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Rnf220 ring finger protein 220 [ Mus musculus (house mouse) ]

Gene ID: 66743, updated on 21-Apr-2024

Summary

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Official Symbol
Rnf220provided by MGI
Official Full Name
ring finger protein 220provided by MGI
Primary source
MGI:MGI:1913993
See related
Ensembl:ENSMUSG00000028677 AllianceGenome:MGI:1913993
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
4732477A13; 4931406I20Rik; 5730503K05Rik
Summary
Enables ubiquitin protein ligase activity. Involved in protein autoubiquitination. Acts upstream of or within several processes, including nervous system development; positive regulation of DNA-binding transcription factor activity; and protein monoubiquitination. Located in cytoplasm. Is expressed in several structures, including central nervous system; dorsal root ganglion; genitourinary system; retina; and tooth. Orthologous to human RNF220 (ring finger protein 220). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in testis adult (RPKM 15.8), ovary adult (RPKM 14.8) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
4 53.44 cM; 4 D1
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (117128660..117354249, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (117271463..117497052, complement)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 23287 Neighboring gene CapStarr-seq enhancer MGSCv37_chr4:116923419-116923602 Neighboring gene armadillo-like helical domain containing 1 Neighboring gene STARR-positive B cell enhancer ABC_E7982 Neighboring gene STARR-seq mESC enhancer starr_11163 Neighboring gene transmembrane protein 53 Neighboring gene predicted gene 12843 Neighboring gene predicted gene, 40231 Neighboring gene STARR-seq mESC enhancer starr_11170 Neighboring gene STARR-seq mESC enhancer starr_11172 Neighboring gene STARR-seq mESC enhancer starr_11174 Neighboring gene STARR-seq mESC enhancer starr_11177 Neighboring gene STARR-seq mESC enhancer starr_11178 Neighboring gene STARR-seq mESC enhancer starr_11179 Neighboring gene STARR-seq mESC enhancer starr_11180 Neighboring gene predicted gene 12828 Neighboring gene predicted gene 12827 Neighboring gene predicted gene, 34669 Neighboring gene STARR-positive B cell enhancer mm9_chr4:117222596-117222897 Neighboring gene STARR-seq mESC enhancer starr_11185 Neighboring gene exoribonuclease 3 Neighboring gene zinc finger (CCCH type), RNA binding motif and serine/arginine rich 2 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Haploinsufficiency of the TDP43 ubiquitin E3 ligase RNF220 leads to ALS-like motor neuron defects in the mouse. Ma P, et al. J Mol Cell Biol, 2021 Aug 18. PMID 33386850, Free PMC Article
  2. Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation. Zhang L, et al. Cells, 2020 Jul 1. PMID 32630355, Free PMC Article
  3. RNF220 is required for cerebellum development and regulates medulloblastoma progression through epigenetic modulation of Shh signaling. Ma P, et al. Development, 2020 Jun 15. PMID 32376680
  4. RNF220 mediates K63-linked polyubiquitination of STAT1 and promotes host defense. Guo X, et al. Cell Death Differ, 2021 Feb. PMID 32814877, Free PMC Article
  5. RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission. Ma P, et al. Sci Adv, 2022 Sep 30. PMID 36179027, Free PMC Article

