Dhodh dihydroorotate dehydrogenase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dhodhprovided by MGI
Official Full Name: dihydroorotate dehydrogenaseprovided by MGI
Primary source: MGI:MGI:1928378
See related: Ensembl:ENSMUSG00000031730 AllianceGenome:MGI:1928378
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2810417D19Rik
Summary: Enables dihydroorotase activity and dihydroorotate dehydrogenase activity. Involved in 'de novo' UMP biosynthetic process and UDP biosynthetic process. Located in mitochondrion. Is expressed in several structures, including alimentary system; branchial arch; limb bud; respiratory system; and urinary system. Human ortholog(s) of this gene implicated in postaxial acrofacial dysostosis. Orthologous to human DHODH (dihydroorotate dehydrogenase (quinone)). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 7.7), CNS E11.5 (RPKM 7.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 D3; 8 57.12 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (110319876..110335330, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (109593244..109608699, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Targeting DHODH reveals therapeutic opportunities in ATRA-resistant acute promyelocytic leukemia.
Title: Targeting DHODH reveals therapeutic opportunities in ATRA-resistant acute promyelocytic leukemia.
Knockdown of Dhodh reduced hippocampus mean firing rate.
Title: Mitochondrial Regulation of the Hippocampal Firing Rate Set Point and Seizure Susceptibility.
DHODH inhibition led to a marked decrease in melanoma growth both in vitro and in mouse xenograft studies
Title: DHODH modulates transcriptional elongation in the neural crest and melanoma.
Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells of different species
Title: Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells.
immunosuppressive agent HR325 (an inhibitor of dihydroorotate dehydrogenase, DHODH) inhibits immunoglobulin (Ig) secretion both in vitro and in vivo and that this effect can be reverse with exogenous uridine
Title: In vitro and in Vivo inhibition of immunoglobulin secretion by the immunosuppressive compound HR325 is reversed by exogenous uridine.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2)
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AF029667 (e-PCR)
- UniSTS:159813

Dhodh (e-PCR), detects polymorphism
- UniSTS:547740

Dhodh (e-PCR), detects polymorphism
- UniSTS:546738

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables FMN binding	ISO Inferred from Sequence Orthology more info
enables dihydroorotase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables dihydroorotate dehydrogenase (quinone) activity	IEA Inferred from Electronic Annotation more info
enables dihydroorotate dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydroorotate dehydrogenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables dihydroorotate dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-CH group of donors	IEA Inferred from Electronic Annotation more info
enables quinone binding	ISO Inferred from Sequence Orthology more info
enables ubiquinone binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in UDP biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pyrimidine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in pyrimidine ribonucleotide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mitochondrial fission	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dihydroorotate dehydrogenase (quinone), mitochondrial

Names: DHOdehase; dihydroorotate dehydrogenase, mitochondrial; dihydroorotate oxidase

NP_064430.1

EC 1.3.5.2

XP_036010093.1

EC 1.3.5.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_020046.3 → NP_064430.1 dihydroorotate dehydrogenase (quinone), mitochondrial precursor

See identical proteins and their annotated locations for NP_064430.1

Status: VALIDATED

Source sequence(s)

AC125162, BC045206

Consensus CDS

CCDS40471.1

UniProtKB/Swiss-Prot

O35435

Related

ENSMUSP00000115934.2, ENSMUST00000123605.9

Conserved Domains (2) summary

PLN02826
Location:4 → 394

PLN02826; dihydroorotate dehydrogenase

cd04738
Location:43 → 373

DHOD_2_like; Dihydroorotate dehydrogenase (DHOD) class 2. DHOD catalyzes the oxidation of (S)-dihydroorotate to orotate. This is the fourth step and the only redox reaction in the de novo biosynthesis of UMP, the precursor of all pyrimidine nucleotides. DHOD requires ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

110319876..110335330 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154200.1 → XP_036010093.1 dihydroorotate dehydrogenase (quinone), mitochondrial isoform X1

Conserved Domains (1) summary

cd04738
Location:2 → 332

DHOD_2_like; Dihydroorotate dehydrogenase (DHOD) class 2. DHOD catalyzes the oxidation of (S)-dihydroorotate to orotate. This is the fourth step and the only redox reaction in the de novo biosynthesis of UMP, the precursor of all pyrimidine nucleotides. DHOD requires ...