Gpsm1 G-protein signalling modulator 1 (AGS3-like, C. elegans) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gpsm1provided by MGI
Official Full Name: G-protein signalling modulator 1 (AGS3-like, C. elegans)provided by MGI
Primary source: MGI:MGI:1915089
See related: Ensembl:ENSMUSG00000026930 AllianceGenome:MGI:1915089
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ags3; 1810037C22Rik
Summary: Predicted to enable G-protein alpha-subunit binding activity and GDP-dissociation inhibitor activity. Predicted to be involved in positive regulation of macroautophagy; regulation of G protein-coupled receptor signaling pathway; and regulation of GTPase activity. Predicted to act upstream of or within cell differentiation and nervous system development. Predicted to be located in plasma membrane. Predicted to be part of protein-containing complex. Predicted to colocalize with Golgi membrane; cell cortex; and endoplasmic reticulum membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; integumental system; and peripheral nervous system ganglion. Orthologous to human GPSM1 (G protein signaling modulator 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in ovary adult (RPKM 24.9), whole brain E14.5 (RPKM 24.0) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 A3; 2 18.73 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (26204623..26238249)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (26314611..26348237)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity.
Title: GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity.
AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.
Title: AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis.
GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages.
Title: GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages.
AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
Title: AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
Genome-wide meta-analysis associates GPSM1 with type 2 diabetes, a plausible gene involved in skeletal muscle function.
Title: Genome-wide meta-analysis associates GPSM1 with type 2 diabetes, a plausible gene involved in skeletal muscle function.
findings show that AGS3 is not required for planar divisions of neural progenitors in the mouse neocortex. AGS3 is not recruited to the cell cortex and does not rescue LGN loss of function.
Title: Loss of the canonical spindle orientation function in the Pins/LGN homolog AGS3.
We conclude that LPS priming increases AGS3 levels, which enhances lysosomal function and increases the capacity of macrophages to eliminate intracellular pathogens.
Title: Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection.
level of basal autophagy, autophagic induction, autophagic flux, autophagic degradation and the anti-autophagic action in macrophages that lacked Galphai3, AGS3, or RGS19; or had been treated with pertussis toxin, were similar to controls
Title: Normal autophagic activity in macrophages from mice lacking Gαi3, AGS3, or RGS19.
AGS3 has a role in the regulation of G-protein signaling in the immune system
Title: Defective chemokine signal integration in leukocytes lacking activator of G protein signaling 3 (AGS3).
these results indicate that GRK6 complexes with AGS3-Galphai2 to regulate CXCR2-mediated leukocyte functions at different levels, including downstream effector activation, receptor trafficking, and expression at the cell membrane.
Title: G Protein-coupled receptor kinase-6 interacts with activator of G protein signaling-3 to regulate CXCR2-mediated cellular functions.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234352

AW107933 (e-PCR)
- UniSTS:178594

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G-protein alpha-subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein alpha-subunit binding	ISO Inferred from Sequence Orthology more info
enables GDP-dissociation inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables GDP-dissociation inhibitor activity	ISO Inferred from Sequence Orthology more info
enables GTPase regulator activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of macroautophagy	ISO Inferred from Sequence Orthology more info
involved_in regulation of G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
colocalizes_with Golgi membrane	ISO Inferred from Sequence Orthology more info
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with cell cortex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
colocalizes_with endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: G-protein-signaling modulator 1

Names: activator of G-protein signaling 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199146.1 → NP_001186075.1 G-protein-signaling modulator 1 isoform 2

See identical proteins and their annotated locations for NP_001186075.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate 5' terminus which results in the use of an alternate tranlation initiation site and lacks an in-frame exon at the 3' end, compared to variant 3. Variant 2 encodes isoform 2 which has a shorter and distinct N-terminus, compared to isoform 3.

Source sequence(s)

AK084631, BC071197, CA317518

Consensus CDS

CCDS57158.1

UniProtKB/Swiss-Prot

Q6IR34

Related

ENSMUSP00000077686.6, ENSMUST00000078616.12

Conserved Domains (6) summary

smart00390
Location:534 → 556

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

sd00006
Location:207 → 234

TPR; TPR repeat [structural motif]

pfam00515
Location:106 → 138

TPR_1; Tetratricopeptide repeat

pfam02188
Location:434 → 455

GoLoco; GoLoco motif

pfam13176
Location:288 → 322

TPR_7; Tetratricopeptide repeat

pfam13424
Location:207 → 278

TPR_12; Tetratricopeptide repeat
NM_001199147.1 → NP_001186076.1 G-protein-signaling modulator 1 isoform 3

See identical proteins and their annotated locations for NP_001186076.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3).

