Iscu iron-sulfur cluster assembly enzyme [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Iscuprovided by MGI
Official Full Name: iron-sulfur cluster assembly enzymeprovided by MGI
Primary source: MGI:MGI:1913633
See related: Ensembl:ENSMUSG00000025825 AllianceGenome:MGI:1913633
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nifu; ISCU2; Nifun; 2310020H20Rik
Summary: Predicted to enable 2 iron, 2 sulfur cluster binding activity; ferrous iron binding activity; and molecular adaptor activity. Predicted to be involved in several processes, including negative regulation of iron ion import across plasma membrane; positive regulation of aconitate hydratase activity; and positive regulation of mitochondrial electron transport, NADH to ubiquinone. Located in mitochondrion. Orthologous to human ISCU (iron-sulfur cluster assembly enzyme). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in adrenal adult (RPKM 128.4), kidney adult (RPKM 126.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 55.55 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (113910479..113916349)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (113772418..113778288)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ISCU expression was decreased in the majority of human liver cancer tissues, and its reduced expression was significantly associated with p53 mutation.
Title: Regulation of iron homeostasis by the p53-ISCU pathway.
IscU is a marginally stable protein at low ionic strength to the point that undergoes cold denaturation at around -8 degrees C with a corresponding dramatic decrease of enthalpy, which is consistent with the fluxional nature of the protein.
Title: The role of zinc in the stability of the marginally stable IscU scaffold protein.
mTORC1 associates with ISCU and phosphorylates ISCU at serine 14. This phosphorylation stabilized ISCU protein.
Title: Mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation stabilizes ISCU protein: implications for iron metabolism.
Data show that complete loss of ISCU results in early embryonic death and confirm a fundamental role for ISCU in mammals.
Title: Tissue-specific splicing of ISCU results in a skeletal muscle phenotype in myopathy with lactic acidosis, while complete loss of ISCU results in early embryonic death in mice.
Results identify the iron-sulfur cluster assembly proteins (ISCU1/2) as direct targets for repression by the hypoxia-induced microRNA-210.
Title: MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2.
While IFN-gamma alone induced Nfs1 protein instability, LPS triggered a delayed decline of Nfs1, rather involving transcriptional events or mRNA instability.
Title: Regulation of the cysteine desulfurase Nfs1 and the scaffold protein IscU in macrophages stimulated with interferon-gamma and lipopolysaccharide.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

RH124652 (e-PCR)
- UniSTS:161474

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables ferrous iron binding	IBA Inferred from Biological aspect of Ancestor more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in [2Fe-2S] cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in [4Fe-4S] cluster assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of [4Fe-4S] cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in intracellular iron ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular iron ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in iron-sulfur cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in iron-sulfur cluster assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of iron ion import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of aconitate hydratase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial electron transport, NADH to ubiquinone	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
part_of iron-sulfur cluster assembly complex	NAS Non-traceable Author Statement more info	PubMed
part_of mitochondrial iron-sulfur cluster assembly complex	IDA Inferred from Direct Assay more info	PubMed
part_of mitochondrial iron-sulfur cluster assembly complex	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: iron-sulfur cluster assembly enzyme ISCU

Names: IscU iron-sulfur cluster scaffold homolog; iron-sulfur cluster assembly enzyme ISCU, mitochondrial; nifU-like N-terminal domain-containing protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001363317.1 → NP_001350246.1 iron-sulfur cluster assembly enzyme ISCU isoform 2

Status: VALIDATED

Source sequence(s)

AC132939, AI851358, AK083247

Conserved Domains (1) summary

PRK11325
Location:50 → 170

PRK11325; scaffold protein; Provisional
NM_025526.5 → NP_079802.1 iron-sulfur cluster assembly enzyme ISCU isoform 1 precursor

See identical proteins and their annotated locations for NP_079802.1

Status: VALIDATED

Source sequence(s)

AC132939

Consensus CDS

CCDS19553.1

UniProtKB/Swiss-Prot

Q4VBC7, Q8K344, Q9D7P6

Related

ENSMUSP00000026937.6, ENSMUST00000026937.12

Conserved Domains (1) summary

PRK11325
Location:36 → 159

PRK11325; scaffold protein; Provisional

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

113910479..113916349

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030254754.1 → XP_030110614.1 iron-sulfur cluster assembly enzyme ISCU isoform X1

Conserved Domains (1) summary

PRK11325
Location:117 → 221

PRK11325; scaffold protein; Provisional
XM_030254755.1 → XP_030110615.1 iron-sulfur cluster assembly enzyme ISCU isoform X2

Conserved Domains (1) summary

cl00528
Location:50 → 151

IscU_like; Iron-sulfur cluster scaffold-like proteins