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
    Title: RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination.
  2. RNF220 promotes gastric cancer growth and stemness via modulating the USP22/wnt/beta-catenin pathway.
    Title: RNF220 promotes gastric cancer growth and stemness via modulating the USP22/wnt/β-catenin pathway.
  3. Haploinsufficiency of the TDP43 ubiquitin E3 ligase RNF220 leads to ALS-like motor neuron defects in the mouse.
    Title: Haploinsufficiency of the TDP43 ubiquitin E3 ligase RNF220 leads to ALS-like motor neuron defects in the mouse.
  4. RNF220 mediates K63-linked polyubiquitination of STAT1 and promotes host defense.
    Title: RNF220 mediates K63-linked polyubiquitination of STAT1 and promotes host defense.
  5. RNF220-mediated ubiquitination promotes aggresomal accumulation and autophagic degradation of cytoplasmic Gli via HDAC6.
    Title: RNF220-mediated ubiquitination promotes aggresomal accumulation and autophagic degradation of cytoplasmic Gli via HDAC6.
  6. ZC4H2 stabilizes RNF220 to pattern ventral spinal cord through modulating Shh/Gli signaling.
    Title: ZC4H2 stabilizes RNF220 to pattern ventral spinal cord through modulating Shh/Gli signaling.
  7. Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation.
    Title: Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation.
  8. RNF220 is required for cerebellum development and regulates medulloblastoma progression through epigenetic modulation of Shh signaling.
    Title: RNF220 is required for cerebellum development and regulates medulloblastoma progression through epigenetic modulation of Shh signaling.
  9. Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development.
    Title: Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development.
  10. RNF220 plays crucial roles in the patterning of the ventral progenitor domains, by targeting multiple transcription factors, including Dbx1/2 and Nkx2.2, for degradation in close cooperation with ZC4H2.
    Title: Rnf220 cooperates with Zc4h2 to specify spinal progenitor domains.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables beta-catenin binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within dorsal/ventral neural tube patterning IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within noradrenergic neuron development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of DNA-binding transcription factor activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within positive regulation of DNA-binding transcription factor activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in positive regulation of canonical Wnt signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in protein autoubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein monoubiquitination IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within protein monoubiquitination ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of postsynaptic neurotransmitter receptor internalization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of postsynaptic neurotransmitter receptor internalization IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in regulation of postsynaptic neurotransmitter receptor internalization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within regulation of transcription regulatory region DNA binding IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within regulation of transcription regulatory region DNA binding ISO
Inferred from Sequence Orthology
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in glutamatergic synapse IEP
Inferred from Expression Pattern
more info
 PubMed 
is_active_in glutamatergic synapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in nuclear lamina ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
is_active_in postsynapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in postsynapse IEP
Inferred from Expression Pattern
more info
 PubMed 
is_active_in postsynapse IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of protein-containing complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase Rnf220
Names
RING-type E3 ubiquitin transferase Rnf220
NP_001297658.1
NP_001297659.1
NP_001297660.1
NP_080015.3
XP_006503348.1
XP_011238889.1
XP_011238890.1
XP_011238891.1
XP_017175845.1
XP_017175846.1
XP_030109563.1
XP_030109564.1
XP_036020239.1
XP_036020240.1
XP_036020241.1
XP_036020242.1
XP_036020243.1
XP_036020244.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001310729.1 → NP_001297658.1  E3 ubiquitin-protein ligase Rnf220 isoform 2

    See identical proteins and their annotated locations for NP_001297658.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in its 5' UTR, lacks a portion of the 5' coding region, initiates translation at a downstream start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) has a significantly shorter N-terminus, but contains an alternate internal protein segment compared to isoform 1.
    Source sequence(s)
    AI837557, BC056359, BY716092, CA463153
    Consensus CDS
    CCDS80150.1
    UniProtKB/TrEMBL
    Q9D4L6
    Related
    ENSMUSP00000092449.3, ENSMUST00000094853.9
    Conserved Domains (2) summary
    pfam15926
    Location:1 → 229
    RNF220; E3 ubiquitin-protein ligase RNF220
    cd16563
    Location:300 → 340
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins

  2. NM_001310730.1 → NP_001297659.1  E3 ubiquitin-protein ligase Rnf220 isoform 3

    See identical proteins and their annotated locations for NP_001297659.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in its 5' UTR, lacks a portion of the 5' coding region, initiates translation at an alternate start codon, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) has a distinct and significantly shorter N-terminus, but contains an alternate internal protein segment compared to isoform 1.
    Source sequence(s)
    AI837557, AK016436, AL844169, BC056359, CJ105892
    Consensus CDS
    CCDS89815.1
    UniProtKB/Swiss-Prot
    Q6PDX6
    UniProtKB/TrEMBL
    Q9D4L6
    Related
    ENSMUSP00000152367.2, ENSMUST00000221654.2
    Conserved Domains (2) summary
    pfam13923
    Location:256 → 294
    zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)
    pfam15926
    Location:14 → 184
    RNF220; E3 ubiquitin-protein ligase RNF220

  3. NM_001310731.1 → NP_001297660.1  E3 ubiquitin-protein ligase Rnf220 isoform 4

    See identical proteins and their annotated locations for NP_001297660.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in its 5' UTR, lacks a portion of the 5' coding region, initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (4) has a significantly shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AI837557, AK016436, AL844169, BC056359, BE953159, CJ105892
    Consensus CDS
    CCDS80149.1
    UniProtKB/Swiss-Prot
    Q6PDX6
    UniProtKB/TrEMBL
    Q9D4L6
    Conserved Domains (2) summary
    cd16563
    Location:192 → 232
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:11 → 121
    RNF220; E3 ubiquitin-protein ligase RNF220

