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Rnf135 ring finger protein 135 [ Mus musculus (house mouse) ]

Gene ID: 71956, updated on 5-Mar-2024

Summary

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Official Symbol
Rnf135provided by MGI
Official Full Name
ring finger protein 135provided by MGI
Primary source
MGI:MGI:1919206
See related
Ensembl:ENSMUSG00000020707 AllianceGenome:MGI:1919206
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Riplet; U 2-3-0; 0610037N03Rik; 2410006N06Rik
Summary
Predicted to enable several functions, including RIG-I binding activity; identical protein binding activity; and ubiquitin protein ligase activity. Involved in RIG-I signaling pathway; antiviral innate immune response; and protein polyubiquitination. Predicted to be located in cytoplasmic stress granule. Predicted to be part of ribonucleoprotein complex. Predicted to be active in cytoplasm. Is expressed in testis. Orthologous to human RNF135 (ring finger protein 135). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in ovary adult (RPKM 5.9), adrenal adult (RPKM 5.2) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
11 B5; 11 47.59 cM
Exon count:
5
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (80074652..80090581)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (80183869..80199755)

Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_30138 Neighboring gene transcription elongation factor, mitochondrial Neighboring gene ArfGAP with dual PH domains 2, opposite strand Neighboring gene ArfGAP with dual PH domains 2 Neighboring gene STARR-seq mESC enhancer starr_30143 Neighboring gene STARR-positive B cell enhancer ABC_E7025 Neighboring gene ras homolog family member T1 Neighboring gene STARR-seq mESC enhancer starr_30145 Neighboring gene STARR-positive B cell enhancer mm9_chr11:80116169-80116470 Neighboring gene rhomboid like 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. E3 Ubiquitin Ligase Riplet Is Expressed in T Cells and Suppresses T Cell-Mediated Antitumor Immune Responses. Iwamoto A, et al. J Immunol, 2022 Apr 15. PMID 35365564
  2. The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection. Oshiumi H, et al. Cell Host Microbe, 2010 Dec 16. PMID 21147464
  3. RIPLET, and not TRIM25, is required for endogenous RIG-I-dependent antiviral responses. Hayman TJ, et al. Immunol Cell Biol, 2019 Oct. PMID 31335993
  4. Altered levels of growth-related and novel gene transcripts in reproductive and other tissues of female mice overexpressing spermidine/spermine N1-acetyltransferase (SSAT). Min SH, et al. J Biol Chem, 2002 Feb 1. PMID 11709547
  5. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Shi Y, et al. Nat Commun, 2017 May 4. PMID 28469175, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. E3 Ubiquitin Ligase Riplet Is Expressed in T Cells and Suppresses T Cell-Mediated Antitumor Immune Responses.
    Title: E3 Ubiquitin Ligase Riplet Is Expressed in T Cells and Suppresses T Cell-Mediated Antitumor Immune Responses.
  2. Riplet, and not TRIM25, is required endogenously for the ubiquitination of RIG-I.
    Title: RIPLET, and not TRIM25, is required for endogenous RIG-I-dependent antiviral responses.
  3. Ubiquitin-Dependent and -Independent Roles of E3 Ligase RIPLET in Innate Immunity.
    Title: Ubiquitin-Dependent and -Independent Roles of E3 Ligase RIPLET in Innate Immunity.
  4. Together with Riplet, Ube2D3 promotes covalent conjugation of polyubiquitin chains to RIG-I, while Ube2N preferentially facilitates production of unanchored chains. In the presence of these chains, RIG-I induces MAVS aggregation directly on the mitochondria. Data thus reveal two essential mechanisms underlying the activation of RIG-I and MAVS for triggering innate immune signaling in response to viral infection in cells.
    Title: Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.
  5. Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein.
    Title: A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.
  6. Riplet is essential for regulating RIG-I-mediated innate immune response against RNA virus infection in vivo.
    Title: The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (5) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC13061

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RIG-I binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribonucleoprotein complex binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribonucleoprotein complex binding ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin-protein transferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in RIG-I signaling pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in RIG-I signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in RIG-I signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in antiviral innate immune response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in antiviral innate immune response ISO
Inferred from Sequence Orthology
more info
  
involved_in free ubiquitin chain polymerization ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within immune system process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within innate immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of interferon-beta production ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K63-linked ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein homooligomerization ISO
Inferred from Sequence Orthology
more info
  
involved_in protein polyubiquitination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein polyubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of innate immune response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of innate immune response ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasmic stress granule ISO
Inferred from Sequence Orthology
more info
  
part_of ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase RNF135
Names
RING-type E3 ubiquitin transferase RNF135
NP_082295.1
XP_036012897.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_028019.4 → NP_082295.1  E3 ubiquitin-protein ligase RNF135

    See identical proteins and their annotated locations for NP_082295.1

    Status: VALIDATED

    Source sequence(s)
    AL591426
    Consensus CDS
    CCDS25129.1
    UniProtKB/Swiss-Prot
    Q3UBK5, Q8R161, Q9CWS1, Q9DCH3
    UniProtKB/TrEMBL
    B2RRA5
    Related
    ENSMUSP00000017839.3, ENSMUST00000017839.3
    Conserved Domains (2) summary
    cd00162
    Location:21 → 68
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd12902
    Location:245 → 413
    SPRY_PRY_RNF135; PRY/SPRY domain in RING finger protein RNF135

RNA

  1. NR_157298.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AK010429, AL591426, BC145236, BY754501

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000077.7 Reference GRCm39 C57BL/6J

    Range
    80074652..80090581
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036157004.1 → XP_036012897.1  E3 ubiquitin-protein ligase RNF135 isoform X1

    Conserved Domains (4) summary
    TIGR00599
    Location:16 → 140
    rad18; DNA repair protein rad18
    cd12902
    Location:197 → 365
    SPRY_PRY_RNF135; PRY/SPRY domain in RING finger protein RNF135
    cd00162
    Location:21 → 66
    RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]
    cl17238
    Location:21 → 67
    RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9CWS1.1 GenPept UniProtKB/Swiss-Prot:Q9CWS1

Gene LinkOut

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