Skap2 src family associated phosphoprotein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Skap2provided by MGI
Official Full Name: src family associated phosphoprotein 2provided by MGI
Primary source: MGI:MGI:1889206
See related: Ensembl:ENSMUSG00000059182 AllianceGenome:MGI:1889206
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RA70; Saps; Scap2; SKAP-HOM; mSKAP55R; 2610021A10Rik
Summary: Acts upstream of or within negative regulation of cell population proliferation. Located in cytoplasm. Orthologous to human SKAP2 (src kinase associated phosphoprotein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in placenta adult (RPKM 10.2), liver E18 (RPKM 9.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 B3; 6 25.35 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (51836145..51989529, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (51859165..52012549, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.
Title: SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.
These findings reveal that YopH prevents activation of both SKAP2-dependent and -independent neutrophilic defenses, uncouple integrin- and GPCR-dependent ROS production from FcgammaR responses based on their SKAP2 dependency, and show that SKAP2 is not required for degranulation.
Title: Yersinia pseudotuberculosis YopH targets SKAP2-dependent and independent signaling pathways to block neutrophil antimicrobial mechanisms during infection.
SKAP2 depletion also disrupted actin cap and cortical granule-free domain formation, and arrested a large proportion of oocytes at the telophase stage. Moreover, Arp2/3 complex and WAVE2 expression was decreased after the depletion of SKAP2 activity.
Title: SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.
Skap2 is essential to activate the beta2 integrins, and loss of Skap2 function is sufficient to cause a leukocyte adhesion deficiency (LAD)-like phenotype in mice.
Title: Skap2 is required for β(2) integrin-mediated neutrophil recruitment and functions.
Macrophages assemble the appropriate motile machinery to infiltrate tumors and promote disease progression, and implicate SKAP2 as an attractive candidate for therapeutically targeting TAMs.
Title: SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.
RA70/Scap2 may be necessary for RA-induced neuronal differentiation from the posterior neuroectoderm.
Title: Spatial and temporal expression of RA70/Scap2 in the developing neural tube.
a novel role of SKAP-Hom and its phosphorylation in the regulation of cellular motility.
Title: Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.
integrin activation by the ADAP-SKAP55-signaling module controls the stability and duration of T cell-dendritic cell contacts during the progressive phases necessary for optimal T cell activation.
Title: Multistage T cell-dendritic cell interactions control optimal CD4 T cell activation through the ADAP-SKAP55-signaling module.
Sirpalpha is not exclusively immunoinhibitory, and illuminate previously unexplained observations implicating Skap2 and Sirpalpha in mouse models of inflammatory disease.
Title: Macrophages require Skap2 and Sirpα for integrin-stimulated cytoskeletal rearrangement.
These results revealed an essential role for HSF4-mediated SKAP2 expression in the regulation of actin reorganization during lens differentiation.
Title: SKAP2, a novel target of HSF4b, associates with NCK2/F-actin at membrane ruffles and regulates actin reorganization in lens cell.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (3) 1 citation
Targeted (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AA960083 (e-PCR)
- UniSTS:159506

RH118301 (e-PCR)
- UniSTS:160949

24.MMHAP36FLH3.seq (e-PCR)
- UniSTS:123642

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell activation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: src kinase-associated phosphoprotein 2

Names: SKAP55 homolog; SKAP55 homologue; Src-associated adaptor protein; pyk2/RAFTK-associated protein; src kinase-associated phosphoprotein 55-related protein; src-associated adapter protein with PH and SH3 domains

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_018773.2 → NP_061243.1 src kinase-associated phosphoprotein 2

See identical proteins and their annotated locations for NP_061243.1

Status: PROVISIONAL

Source sequence(s)

BC003711

Consensus CDS

CCDS20137.1

UniProtKB/Swiss-Prot

Q3UND0, Q8BK74, Q9Z2K4

Related

ENSMUSP00000145462.2, ENSMUST00000204778.3

Conserved Domains (3) summary

cd13381
Location:117 → 222

PH_Skap-hom_Skap2; Src kinase-associated phosphoprotein homolog and Skap 2 Pleckstrin homology (PH) domain

pfam00169
Location:117 → 215

PH; PH domain

cl17036
Location:300 → 352

SH3; Src Homology 3 domain superfamily