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Rad18 RAD18 E3 ubiquitin protein ligase [ Mus musculus (house mouse) ]

Gene ID: 58186, updated on 11-Apr-2024

Summary

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Official Symbol
Rad18provided by MGI
Official Full Name
RAD18 E3 ubiquitin protein ligaseprovided by MGI
Primary source
MGI:MGI:1890476
See related
Ensembl:ENSMUSG00000030254 AllianceGenome:MGI:1890476
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Rad18sc; 2810024C04Rik
Summary
Predicted to enable several functions, including Y-form DNA binding activity; identical protein binding activity; and ubiquitin protein ligase binding activity. Acts upstream of or within several processes, including negative regulation of DNA recombination; response to UV; and spermatogenesis. Located in XY body; chromatin; and nucleus. Is expressed in adrenal medulla; dorsal root ganglion; testis; trunk mesenchyme derived from neural crest; and urinary system. Orthologous to human RAD18 (RAD18 E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in testis adult (RPKM 6.5), liver E14 (RPKM 4.9) and 19 other tissues See more
Orthologs
human all
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Genomic context

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See Rad18 in Genome Data Viewer
Location:
6 E3; 6 52.42 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (112596812..112673650, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (112619851..112696691, complement)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 23002 Neighboring gene BRO1 domain and CAAX motif containing pseudogene Neighboring gene STARR-seq mESC enhancer starr_17018 Neighboring gene STARR-seq mESC enhancer starr_17019 Neighboring gene STARR-seq mESC enhancer starr_17020 Neighboring gene STARR-positive B cell enhancer ABC_E8831 Neighboring gene STARR-positive B cell enhancer ABC_E11330 Neighboring gene predicted gene, 35824 Neighboring gene predicted gene, 26799

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice. Shimizu T, et al. DNA Repair (Amst), 2017 Feb. PMID 28082021
  2. Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo. Yang Y, et al. Nucleic Acids Res, 2016 May 19. PMID 26883629, Free PMC Article
  3. Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure. Tanoue Y, et al. J Invest Dermatol, 2018 Dec. PMID 29859927
  4. RAD18 activates the G2/M checkpoint through DNA damage signaling to maintain genome integrity after ionizing radiation exposure. Sasatani M, et al. PLoS One, 2015. PMID 25675240, Free PMC Article
  5. Rad18 is required for long-term maintenance of spermatogenesis in mouse testes. Sun J, et al. Mech Dev, 2009 Mar-Apr. PMID 19068231

See all (43) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
    Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
  2. these results demonstrate that the tumor-suppressive role of DNA polymerase Eta in UV-treated skin is Rad18 independent
    Title: Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure.
  3. Somatic hypermutation of immunoglobulin genes (SHM) in Rad18 KO mice was similar to wild-type mice, suggesting that Rad18 is dispensable for SHM.
    Title: Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice.
  4. Rad18 confers DNA damage tolerance and tumor-suppression in a physiological setting.
    Title: Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo.
  5. REV1 promote PCNA monoubiquitylation after UV radiation through interacting with ubiquitylated RAD18.
    Title: REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18.
  6. RAD18 can function as a mediator for DNA damage response signals to activate the G2/M checkpoint in order to maintain genome integrity and cell survival after IR exposure.
    Title: RAD18 activates the G2/M checkpoint through DNA damage signaling to maintain genome integrity after ionizing radiation exposure.
  7. repairs meiotic double-strand DNA breaks and and maintains meiotic sex chromosome inactivation
    Title: Meiotic functions of RAD18.
  8. RAD18 is intrinsically required for the long-term maintenance of spermatogenesis.
    Title: Rad18 is required for long-term maintenance of spermatogenesis in mouse testes.
  9. Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA.
    Title: Effect of DNA repair protein Rad18 on viral infection.
  10. RAD18 contributes to maintenance of genomic stability through postreplication repair
    Title: Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables Y-form DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables polyubiquitin modification-dependent protein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-containing complex binding ISO
Inferred from Sequence Orthology
more info
  
