E2f6 E2F transcription factor 6 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: E2f6provided by MGI
Official Full Name: E2F transcription factor 6provided by MGI
Primary source: MGI:MGI:1354159
See related: Ensembl:ENSMUSG00000057469 AllianceGenome:MGI:1354159
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EMA; E2F6a; E2F6b
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Acts upstream of or within regulation of transcription involved in G1/S transition of mitotic cell cycle. Predicted to be located in nucleoplasm. Predicted to be part of MLL1 complex and RNA polymerase II transcription regulator complex. Is expressed in several structures, including alimentary system; genitourinary system; limb; nervous system; and sensory organ. Orthologous to human E2F6 (E2F transcription factor 6). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 10.4), heart adult (RPKM 9.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 A1.1; 12 8.04 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (16860932..16876753)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (16810909..16826752)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MicroRNA-31 regulates TNF-alpha and IL-17A co-induced-endothelial cell apoptosis by repressing E2F6.
Title: MicroRNA-31 regulates TNF-α and IL-17A co-induced-endothelial cell apoptosis by repressing E2F6.
Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
Title: Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.
E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.
Title: E2F6 initiates stable epigenetic silencing of germline genes during embryonic development.
The results strongly suggest that TLX acts through E2F6 to regulate islet beta cell proliferation.
Title: Nuclear receptor TLX regulates islet beta cell proliferation via E2F6.
E2F6 suppresses Mdm2 expression in cells harboring the SNP309G allele but not the SNP309T allele.
Title: Contrasting effects of an Mdm2 functional polymorphism on tumor phenotypes.
By comparison in HeLa, Dox induced apoptosis through upregulation of endogenous E2F1 involving post-transcriptional mechanisms, while E2F6 was down regulated with induction of the Checkpoint kinase-1 and proteasome degradation. These data imply that E2F6 serves to modulate E2F activity and protect cells including cardiomyocytes from apoptosis and improve survival.
Title: E2F6 protein levels modulate drug induced apoptosis in cardiomyocytes.
These data reveal a novel interplay between the E2F pathway, beta2-adrenergic/PKA/PDE4D, and ERK/c-Src axis in fine tuning the pathological hypertrophic growth response.
Title: Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium.
E2f6 was similarly bound to the proximal promoter regions both in embryonic stem cells (ESCs) and epiblast stem cells (EpiSCs)
Title: E2f6-mediated repression of the meiotic Stag3 and Smc1β genes during early embryonic development requires Ezh2 and not the de novo methyltransferase Dnmt3b.
possesses a broad ability to bind to and regulate the meiosis-specific gene population
Title: A conserved E2F6-binding element in murine meiosis-specific gene promoters.
This analysis shows that E2f6 and Bmi1 cooperate in the regulation of Hox genes, and consequently axial skeleton development, but not in the repression of the Ink4a-Arf locus.
Title: E2f6 and Bmi1 cooperate in axial skeletal development.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI462434 (e-PCR)
- UniSTS:160185

E2f6 (e-PCR), detects polymorphism
- UniSTS:265705

E2f6 (e-PCR), detects polymorphism
- UniSTS:506871

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication factor A complex	IC Inferred by Curator more info	PubMed
part_of MLL1 complex	ISO Inferred from Sequence Orthology more info
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II transcription regulator complex	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcription factor E2F6

Names: E2F-6; E2F-binding site-modulating activity protein; transcriptional repressor E2F6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_033270.2 → NP_150373.2 transcription factor E2F6

See identical proteins and their annotated locations for NP_150373.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the protein-coding transcript.

Source sequence(s)

AI851823, BC037656, BY739130

Consensus CDS

CCDS36409.1

UniProtKB/Swiss-Prot

O54917, Q8K456

UniProtKB/TrEMBL

Q8VHT5

Related

ENSMUSP00000020908.8, ENSMUST00000020908.9

Conserved Domains (2) summary

cd14660
Location:139 → 239

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:65 → 128

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

RNA

NR_027677.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains an additional, out-of-frame, exon at the 5' end compared to variant 1. It is represented as non-coding because the use of the 5'-most translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AI851823, AK146792, CJ075693

Related

ENSMUST00000221541.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

16860932..16876753

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006515147.2 → XP_006515210.1 transcription factor E2F6 isoform X1

See identical proteins and their annotated locations for XP_006515210.1

UniProtKB/TrEMBL

Q8VHT4, Q8VHT5

Conserved Domains (2) summary

cd14660
Location:104 → 204

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:29 → 93

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_036157521.1 → XP_036013414.1 transcription factor E2F6 isoform X2

UniProtKB/TrEMBL

Q8VHT5

Conserved Domains (2) summary

cd14660
Location:87 → 187

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:13 → 76

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_006515148.3 → XP_006515211.1 transcription factor E2F6 isoform X1

See identical proteins and their annotated locations for XP_006515211.1

UniProtKB/TrEMBL

Q8VHT4, Q8VHT5

Conserved Domains (2) summary

cd14660
Location:104 → 204

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:29 → 93

E2F_TDP; E2F/DP family winged-helix DNA-binding domain
XM_006515149.5 → XP_006515212.1 transcription factor E2F6 isoform X2

UniProtKB/TrEMBL

Q8VHT5

Conserved Domains (2) summary

cd14660
Location:87 → 187

E2F_DD; Dimerization domain of E2F transcription factors

pfam02319
Location:13 → 76

E2F_TDP; E2F/DP family winged-helix DNA-binding domain