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Spag16 sperm associated antigen 16 [ Mus musculus (house mouse) ]

Gene ID: 66722, updated on 5-Mar-2024

Summary

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Official Symbol
Spag16provided by MGI
Official Full Name
sperm associated antigen 16provided by MGI
Primary source
MGI:MGI:1913972
See related
Ensembl:ENSMUSG00000053153 AllianceGenome:MGI:1913972
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Pf20; Wdr29; 4921511D23Rik; 4930524F24Rik; 4930585K05Rik
Summary
Enables protein kinase binding activity. Involved in cilium assembly. Acts upstream of or within several processes, including cell motility in response to calcium ion; epithelial cilium movement involved in extracellular fluid movement; and sperm axoneme assembly. Located in cytoskeleton; nucleus; and sperm flagellum. Orthologous to human SPAG16 (sperm associated antigen 16). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in testis adult (RPKM 9.4), lung adult (RPKM 1.1) and 1 other tissue See more
Orthologs
human all
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Genomic context

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Location:
1 C3; 1 35.04 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (69866031..70764291)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (69826872..70725132)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene IKAROS family zinc finger 2 Neighboring gene STARR-seq mESC enhancer starr_01218 Neighboring gene STARR-seq mESC enhancer starr_01219 Neighboring gene STARR-seq mESC enhancer starr_01220 Neighboring gene predicted gene, 53559 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:69815414-69815635 Neighboring gene predicted gene, 29822 Neighboring gene STARR-seq mESC enhancer starr_01224 Neighboring gene STARR-seq mESC enhancer starr_01225 Neighboring gene STARR-seq mESC enhancer starr_01226 Neighboring gene predicted gene, 23879 Neighboring gene STARR-seq mESC enhancer starr_01227 Neighboring gene STARR-seq mESC enhancer starr_01228 Neighboring gene STARR-seq mESC enhancer starr_01229 Neighboring gene predicted gene, 17972 Neighboring gene predicted gene, 23422 Neighboring gene predicted gene, 57571 Neighboring gene von Willebrand factor C domain-containing protein 2-like Neighboring gene STARR-seq mESC enhancer starr_01230 Neighboring gene predicted gene, 29906 Neighboring gene predicted gene, 57799 Neighboring gene ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Spag16, an axonemal central apparatus gene, encodes a male germ cell nuclear speckle protein that regulates SPAG16 mRNA expression. Nagarkatti-Gude DR, et al. PLoS One, 2011. PMID 21655194, Free PMC Article
  2. Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues. Alciaturi J, et al. J Mol Histol, 2019 Jun. PMID 30911868
  3. Elongin B is a binding partner of the male germ cell nuclear speckle protein sperm-associated antigen 16S (SPAG16S) and is regulated post-transcriptionally in the testis. Zhang Z, et al. Reprod Fertil Dev, 2019 Apr. PMID 30811962, Free PMC Article
  4. Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L. Lesich KA, et al. Biol Reprod, 2010 Apr. PMID 20042536, Free PMC Article
  5. Accelerated mortality from hydrocephalus and pneumonia in mice with a combined deficiency of SPAG6 and SPAG16L reveals a functional interrelationship between the two central apparatus proteins. Zhang Z, et al. Cell Motil Cytoskeleton, 2007 May. PMID 17323374

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In this work, we performed a quantitative analysis of the expression of Spag6 and Spag16 genes in parallel with the immune-localization of the proteins in several tissues of adult mice.
    Title: Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues.
  2. The distinct nuclear localization of SPAG16S and its ability to modulate Spag16L mRNA expression suggest that SPAG16S plays an important role in the gene expression machinery of male germ cells.
    Title: Spag16, an axonemal central apparatus gene, encodes a male germ cell nuclear speckle protein that regulates SPAG16 mRNA expression.
  3. Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L.
    Title: Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L.
  4. SPAG16L can be phosphorylated by TSSK2 in vitro.
    Title: Phosphorylation of mouse sperm axoneme central apparatus protein SPAG16L by a testis-specific kinase, TSSK2.
  5. In sperm, SPAG16L is found in the central apparatus of the axoneme. We conclude that SPAG16L is essential for sperm flagellar function.
    Title: Deficiency of SPAG16L causes male infertility associated with impaired sperm motility.
  6. Data describe the cloning of human and mouse Pf20 proteins that interact with Spag6 protein.
    Title: A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20 associates with Spag6, the mammalian orthologue of Chlamydomonas PF16.
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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in axoneme assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in axoneme assembly ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within cell projection organization IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cerebrospinal fluid circulation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in cilium assembly IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within mucociliary clearance IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within sperm axoneme assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in axonemal central apparatus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in axonemal central apparatus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in axoneme IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell projection IEA
Inferred from Electronic Annotation
more info
  
located_in cilium IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in motile cilium IEA
Inferred from Electronic Annotation
more info
  
located_in sperm flagellum IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
sperm-associated antigen 16 protein
Names
pf20 protein homolog
sperm-associated WD repeat protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001271533.1 → NP_001258462.1  sperm-associated antigen 16 protein isoform 3

    See identical proteins and their annotated locations for NP_001258462.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks several exons and includes an alternate 3' terminal exon compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus compared to isoform 1.
    Source sequence(s)
    AA103272, AC167331, AK083083
    Consensus CDS
    CCDS69901.1
    UniProtKB/Swiss-Prot
    Q8K450
    Related
    ENSMUSP00000109573.3, ENSMUST00000113940.4

  2. NM_025728.4 → NP_080004.1  sperm-associated antigen 16 protein isoform 2

    See identical proteins and their annotated locations for NP_080004.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains an alternate 5'-most exon in the 5' UTR and lacks a portion of the 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2; also known as Spag16S) with a shorter N-terminus, compared to isoform 1.
    Source sequence(s)
    AC129933, AK014865, CJ046986
    UniProtKB/Swiss-Prot
    Q8K450
    Related
    ENSMUST00000161310.8
    Conserved Domains (3) summary
    COG2319
    Location:20 → 309
    WD40; WD40 repeat [General function prediction only]
    cd00200
    Location:23 → 309
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    sd00039
    Location:34 → 71
    7WD40; WD40 repeat [structural motif]

  3. NM_029160.3 → NP_083436.2  sperm-associated antigen 16 protein isoform 1

    See identical proteins and their annotated locations for NP_083436.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1; also known as Spag16L).
    Source sequence(s)
    AC101802, AC102458, AC102666, AC129933, AC164411, AC167331
    Consensus CDS
    CCDS15027.1
    UniProtKB/Swiss-Prot
    Q0VBE7, Q8C450, Q8K450, Q9CPU8, Q9D522
    Related
    ENSMUSP00000069821.6, ENSMUST00000065425.12
    Conserved Domains (3) summary
    COG2319
    Location:349 → 638
    WD40; WD40 repeat [General function prediction only]
    cd00200
    Location:352 → 638
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    sd00039
    Location:363 → 400
    7WD40; WD40 repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    69866031..70764291
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006496194.3 → XP_006496257.1  sperm-associated antigen 16 protein isoform X1

    Conserved Domains (1) summary
    pfam18654
    Location:144 → 232
    LegC3_N; LegC3 N-terminal coiled-coil domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8K450.1 GenPept UniProtKB/Swiss-Prot:Q8K450

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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