enables single-stranded DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA damage response ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in DNA damage response ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of chromosome segregation ISO
Inferred from Sequence Orthology
more info
  
involved_in postreplication repair IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein autoubiquitination ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in protein monoubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within response to UV IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within spermatogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Rad6-Rad18 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in XY body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in centrosome ISO
Inferred from Sequence Orthology
more info
  
part_of chromatin IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in male germ cell nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear body ISO
Inferred from Sequence Orthology
more info
  
located_in nuclear inclusion body ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in replication fork ISO
Inferred from Sequence Orthology
more info
  
located_in site of double-strand break ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase RAD18
Names
RAD18 homolog
RING-type E3 ubiquitin transferase RAD18
mRAD18Sc
post-replication repair protein RAD18SC
postreplication repair protein RAD18
NP_001161202.1
NP_001368860.1
NP_001368861.1
NP_001368862.1
NP_067360.2
XP_006506497.1
XP_006506500.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001167730.1 → NP_001161202.1  E3 ubiquitin-protein ligase RAD18 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC157095, AK028505, AK030043
    Consensus CDS
    CCDS51870.1
    UniProtKB/TrEMBL
    E9Q392, Q8CDH2
    Related
    ENSMUSP00000070619.6, ENSMUST00000068487.12
    Conserved Domains (4) summary
    smart00734
    Location:201 → 224
    ZnF_Rad18; Rad18-like CCHC zinc finger
    cd00162
    Location:25 → 63
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    TIGR00599
    Location:13 → 310
    rad18; DNA repair protein rad18
    pfam02037
    Location:248 → 282
    SAP; SAP domain

  2. NM_001381931.1 → NP_001368860.1  E3 ubiquitin-protein ligase RAD18 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC153591, AC157095
    UniProtKB/TrEMBL
    Q8CDH2
    Related
    ENSMUSP00000108807.2, ENSMUST00000113182.8
    Conserved Domains (1) summary
    TIGR00599
    Location:13 → 363
    rad18; DNA repair protein rad18

  3. NM_001381932.1 → NP_001368861.1  E3 ubiquitin-protein ligase RAD18 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC153591, AC157095
    UniProtKB/TrEMBL
    Q8CDH2
    Conserved Domains (1) summary
    TIGR00599
    Location:13 → 310
    rad18; DNA repair protein rad18

  4. NM_001381933.1 → NP_001368862.1  E3 ubiquitin-protein ligase RAD18 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC153591, AC157095
    Conserved Domains (1) summary
    TIGR00599
    Location:58 → 231
    rad18; DNA repair protein rad18

  5. NM_021385.2 → NP_067360.2  E3 ubiquitin-protein ligase RAD18 isoform 2

    See identical proteins and their annotated locations for NP_067360.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AC157095, AK012795
    Consensus CDS
    CCDS39589.1
    UniProtKB/Swiss-Prot
    Q9CZB8, Q9QXK2
    UniProtKB/TrEMBL
    Q8CDH2
    Related
    ENSMUSP00000076341.5, ENSMUST00000077088.11
    Conserved Domains (4) summary
    smart00734
    Location:201 → 224
    ZnF_Rad18; Rad18-like CCHC zinc finger
    cd00162
    Location:25 → 63
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    TIGR00599
    Location:13 → 363
    rad18; DNA repair protein rad18
    pfam02037
    Location:248 → 282
    SAP; SAP domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    112596812..112673650 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006506437.5 → XP_006506500.1  E3 ubiquitin-protein ligase RAD18 isoform X2

    Related
    ENSMUSP00000108805.2, ENSMUST00000113180.8
    Conserved Domains (1) summary
    TIGR00599
    Location:13 → 310
    rad18; DNA repair protein rad18

  2. XM_006506434.5 → XP_006506497.1  E3 ubiquitin-protein ligase RAD18 isoform X1

    UniProtKB/TrEMBL
    Q8CDH2
    Conserved Domains (1) summary
    TIGR00599
    Location:13 → 310
    rad18; DNA repair protein rad18
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9QXK2.2 GenPept UniProtKB/Swiss-Prot:Q9QXK2

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