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Nadk2 NAD kinase 2, mitochondrial [ Mus musculus (house mouse) ]

Gene ID: 68646, updated on 21-Apr-2024

Summary

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Official Symbol
Nadk2provided by MGI
Official Full Name
NAD kinase 2, mitochondrialprovided by MGI
Primary source
MGI:MGI:1915896
See related
Ensembl:ENSMUSG00000022253 AllianceGenome:MGI:1915896
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
MNADK; Nadkd1
Summary
Predicted to enable NAD+ kinase activity and protein homodimerization activity. Predicted to be involved in NAD metabolic process. Located in mitochondrion. Is expressed in retina. Orthologous to human NADK2 (NAD kinase 2, mitochondrial). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in liver E14 (RPKM 40.2), liver E14.5 (RPKM 34.4) and 23 other tissues See more
Orthologs
human all
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Genomic context

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Location:
15 A1; 15 3.82 cM
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (9071340..9110584)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (9071260..9110496)

Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene RAN binding protein 3-like Neighboring gene predicted gene, 53883 Neighboring gene STARR-positive B cell enhancer ABC_E1904 Neighboring gene STARR-seq mESC enhancer starr_37924 Neighboring gene STARR-positive B cell enhancer ABC_E3131 Neighboring gene S-phase kinase-associated protein 2 Neighboring gene LMBR1 domain containing 2 Neighboring gene STARR-seq mESC enhancer starr_37931 Neighboring gene STARR-seq mESC enhancer starr_37932 Neighboring gene UDP glycosyltransferases 3 family pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Deficiency of the Mitochondrial NAD Kinase Causes Stress-Induced Hepatic Steatosis in Mice. Zhang K, et al. Gastroenterology, 2018 Jan. PMID 28923496, Free PMC Article
  2. [Coxsackie virus B3 (CVB3) triggers the activation of NLRP3 in mouse macrophages by activating nicotinamide adenine dinucleotide kinase 2 (NADK2)]. Sang R, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2023 Jan. PMID 36631013
  3. The mitochondrial NAD kinase functions as a major metabolic regulator upon increased energy demand. Kim H, et al. Mol Metab, 2022 Oct. PMID 35944895, Free PMC Article
  4. Mouse models of NADK2 deficiency analyzed for metabolic and gene expression changes to elucidate pathophysiology. Murray GC, et al. Hum Mol Genet, 2022 Nov 28. PMID 35796562, Free PMC Article
  5. MNADK, a novel liver-enriched mitochondrion-localized NAD kinase. Zhang R. Biol Open, 2013 Apr 15. PMID 23616928, Free PMC Article

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. [Coxsackie virus B3 (CVB3) triggers the activation of NLRP3 in mouse macrophages by activating nicotinamide adenine dinucleotide kinase 2 (NADK2)].
    Title: [Coxsackie virus B3 (CVB3) triggers the activation of NLRP3 in mouse macrophages by activating nicotinamide adenine dinucleotide kinase 2 (NADK2)].
  2. Mouse models of NADK2 deficiency analyzed for metabolic and gene expression changes to elucidate pathophysiology.
    Title: Mouse models of NADK2 deficiency analyzed for metabolic and gene expression changes to elucidate pathophysiology.
  3. Deleting MNADK (NADK2) in mice reduced fat oxidation upon energy demand triggered by endurance exercise or fasting. NADK2 is a major metabolic regulator upon increased energy demand.
    Title: The mitochondrial NAD kinase functions as a major metabolic regulator upon increased energy demand.
  4. MNADK is localized to mitochondria in Hep G2 cells, and a recombinant MNADK showed NAD kinase activity. MNADK is abundant in mitochondrion-rich tissues, such as liver. MNADK expression was suppressed in human liver tumors. Identification of MNADK immediately suggests a model in which NADK and MNADK are responsible for de novo synthesis of NADP(+) in cytosol and mitochondria, respectively.
    Title: MNADK, a novel liver-enriched mitochondrion-localized NAD kinase.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2) 
  • Chemically induced (ENU) (2)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • 1110020G09Rik, 4933430B08Rik

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables NAD+ kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables NAD+ kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in NAD metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in NAD metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in NADP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
NAD kinase 2, mitochondrial
Names
NAD kinase domain-containing protein 1, mitochondrial
NP_001035485.2
NP_001078879.1
NP_001273182.1
NP_001273184.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001040395.4 → NP_001035485.2  NAD kinase 2, mitochondrial isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.
    Source sequence(s)
    AK046666, AK078318, AK162601, AW537715, BE692075, BY545448
    UniProtKB/TrEMBL
    Q14BL1
    Conserved Domains (1) summary
    cl01255
    Location:84 → 306
    DAGK_cat; Diacylglycerol kinase catalytic domain

  2. NM_001085410.2 → NP_001078879.1  NAD kinase 2, mitochondrial isoform 1

    See identical proteins and their annotated locations for NP_001078879.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AK046666, AK078318, AK162601, AW537715, BE692075, BY545448
    UniProtKB/Swiss-Prot
    Q3TRP5, Q3UEY1, Q8C5H8, Q9CTE4
    UniProtKB/TrEMBL
    Q14BL1
    Related
    ENSMUSP00000159405.2, ENSMUST00000190591.10
    Conserved Domains (1) summary
    cl01255
    Location:84 → 328
    DAGK_cat; Diacylglycerol kinase catalytic domain

  3. NM_001286253.1 → NP_001273182.1  NAD kinase 2, mitochondrial isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, lacks several additional exons, and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AK016988, AK046666, BF460794
    UniProtKB/TrEMBL
    A0A7P0A156
    Related
    ENSMUSP00000159408.2, ENSMUST00000190874.8
    Conserved Domains (1) summary
    cl01255
    Location:84 → 306
    DAGK_cat; Diacylglycerol kinase catalytic domain

  4. NM_001286255.1 → NP_001273184.1  NAD kinase 2, mitochondrial isoform 4

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks two in-frame exons in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1.
    Source sequence(s)
    AK046666, AK078318, AK162601, AW537715, BE692075, BY545448
    UniProtKB/TrEMBL
    A0A7N9VS49
    Related
    ENSMUSP00000159407.2, ENSMUST00000189437.8
    Conserved Domains (1) summary
    cl01255
    Location:84 → 278
    DAGK_cat; Diacylglycerol kinase catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000081.7 Reference GRCm39 C57BL/6J

    Range
    9071340..9110584
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8C5H8.2 GenPept UniProtKB/Swiss-Prot:Q8C5H8

Gene LinkOut

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