Source sequence(s)

BC071197, CK618701

Consensus CDS

CCDS57159.1

UniProtKB/Swiss-Prot

Q6IR34

Related

ENSMUSP00000067964.7, ENSMUST00000066889.13

Conserved Domains (6) summary

smart00390
Location:626 → 648

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

sd00006
Location:239 → 266

TPR; TPR repeat [structural motif]

pfam00515
Location:138 → 170

TPR_1; Tetratricopeptide repeat

pfam02188
Location:526 → 547

GoLoco; GoLoco motif

pfam13176
Location:320 → 354

TPR_7; Tetratricopeptide repeat

pfam13424
Location:239 → 310

TPR_12; Tetratricopeptide repeat
NM_001355574.1 → NP_001342503.1 G-protein-signaling modulator 1 isoform 4

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AL732541

Conserved Domains (5) summary

smart00390
Location:571 → 593

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

COG0457
Location:37 → 335

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:184 → 211

TPR; TPR repeat [structural motif]

pfam02188
Location:471 → 492

GoLoco; GoLoco motif

pfam13424
Location:184 → 255

TPR_12; Tetratricopeptide repeat
NM_001355575.1 → NP_001342504.1 G-protein-signaling modulator 1 isoform 5

Status: VALIDATED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence and uses an alternate splice junction compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

AL732541

Related

ENSMUSP00000115680.2, ENSMUST00000145884.8

Conserved Domains (4) summary

COG0457
Location:66 → 237

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:86 → 113

TPR; TPR repeat [structural motif]

pfam02188
Location:373 → 394

GoLoco; GoLoco motif

pfam13424
Location:86 → 157

TPR_12; Tetratricopeptide repeat
NM_001355576.1 → NP_001342505.1 G-protein-signaling modulator 1 isoform 6

Status: VALIDATED

Description

Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (6) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1.

Source sequence(s)

AL732541

Conserved Domains (5) summary

smart00390
Location:511 → 533

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

COG0457
Location:27 → 335

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:184 → 211

TPR; TPR repeat [structural motif]

pfam02188
Location:411 → 432

GoLoco; GoLoco motif

pfam13424
Location:184 → 255

TPR_12; Tetratricopeptide repeat
NM_153410.5 → NP_700459.2 G-protein-signaling modulator 1 isoform 1

See identical proteins and their annotated locations for NP_700459.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) has an alternate 5' terminus which results in the use of an alternate tranlation initiation site, compared to variant 3. Variant 1 encodes isoform 1 which has a shorter and distinct N-terminus, compared to isoform 3.

Source sequence(s)

AK048691, BC071197, CA317518

Consensus CDS

CCDS15800.1

UniProtKB/Swiss-Prot

A2AIX7, A2AIX8, A2AIX9, Q61366, Q6IR34, Q8BUK4, Q8BX78, Q8R0R9

Related

ENSMUSP00000065000.3, ENSMUST00000066936.9

Conserved Domains (5) summary

smart00390
Location:594 → 616

GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

COG0457
Location:60 → 358

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:207 → 234

TPR; TPR repeat [structural motif]

pfam13424
Location:207 → 278

TPR_12; Tetratricopeptide repeat

pfam02188
Location:494 → 513

GoLoco; GoLoco motif

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

26204623..26238249

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036162509.1 → XP_036018402.1 G-protein-signaling modulator 1 isoform X2

UniProtKB/TrEMBL

A2AIV3

Related

ENSMUSP00000109769.3, ENSMUST00000114134.9

Conserved Domains (1) summary

pfam02188
Location:57 → 76

GoLoco; GoLoco motif
XM_036162508.1 → XP_036018401.1 G-protein-signaling modulator 1 isoform X1

Conserved Domains (1) summary

pfam02188
Location:117 → 136

GoLoco; GoLoco motif
XM_006498255.3 → XP_006498318.1 G-protein-signaling modulator 1 isoform X3

Conserved Domains (1) summary

pfam02188
Location:41 → 60

GoLoco; GoLoco motif