Akap8 A kinase anchor protein 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Akap8provided by MGI
Official Full Name: A kinase anchor protein 8provided by MGI
Primary source: MGI:MGI:1928488
See related: Ensembl:ENSMUSG00000024045 AllianceGenome:MGI:1928488
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: AKAP-8; AKAP95; 1200016A02Rik
Summary: This gene encodes a member of the A-kinase anchoring protein (AKAP) family. These proteins are characterized by their ability to bind to the R subunit of protein kinase A (PKA) and anchor the protein at different subcellular locations. This protein has been shown to regulate apoptosis and to be involved in palatogenesis. Knockdown of this gene has been associated with altered histone modifications and reduced expression of developmental genes in mouse embryonic stem cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 24.6), CNS E14 (RPKM 20.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 B1; 17 17.48 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (32522646..32540212, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (32303672..32321238, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
Title: Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
This study showed an increase in the level of global DNA methylation starting in oocytes with diameters around 60 microm, increases in histone h4, and decreases in AKAP95.
Title: DNA methylation, histone modifications and behaviour of AKAP95 during mouse oocyte growth and upon nuclear transfer of foreign chromatin into fully grown prophase oocytes.
a specific role for A kinase-anchoring protein 95 in suppressing the expression of the gene encoding tumor necrosis factor-alpha, which involved phosphorylation of p105 (also known as Nfkb1) by PKA
Title: Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (1)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH124529 (e-PCR)
- UniSTS:161351

Akap8 (e-PCR), detects polymorphism
- UniSTS:506721

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

AU015639 (e-PCR)
- UniSTS:209291

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables NF-kappaB binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase A regulatory subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A regulatory subunit binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell cycle G2/M phase transition	ISO Inferred from Sequence Orthology more info
involved_in cellular response to lipopolysaccharide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to prostaglandin E stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mitotic chromosome condensation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within mitotic chromosome condensation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of tumor necrosis factor production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of chromatin	ISO Inferred from Sequence Orthology more info
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in female pronucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear matrix	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: A-kinase anchor protein 8

Names: A kinase (PRKA) anchor protein 8; A-kinase anchor protein 95 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357760.1 → NP_001344689.1 A-kinase anchor protein 8 isoform 2

Status: REVIEWED

Source sequence(s)

BC060170, CT033751

Conserved Domains (1) summary

pfam04988
Location:273 → 433

AKAP95; A-kinase anchoring protein 95 (AKAP95)
NM_019774.5 → NP_062748.2 A-kinase anchor protein 8 isoform 1

See identical proteins and their annotated locations for NP_062748.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the shortest transcript and encodes the protein.

Source sequence(s)

AK004801, BC060170, BC060209

Consensus CDS

CCDS37555.1

UniProtKB/Swiss-Prot

Q9DBR0, Q9R0L8

UniProtKB/TrEMBL

Q059U9

Related

ENSMUSP00000002699.6, ENSMUST00000002699.7

Conserved Domains (1) summary

pfam04988
Location:387 → 547

AKAP95; A-kinase anchoring protein 95 (AKAP95)

RNA

NR_131218.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

BC060170, BC060209

Related

ENSMUST00000237381.2
NR_131219.1 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks two alternate internal exons and uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

BC054433, BC060170, BC060209

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

32522646..32540212 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006524665.5 → XP_006524728.1 A-kinase anchor protein 8 isoform X2

See identical proteins and their annotated locations for XP_006524728.1

Conserved Domains (1) summary

pfam04988
Location:273 → 433

AKAP95; A-kinase anchoring protein 95 (AKAP95)
XM_006524666.5 → XP_006524729.1 A-kinase anchor protein 8 isoform X2

See identical proteins and their annotated locations for XP_006524729.1

Conserved Domains (1) summary

pfam04988
Location:273 → 433

AKAP95; A-kinase anchoring protein 95 (AKAP95)
XM_006524663.5 → XP_006524726.1 A-kinase anchor protein 8 isoform X1

Conserved Domains (1) summary

pfam04988
Location:362 → 522

AKAP95; A-kinase anchoring protein 95 (AKAP95)