Maea macrophage erythroblast attacher [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Maeaprovided by MGI
Official Full Name: macrophage erythroblast attacherprovided by MGI
Primary source: MGI:MGI:1891748
See related: Ensembl:ENSMUSG00000079562 AllianceGenome:MGI:1891748
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EMP; Gid9; 1110030D19Rik
Summary: Enables actin binding activity. Acts upstream of or within cytoskeleton organization and enucleate erythrocyte development. Located in actin cytoskeleton. Orthologous to human MAEA (macrophage erythroblast attacher, E3 ubiquitin ligase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 65.7), placenta adult (RPKM 57.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 17.55 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (33492916..33530638)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (33335572..33373294)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells.
Title: MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells.
The data showed that erythroblast macrophage protein (EMP) is ubiquitously expressed in macrophages at all of the tissues of the lupus and control mice. However, the expression of EMP in bone marrow and liver tissues of lupus mice was higher than in the control mice, which indicates that EMP may be important in the development of systemic lupus erythematosus.
Title: Differential tissue expression of erythroblast macrophage protein in a MRL/lpr mouse model of lupus.
results implicate Emp in abnormal cell motility in macrophage cells
Title: Novel interactions between erythroblast macrophage protein and cell migration.
Emp plays a cell intrinsic role in the erythroid lineage
Title: Requirement for erythroblast-macrophage protein (Emp) in definitive erythropoiesis.
Temporal and spatial expression of Emp correlates with its role in erythroblastic island formation, suggesting that Emp may be involved in multiple cellular functions.
Title: Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation.
Emp null model provides an experimental system to study enucleation process and to evaluate the function of macrophages in definitive erythropoiesis.
Title: Absence of erythroblast macrophage protein (Emp) leads to failure of erythroblast nuclear extrusion.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (4) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5Jcs35 (e-PCR), detects polymorphism
- UniSTS:478871

UniSTS:235163 (e-PCR)
- UniSTS:235163

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
contributes_to ubiquitin-protein transferase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell adhesion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within enucleate erythrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within erythrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within erythrocyte maturation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of myeloid cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of GID complex	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in actomyosin contractile ring	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in spindle	ISO Inferred from Sequence Orthology more info
part_of ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein transferase MAEA

Names: erythroblast macrophage protein

NP_067475.2

EC 2.3.2.27

XP_006504104.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001429041.1 → NP_001415970.1 E3 ubiquitin-protein transferase MAEA isoform 2

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429042.1 → NP_001415971.1 E3 ubiquitin-protein transferase MAEA isoform 3

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429043.1 → NP_001415972.1 E3 ubiquitin-protein transferase MAEA isoform 4

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429044.1 → NP_001415973.1 E3 ubiquitin-protein transferase MAEA isoform 5

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429045.1 → NP_001415974.1 E3 ubiquitin-protein transferase MAEA isoform 6

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429046.1 → NP_001415975.1 E3 ubiquitin-protein transferase MAEA isoform 7

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_001429047.1 → NP_001415976.1 E3 ubiquitin-protein transferase MAEA isoform 8

Status: VALIDATED

Source sequence(s)

AC145072, AC158923
NM_021500.3 → NP_067475.2 E3 ubiquitin-protein transferase MAEA isoform 1

See identical proteins and their annotated locations for NP_067475.2

Status: VALIDATED

Source sequence(s)

AC145072, AC158923

Consensus CDS

CCDS39063.1

UniProtKB/Swiss-Prot

Q3TFH0, Q3U7T6, Q4VC33, Q5XKE6, Q8BPI3, Q8BSA1, Q9JK49

Related

ENSMUSP00000110093.4, ENSMUST00000114449.7

Conserved Domains (3) summary

smart00667
Location:120 → 153

LisH; Lissencephaly type-1-like homology motif

cd16659
Location:336 → 383

RING-Ubox_Emp; U-box domain, a modified RING finger, found in erythroblast macrophage protein (Emp) and similar proteins

pfam10607
Location:160 → 301

CLTH; CTLH/CRA C-terminal to LisH motif domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

33492916..33530638

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006504041.2 → XP_006504104.1 E3 ubiquitin-protein transferase MAEA isoform X1

Conserved Domains (3) summary

smart00667
Location:72 → 105

LisH; Lissencephaly type-1-like homology motif

cd16659
Location:288 → 335

RING-Ubox_Emp; U-box domain, a modified RING finger, found in erythroblast macrophage protein (Emp) and similar proteins

pfam10607
Location:112 → 253

CLTH; CTLH/CRA C-terminal to LisH motif domain