888, Free PMC Article

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Brf1 (zfp36l1), an AU-rich element mRNA-binding protein from the Zfp36 protein family, operates downstream of FGF/Erk MAP kinase signaling to regulate pluripotency and cell fate decision making in mouse embryonic stem cells.
    Title: Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.
  2. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression.
    Title: Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression.
  3. Phosphorylation at serine-92 and serine-203 is involved in BRF1 protein stability; high BRF1 protein levels are required to reinstate decay upon dissipation of the stabilizing signal.
    Title: BRF1 protein turnover and mRNA decay activity are regulated by protein kinase B at the same phosphorylation sites.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (4)  1 citation
  • Endonuclease-mediated (1) 

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RNA polymerase III general transcription initiation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase III type 3 promoter sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables TBP-class protein binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA-templated transcription initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of transcription by RNA polymerase III ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within regulation of mRNA stability IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transcription by RNA polymerase III IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in transcription preinitiation complex assembly IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription factor TFIIIB complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription preinitiation complex IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
transcription factor IIIB 90 kDa subunit
Names
B-related factor 1
BRF-1
BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB
hTFIIIB90

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_028193.3 → NP_082469.2  transcription factor IIIB 90 kDa subunit

    See identical proteins and their annotated locations for NP_082469.2

    Status: VALIDATED

    Source sequence(s)
    AC073562, AC160929
    Consensus CDS
    CCDS49195.1
    UniProtKB/Swiss-Prot
    Q8CFK2
    UniProtKB/TrEMBL
    G3X8S2
    Related
    ENSMUSP00000011302.8, ENSMUST00000011302.9
    Conserved Domains (2) summary
    COG1405
    Location:4 → 266
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:449 → 546
    BRF1; Brf1-like TBP-binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000078.7 Reference GRCm39 C57BL/6J

    Range
    112919251..112964327 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006516261.5 → XP_006516324.1  transcription factor IIIB 90 kDa subunit isoform X2

    UniProtKB/Swiss-Prot
    Q8CFK2
    Conserved Domains (2) summary
    COG1405
    Location:4 → 266
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:448 → 545
    BRF1; Brf1-like TBP-binding domain

  2. XM_006516260.5 → XP_006516323.1  transcription factor IIIB 90 kDa subunit isoform X1

    See identical proteins and their annotated locations for XP_006516323.1

    UniProtKB/Swiss-Prot
    Q8CFK2
    UniProtKB/TrEMBL
    G3X8S2
    Conserved Domains (2) summary
    COG1405
    Location:4 → 266
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:449 → 546
    BRF1; Brf1-like TBP-binding domain

  3. XM_030246945.2 → XP_030102805.1  transcription factor IIIB 90 kDa subunit isoform X6

    Conserved Domains (2) summary
    COG1405
    Location:1 → 62
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:244 → 341
    BRF1; Brf1-like TBP-binding domain

  4. XM_011244182.4 → XP_011242484.1  transcription factor IIIB 90 kDa subunit isoform X3

    See identical proteins and their annotated locations for XP_011242484.1

    Conserved Domains (2) summary
    COG1405
    Location:24 → 132
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:315 → 412
    BRF1; Brf1-like TBP-binding domain

  5. XM_030246944.2 → XP_030102804.1  transcription factor IIIB 90 kDa subunit isoform X5

    Conserved Domains (2) summary
    COG1405
    Location:1 → 62
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:245 → 342
    BRF1; Brf1-like TBP-binding domain

  6. XM_030246942.2 → XP_030102802.1  transcription factor IIIB 90 kDa subunit isoform X4

    Conserved Domains (2) summary
    COG1405
    Location:1 → 119
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:302 → 399
    BRF1; Brf1-like TBP-binding domain

  7. XM_011244185.4 → XP_011242487.1  transcription factor IIIB 90 kDa subunit isoform X5

    See identical proteins and their annotated locations for XP_011242487.1

    Conserved Domains (2) summary
    COG1405
    Location:1 → 62
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:245 → 342
    BRF1; Brf1-like TBP-binding domain

  8. XM_006516267.3 → XP_006516330.1  transcription factor IIIB 90 kDa subunit isoform X5

    See identical proteins and their annotated locations for XP_006516330.1

    Conserved Domains (2) summary
    COG1405
    Location:1 → 62
    SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]
    pfam07741
    Location:245 → 342
    BRF1; Brf1-like TBP-binding domain

RNA

  1. XR_872720.4 RNA Sequence

  2. XR_381545.5 RNA Sequence

  3. XR_001780489.3 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8CFK2.1 GenPept UniProtKB/Swiss-Prot:Q8CFK2

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