  4. NM_025739.3 → NP_080015.3  E3 ubiquitin-protein ligase Rnf220 isoform 1

    See identical proteins and their annotated locations for NP_080015.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AI837557, AK041785, BC056359
    Consensus CDS
    CCDS18534.1
    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Related
    ENSMUSP00000030439.9, ENSMUST00000030439.15
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    117128660..117354249 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503285.3 → XP_006503348.1  E3 ubiquitin-protein ligase Rnf220 isoform X1

    See identical proteins and their annotated locations for XP_006503348.1

    Conserved Domains (2) summary
    cd16563
    Location:539 → 579
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 468
    RNF220; E3 ubiquitin-protein ligase RNF220

  2. XM_036164349.1 → XP_036020242.1  E3 ubiquitin-protein ligase Rnf220 isoform X2

    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

  3. XM_036164348.1 → XP_036020241.1  E3 ubiquitin-protein ligase Rnf220 isoform X2

    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

  4. XM_036164350.1 → XP_036020243.1  E3 ubiquitin-protein ligase Rnf220 isoform X2

    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

  5. XM_011240588.3 → XP_011238890.1  E3 ubiquitin-protein ligase Rnf220 isoform X1

    See identical proteins and their annotated locations for XP_011238890.1

    Conserved Domains (2) summary
    cd16563
    Location:539 → 579
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 468
    RNF220; E3 ubiquitin-protein ligase RNF220

  6. XM_036164347.1 → XP_036020240.1  E3 ubiquitin-protein ligase Rnf220 isoform X2

    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

  7. XM_036164346.1 → XP_036020239.1  E3 ubiquitin-protein ligase Rnf220 isoform X2

    UniProtKB/Swiss-Prot
    A2APP0, Q3T9M4, Q3V3D7, Q6PDX6, Q8JZY5
    Conserved Domains (2) summary
    cd16563
    Location:513 → 553
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 442
    RNF220; E3 ubiquitin-protein ligase RNF220

  8. XM_011240587.4 → XP_011238889.1  E3 ubiquitin-protein ligase Rnf220 isoform X1

    See identical proteins and their annotated locations for XP_011238889.1

    Conserved Domains (2) summary
    cd16563
    Location:539 → 579
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:219 → 468
    RNF220; E3 ubiquitin-protein ligase RNF220

  9. XM_036164351.1 → XP_036020244.1  E3 ubiquitin-protein ligase Rnf220 isoform X3

    Conserved Domains (2) summary
    cd16563
    Location:274 → 314
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:1 → 203
    RNF220; E3 ubiquitin-protein ligase RNF220

  10. XM_011240589.4 → XP_011238891.1  E3 ubiquitin-protein ligase Rnf220 isoform X5

    See identical proteins and their annotated locations for XP_011238891.1

    UniProtKB/Swiss-Prot
    Q6PDX6
    Conserved Domains (2) summary
    cd16563
    Location:192 → 232
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:11 → 121
    RNF220; E3 ubiquitin-protein ligase RNF220

  11. XM_017320356.3 → XP_017175845.1  E3 ubiquitin-protein ligase Rnf220 isoform X4

    Conserved Domains (2) summary
    cd16563
    Location:229 → 269
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:14 → 158
    RNF220; E3 ubiquitin-protein ligase RNF220

  12. XM_030253704.2 → XP_030109564.1  E3 ubiquitin-protein ligase Rnf220 isoform X5

    Related
    ENSMUSP00000099751.3, ENSMUST00000102690.9
    Conserved Domains (2) summary
    cd16563
    Location:192 → 232
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:11 → 121
    RNF220; E3 ubiquitin-protein ligase RNF220

  13. XM_030253703.2 → XP_030109563.1  E3 ubiquitin-protein ligase Rnf220 isoform X5

    Conserved Domains (2) summary
    cd16563
    Location:192 → 232
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:11 → 121
    RNF220; E3 ubiquitin-protein ligase RNF220

  14. XM_017320357.3 → XP_017175846.1  E3 ubiquitin-protein ligase Rnf220 isoform X5

    UniProtKB/Swiss-Prot
    Q6PDX6
    Conserved Domains (2) summary
    cd16563
    Location:192 → 232
    RING-HC_RNF220; RING finger, HC subclass, found in RING finger protein 220 (RNF220) and similar proteins
    pfam15926
    Location:11 → 121
    RNF220; E3 ubiquitin-protein ligase RNF220

RNA

  1. XR_004941948.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6PDX6.1 GenPept UniProtKB/Swiss-Prot:Q6PDX6

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